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Screening of spent mushroom substrates with high laccase activity and their decolorization of dyes *(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 06
Page:
1-12
Research Field:
Articles
Publishing date:

Info

Title:
Screening of spent mushroom substrates with high laccase activity and their decolorization of dyes *
Author(s):
LIU Zilu1 SUN Yue1 ZHANG Guoqing1** CHEN Qingjun2 XU xin1 HU Boyang1 GUAN tikun1 & LI Xiaozhen 1
1 College of Biological Science and Engineering, Beijing University of Agriculture, Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture and Rural Affairs , Beijing 102206, China
2 Plant science and technology college, Beijing University of Agriculture, Beijing 102206, China
Keywords:
spent mushroom substrate Pleurotus eryngii laccase dye decolourization
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.02031
DocumentCode:

Abstract:
Spent mushroom substrate (SMS) is the co-product referring to the cultural medi a after mushroom harvest. It is rich in nutrients such as mycelia proteins, enzymes, and polysaccharides, and used as potential bioresources in fertilizer, feed, bioremediation and other fields. China is the world’s largest producer of edible mushrooms, while a large amount of microbial resources have not been used scientifically and effectively, and cause environmental pollution. In the present study, 9 kinds of SMS were collected from factory, laboratory, and agricultural cultivation modes, such as Pleurotus eryngii, Pleurotus ostreatus, etc. Substrates with high laccase activity were screened, followed by decolorization and detoxification assays toward 5 kinds of dyes including malachite green (MG), r eactive brilliant blue (RBB), m ethyl orange (MO), r eactive red (RR), and r eactive black (RB). The results showed that SMS of P. eryngii by industrialized cultivation demonstrated the highest laccase activity of 7.55 × 10 4 IU/g. Decolorization activity using P. erngii–factory substrate (PEFS) of different height column revealed that a column of 15 cm high possessed the highest decolorization ratios towards the five dyes of 100%, 98.60%, 72.82%, 61.97%, and 43.73%, respectively. Analyzed by full-wavelength spectral scanning, the maximum light absorption of MG and RBB was significantly reduced or even disappeared. Furthermore, crude enzyme extract of PEFS manifested the decolorization ratios of 81.24%, 49.43%, 37.62%, 26.34%, and 13.54%, respectively towards the five dyes after 24 h incubation at 30 °C. Toxicity tests of the enzymatic degradation products of dyes were performed using Escherichia coli and Staphylococcus aureus as targets. The results showed that the products were low-toxicity. In summary, PEFS manifests high laccase activity, and can be applied in decolorization and detoxification of dye wastewater such as MG and RBB. It possesses great potential in economic and ecological applications.

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Last Update: 2019-04-16