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Biotransformation of phytosterols to 9-hydroxyandrostenedione by Mycobacterium sp. LY-1 in oil-water two-phase system*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 05
Page:
1-8
Research Field:
Articles
Publishing date:

Info

Title:
Biotransformation of phytosterols to 9-hydroxyandrostenedione by Mycobacterium sp. LY-1 in oil-water two-phase system*
Author(s):
ZHOU Longfei1 LI Hui1** XU Yanan1 ZHANG Xiaomei1 SHI Jinsong1 & XU Zhenghong 2 3
1 School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China
2 Key Laboratory of Industrial Biotechnology, Ministry of Education, College of Bioengineering, Jiangnan University , Wuxi 214122, China
3 National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China
Keywords:
Mycobacterium sp. LY-1 phytosterol 9-Hydroxyandrost-4-ene-317-dione Tween-80 Soybean oil
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.01054
DocumentCode:

Abstract:
9α-Hydroxyandrost-4-ene-3,17-dione (9α-OH-AD), via the microbial conversion of phytosterols, is an important precursor in the manufacture of numbers of modern glucocorticoid and sex hormone drugs. The solubility of phytosterols was extremely low in water, and the bioavailability was not high, which seriously restricts the utilization efficiency of microorganisms on phytosterols. To increase the molar yield of 9α-OH-AD further, Mycobacterium sp. LY-1 was used as a starting strain by the previous mutagenesis, and the effects of the surfactants with different HLB values on the biotransformation efficacy of phytosterols to 9 α-OH-AD were investigated, the most suitable surfactant which was chosen and a highly efficient conversion system of oil-water (HLB = 7.3) was established. The results showed that the surfactant Tween-80 with an HLB value of 15 was beneficial to increase the molar yield of 9α-OH-AD. When 4.0 g/L Tween-80 was added, the molar yield of 9α-OH-AD reached 38.5%. Then, it was found that the addition of the solubilizing agent 100.0 g/L soybean oil promoted the transformation of phytosterol to 9α-OH-AD by Mycobacterium sp. LY-1. At the same time, when 4.0 g/L Tween-80 and 100.0 g/L soybean oil were added, the molar yield of 9α-OH-AD achieved to 36.9%, and the quality concentration of 9α-OH-AD was 8.17 g/L, which was 324.1% higher the control experiment at higher phytosterols concentration of 30 g/L. The above results indicated that the oil-water conversion process system established in this study could increase the substrate concentration and improved the transformation ability of the Mycobacterium, which might lay a foundation for the industrial application.

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Last Update: 2019-03-13