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Effects of phytohormones and substrate concentration on Chlorella vulgaris growth and lipid accumulation*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 06
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Effects of phytohormones and substrate concentration on Chlorella vulgaris growth and lipid accumulation*
LUO Chuan1 REN Lipin 1** ZHANG Zhi2 NIE Yudong3
1College of Environmental Science and Engineering, China West Normal University , Nanchong 637009, China
2College of Urban Construction and Environmental Engineering, Chongqing University , Chongqing 401331, China
3Engineering Research Center for Waste Oil Recovery Technology and Equipment of Education, Chongqing Technology and Business University, Chongqing 400060, China
Chlorella vulgari s Biodiesel Phytohormone Lipid yield Ecological-economic efficiency analysis

Objectives: The use of phytohormones to i ncrease the growth rate of microalgae and the rate of oil synthesis are one of the effective approach to decrease the cost of microalgae biodiesel development. Methods: Comparative study on the effects of different concentrations of natural Phytohormone Indole-3-acetic acid (IAA) and synthetic hormone 1-Naphthaleneacetic acid (NAA) as microalgae culture additives on the growth and lipid accumulation of Chlorella vulgaris. Using BG11 medium as culture substrate, were compared the effects of different substrate concentrations (10%, 25%, 50%, 75%, 100%) on the application of phytohormone were studied in the present work. Results: The results showed that the two plant hormones had significant effects on the growth and lipid synthesis of C. vulgaris (p < 0.05). The dose of 2 mg/L IAA had a better growth promoting effect than 2 mg/L NAA. After 15 d culture, the biomass obtained was (525.87 ± 5.91) mg/L and (367.72 ± 4.1) mg/L respectively, which were 2.48-fold and 1.7-fold higher than that of the control groups; The concentrations of IAA and NAA were linearly correlated with lipid accumulation. IAA showed a neutral lipid accumulation at 0.05-2 mg/L while NAA showed lipid accumulation at 1-5 mg/L; The biomass yield and oil yield of C. vulgaris were the highest after incuction by 2 mg/L IAA and 2 mg/L NAA with the figure of (35.06 ± 0.39) and (24.52 ± 0.27) mg/(L·d), (21.96 ± 0.62) and (14.38 ± 0.09) mg/(L·d) resceptively. Comparing with the control group, these figures increased by 59.68%, 41.23%, 74.99%, and 61.78%, respectively and the accumulation of C.vulgaris growth and lipid were significantly increased in different substrate concentrations, the difference was significant (p < 0.05), but the effect on the application of phytohormone was not significant. Conclusions: This study shows that phytohormones have a great influence on the microalgae culture system. The application of low concentration IAA and NAA can help to improve the efficiency of the culture system, and meet the requirements of eco-efficiency. However, from the perspective of economic efficiency, 2 mg/L NAA is more suitable as a phytohormone additive for C. vulgari culture.




Last Update: 2019-06-19