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Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 06
Page:
1-10
Research Field:
Articles
Publishing date:

Info

Title:
Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure*
Author(s):
YAN Xiaotao1 GUI Fang1 ZHANG Jinhua2 3 SI Jing2 ZHANG Hong2 4**
1 Lanzhou urban water supply (Group) Co., Ltd, LanZhou 730000, China
2 Institute of modern physics, Chinese academy of sciences, LanZhou 730000, China
3 University of China Academy of Sciences, BeiJing 100049, China
4 Wuwei Cancer Hospital,Wuwei 733000,China
Keywords:
lead HLY78 Wnt signaling zebrafish developmental toxicity
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2019.01004
DocumentCode:

Abstract:
The aim of this study was to investigate the role of Wnt signaling in mediating the developmental toxicity induced by lead (Pb) exposure using zebrafish embryos. Zebrafish (Danio rerio) embryos were randomly divided into 10 groups. Pb (0、6、12、24、48 μmol/L) were respectively exposure to zebrafish embryos during 4-144 h post fertilization, with or without HLY78, an activator of the Wnt signaling pathway (5 μmol/L). The expression of wnt3a was detected using Quantitative real-time PCR (qRT-PCR). Pb exposure significantly decreased the expression of wnt3a, supplementation with HLY78 was able to reduce this effect. Mortality, hatchability and malformation were observed and recorded. Spontaneous movement, heart rate and locomotive behavior in larval zebrafish were recorded and analyzed using the Vision XT software.Pb exposure significantly decreased hatchability, heart rate and swimming activities (P <0.05), meanwhile, the mortality and malformation were significantly increased (P <0.05). Compare to individual Pb exposure, supplementation with HLY78 significantly increased hatchability, heart rate and swimming activities (P <0.05), and the mortality and malformation of larvae were significantly decreased (P <0.05). However, there was no significant difference in the spontaneous movement of embryos between groups. Overall, Pb exposure induced a significant increase of developmental toxicity, and decreased the expression of wnt3a. Activation of wnt signaling allievates Pb exposure induced developmental toxicity. Results in this study allow us to further understand the molecular mechanisms of Pb-induced developmental toxicity, which potentially involves the inhibition of Wnt signaling.

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Last Update: 2019-03-28