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Comparison, identification and characterization of endophytic bacteria from Chinese flowering cabbage (Brassica parachinensis) by soil and soilless culture*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 06
Page:
1-12
Research Field:
Articles
Publishing date:

Info

Title:
Comparison, identification and characterization of endophytic bacteria from Chinese flowering cabbage (Brassica parachinensis) by soil and soilless culture*
Author(s):
GU Licheng1 HUANG Caiyi1 HUANG Shufen1 JIANG Baoru1 TAN Shuya1 CHEN Junyi1 PENG Guixiang2 & TAN Zhiyuan1
1 College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
Keywords:
soilless culture soil culture Brassica parachinensis endophytic bacteria relative chlorophyll content (SPAD) soluble sugar content
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.03050
DocumentCode:

Abstract:
Chinese flowering cabbage ( Brassica parachinensis) is a kind of special vegetable in Southern China. Several endophytic bacteria strains were isolated from soil and soilless cultivated Chinese flowering cabbage respectively to identify the difference of between those two materials . The isolated strains were divided to several groups according to IS-PCR DNA fingerprinting patterns. Then 16S rRNA gene sequences of representative strains were aligned for phylogeny analysis. The bacterial fluid of representative strains was added into the soilless culture nutrient solution. After that, the relative chlorophyll content (SPAD) and soluble sugar content of each group was measured. As a result, 31 isolates were acquired respectively from those two types of materials in total. They were divided into 9 groups with IS-PCR fingerprinting method. 16S rRNA gene sequences of representative strains were analyzed, which implied they belonged to Bacillus sp., Rhizobium sp., Microbacterium sp., Leifsonia sp., Sphingomonas sp., Alcaligenes sp. and Agrobacterium sp. Through the plate counting method, results proved that there are more culturable endophytic bacteria inside the soil cultured Chinese flowering cabbage than the soilless cultured Chinese flowering cabbage. What’s more, the Bio-BIQA board test result indicated that the carbon metabolism of microbes from the soil cultured Chinese flowering cabbage had more diversity than those from those soilless cultured ones. The following physiological and biochemical tests revealed that some strains had the ability of secreting indole acetic acid and producing siderophore. After adding the bacterial fluid of strain SCG14 and SCG0802 into the soilless culture nutrient solution, the relative chlorophyll content and soluble sugar content of Chinese flowering cabbage performed a significant promotion than the control group. This experiment showed that the endophytic bacteria in soilless cultured Chinese flowering cabbage and those in soil cultured Chinese flowering cabbage are different in types, numbers, distribution and carbon metabolism. Besides, the strains isolated could enhance the relative chlorophyll content (SPAD) and soluble sugar content of soilless cultured Chinese flowering cabbage, which have a prospect of developing new microbial preparation.

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Last Update: 2019-05-14