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Effects of exogenous NO on seed germination and ASA-GSH in leaves of Zizyphus jujuba seedlings under cold stress*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 06
Page:
1-16
Research Field:
Articles
Publishing date:

Info

Title:
Effects of exogenous NO on seed germination and ASA-GSH in leaves of Zizyphus jujuba seedlings under cold stress*
Author(s):
MA Yunxia1 WANG Yuelin2 LI Gangtie1** LIANG Tianyu 1 ZOU Miao 1 YAN Jinqiuzi1 & MA Hui1
1 College of Desert Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
2 Grassland Workstation of Inner Mongolia Autonomous Region, Hohhot 010017, China
Keywords:
NO Cold stress Zizyphus jujuba Seed AsA-GSH circulatory system
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.03039
DocumentCode:

Abstract:
Through artificial indoor simulation of cold stress, 0.1?0.2?0.5 mmol/L Sodium nitroferricyanide(III) dehydrate(Sodium nitroprusside, SNP) as exogenous No donor and nitric oxide synthase inhibitor (L-NAME), nitrate reductase non-specific inhibitor (NaN3) and nitric oxide scavenger (cPTIO) were used to treat seedlings and leaves of Zizyphus jujuba, in order to study its effects on germination characteristics of Zizyphus jujuba seeds and ascorbate glutathione (ASA-GSH) circulation system of Zizyphus jujuba seedlings under cold stress and provide theoretical basis for the extensive application of exogenous nitric oxide (NO) in the future stress-resistant production of Zizyphus jujuba .The results showed that under 4 ℃ cold stress, seed germination and seedling growth of Zizyphus jujuba were inhibited. After applying different concentrations of SNP, the germination rate, germination potential, germination index and vigor index of Zizyphus jujuba seeds were significantly increased, and decreased the contents of malondialdehyde (MDA) and hydrogen peroxide (H 2O2) in seedling leaves. At the same time, the contents of proline (Pro) was increased, ascorbic acid (AsA), glutathione (GSH) content and antioxidant enzyme activities [ascorbic peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase(MDAR)] were significantly increased. In conclusion, exogenous NO could alleviate the inhibition of cold stress on seed germination and seedling growth of Zizyphus jujuba, reduce the degree of membrane lipid peroxidation caused by cold stress, maintain the stability of AsA-GSH circulatory system and enhance cold resistance. The best mitigation effect of cold stress was 0.5 mmol/L SNP . When NO scavenger and two synthetic inhibitors were used, germination of Zizyphus jujuba seeds under cold injury stress after NO treatment could be inhibited, and the parameters of membrane lipid peroxidation of seedlings were decreased and the indexes of antioxidant enzyme and antioxidant were increased. The effect of NO on Seed Germination and seedling growth of Zizyphus jujuba under cold stress was significantly weakened, which further proved the mitigation effect of NO on Zizyphus jujuba seeds and seedlings under cold stress.

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Last Update: 2019-04-30