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Response of microbial community structure in eutrophic Taihu sediments to environmental factors*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 06
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Response of microbial community structure in eutrophic Taihu sediments to environmental factors*
WU Ling1 2**
1 Institute of Pharmaceutical and Environmental Engineering, Changzhou Vocational Institute of Engineering, Changzhou 213164, China
2 Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, School of Geography Sciences, Nanjing Normal University, Nanjing 210023, China
microbial diversity metagenomic sequencing technology eutrophic Taihu Lake sediment environmental factors

s The sediment is the important component of the aquatic ecosystem, and the study of microbial diversity in sediments is an important way to evaluate the water quality. This paper aimed to study the microbial community structure in the sediments from areas of Taihu Lake (China) with contrasting degrees of eutrophication, and the effect of main physicochemical indexes on the community structures. The surface sediments (0 ~ - 2 cm) were collected from MeiLiang Bay (ML) and Huxin (HX ) of Taihu Lake, and the total organic carbon (TOC), total nitrogen (TN) and inorganic nitrogen (NH4+-N and NO3--N), pH, redox potential (Eh) and dissolved oxygen (DO) were investigated. Moreover, Illumina Miseq metagenomic sequencing technology was applied to disclose the microbial community structure in different sediment samples, and the potential relationship between microbial community structure and physicochemical factors was also studied. Total 234 408 valid reads from 6 sediments were obtained, with Sobs index of 1811-2442, Shannon index of 6.16-6.49 , and coverage of 96.2%-97.7%. These suggested the sufficient sequence data and the high community diversity. The relative abundance of bacteria in the sediments from sampling areas of Taihu Lake contributed to more than 99% of total sequences, with a total of 48 phyla and 113 classes . Total reads in 6 sediments were mainly affiliated with proteobacteria, which were dominated by two classes of δ-proteobacteria and β-proteobacteria, and the relative proportions of proteobacteria in ML and HX were 40.5% and 35.4%, respectively . Other abundant groups include phylum Chloroflexi, Nitrospirae, Bacteroidetes and so on. Eh, DO, pH, ammonium nitrogen, and TOC values can significantly affect microbial groups on class level (OUT >1%), among which Eh is the factor affecting the largest number of microbial groups based on the Spearman correlation analysis of microbial community structures on class level and physiochemical indexes of sediments. Studies exhibited that there are abundant microbial resources in sediments of Taihu Lake , and physiochemical indexes of different eutrophic areas are the important factors for the microbial community structure.




Last Update: 2019-03-29