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Eryxin Inhibits Estrogen Biosynthesis by Suppression of Aromatase*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 05
Page:
1-7
Research Field:
Articles
Publishing date:

Info

Title:
Eryxin Inhibits Estrogen Biosynthesis by Suppression of Aromatase*
Author(s):
Shi Nan 1 4 Shi Xiao-ke 1 4 Jovliqizi Azimova Bahtigul 1 3 Abduvaliev Anvar 2 Rakhmanov Alisher 2 & Wang Fei 1**
1 Chengdu Institute of Biology, Chinese academy of Sciences, Chengdu 610041, China
2 Tashkent Medical Academy, Tashkent 10012, Uzbekistan
3 Tashkent Pharmaceutical Institute, Tashkent 10012, Uzbekistan 4 University of Chinese Academy of Sciences, Beijing 100000, China
Keywords:
eryxin 17-estradiol aromatase ovarian granulosa cells diseases
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.01028
DocumentCode:

Abstract:
Due to the level of estrogen is closely related to women’s physiological and mental health, this paper aimed to investigated the effect of eryxin on estrogen biosynthesis. Am-blue method was used to detecte the effect of eryxin on the proliferation of human ovarian granulosa cells (KGN cells). The effects of eryxin on estrogen biosynthesis in KGN cells and aromatase-overexpressing HEK-293A (HEK-293Aaro) cells were examined by enzyme-linked immunosorbent assay (ELISA). The expression of aromatase protein in KGN cells were detected by Western blotting. We also examined the expression of aromatase gene and Promoter II by RT-qRCR. The results showed that 1-150 μg/mL of eryxin had no effect on cell proliferation but it potently inhibited the biosynthesis of 17β-estradiol in KGN cells in a dose-dependent manner, whereas it had no effect on the catalytic activity of aromatase in HEK-293Aaro cells. Eryxin significantly down-regulated the mRNA and protein expression of aromatase protein by decreasing the expression of aromatase Promoter Ⅱ. Boiling and enzymolysis significantly attenuated the inhibitory effect of eryxin on estrogen biosynthesis in KGN cells. Eryxin suppresses estrogen biosynthesis in KGN cells by the inhibition of aromatase expression, Eryxin warrants further investigation as a new candidate for the treatnment of estrogen-related diseases.

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Last Update: 2019-03-13