|Table of Contents|

Diversity and community composition of phosphorus accumulating organisms (PAOs) in the sediments of the Sanshiliujiao Lake reservoir(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2018 06
Page:
1254-1262
Research Field:
Articles
Publishing date:

Info

Title:
Diversity and community composition of phosphorus accumulating organisms (PAOs) in the sediments of the Sanshiliujiao Lake reservoir
Author(s):
SHE Chenxing et al.
1 College of Environmental Science and Engineering, Fujian Normal University, Fuzhou 350007, China 2 Fujian Key Laboratory of Pollution Control and Resource Reuse, Fuzhou 350007, China
Keywords:
reservoir sediment phosphorus accumulating organisms (PAOs) diversity terminal restriction fragment length polymorphism (T-RFLP) community composition
CLC:
Q178.1
PACS:
DOI:
10.19675/j.cnki.1006-687x.2018.03001
DocumentCode:

Abstract:
The diversity and community composition of phosphorus accumulating organisms (PAOs) in the sediments of the Sanshiliujiao Lake reservoir in Pingtan island, Fujian Province, were investigated by terminal restriction fragment length polymorphism (T-RFLP) methods combined with the construction of clone libraries. The diversity of PAOs in summer samples was significantly higher than that in winter. The dominant terminal restriction fragments (T-RFs) of PAOs were in the range of 200 to 300 bp in summer, while they were in the range of 150 to 200 bp in winter. Cloning sequencing and phylogenetic analysis showed that the main phyla of PAOs in the sediment of the Sanshiliujiao Lake reservoir were Proteobacteria, Actinobacteria, and Acidobacteria. Anaeromyxobacter, Azospirillum, Microlunatus phosphovorus, and Burkholderia were the dominant genera in the summer samples. Anaeromyxobacter, Methylobacterium, Solibacter, Azospirillum, and Microlunatus phosphovorus were the dominant genera in sediment samples in winter. The results of this study demonstrated that the diversity and community composition of PAOs in the sediments of the Sanshiliujiao Lake reservoir had specific seasonal characteristics. Various forms of phosphorus also showed their influence on the diversity of PAOs, especially Fe/Al-P. Our findings lays a scientific foundation for the future disclosure of microbial mechanisms involved in the phosphorus metabolic cycle in the sediments of reservoirs.

References

1. Skennerton CT, Barr JJ, Slater FR, Bond PL, Tyson GW. Expanding our view of genomic diversity in Candidatus Accumulibacter clades [J]. Environ Microbiol, 2015, 17 (5): 1574-1585
2. Stokholmbjerregaard M, Mcilroy SJ, Nierychlo M, Karst SM, Albertsen M, Nielsen PH. A critical assessment of the microorganisms proposed to be important to enhanced biological phosphorus removal in full-scale wastewater treatment systems [J]. Front Microbiol, 2017, 8: doi 10.3389/fmicb.2017.00718
3. Wong PY, Cheng KY, Kaksonen AH, Sutton DC, Ginige MP. A novel post denitrification configuration for phosphorus recovery using polyphosphate accumulating organisms [J]. Water Res, 2013, 47 (17): 6488-6495
4. Weissbrodt DG, Holliger C, Morgenroth E. Modeling hydraulic transport and anaerobic uptake by PAOs and GAOs during wastewater feeding in EBPR granular sludge reactors [J]. Biotechnol Bioeng, 2017, 114 (8): 1688-1702
5. Chan C, Guisasola A, Baeza JA. Enhanced biological phosphorus removal at low sludge retention time in view of its integration in a-stage systems [J]. Water Res, 2017, 118: 217-226
6. Valverdepérez B, Wágner DS, Lóránt B, Gülay A, Smets BF, Plósz BG. Short-sludge age ebpr process-microbial and biochemical process characterisation during reactor start-up and operation [J]. Water Res, 2016, 104: 320-329
7. 陈楠. 太湖沉积物微生物群落组成与物质循环及藻华爆发的相关性[D]. 北京: 中国农业大学, 2015 [Chen N. Relationship between microbial community structure and nutrient cycles and algal bloom in Taihu Lake [D]. Beijing: China Agricultural University, 2015]
8. 胡颖, 江和龙, 何超群. 太湖草藻型沉积物的物理化学特征及其对沉积物中聚磷菌的影响[J]. 生态环境学报, 2012, 21 (11): 1859-1864 [Hu Y, Jiang HL, He CQ. Characteristic of physics and chemistry of grass/algae type zone sediments in Lake Taihu and effect on polyphosphate accumulation [J]. Acta Ecol Environ Sin, 2012, 21 (11): 1859-1864]
9. Hupfer M, Gloess S, Grossart HP. Polyphosphate-accumulating microorganisms in aquatic sediments [J]. Aquat Microb Ecol, 2007, 47 (3): 299-311
10. García Martín H, Ivanova N, Kunin V, Warnecke F, Barry KW, McHardy AC, Yeates C, He S, Salamov AA, Szeto E, Dalin E, Putnam NH, Shapiro HJ, Pangilinan JL, Rigoutsos I, Kyrpides NC, Blackall LL, McMahon KD, Hugenholtz P. Metagenomic of two enhanced biological phosphorus removal (EBPR) sludge communities [J]. Nat Biotechnol, 2006, 24 (10): 1269-1263
11. Camejo PY, Owen BR, Martirano J, Ma J, Kapoor V, Domingo JS, McMahon KD, Noguera DR. Candidatus Accumulibacter phosphatis clades enriched under cyclic anaerobic and microaerobic conditions simultaneously use different electron acceptors [J]. Water Res, 2016, 102: 125-137
12. 张丽敏, 曾薇, 王安其, 张洁, 彭永臻. 城市污水处理厂Candidatus Accumulibacter的菌群结构及定量分析[J]. 环境科学学报, 2016, 36 (4): 1226-1235 [Zhang LM, Zeng W, Wang AQ, Zhang J, Peng YZ. Community structures and quantitative analyses of Candidatus Accumulibacter in municipal wastewater treatment plants [J]. Acta Sci Circumstant, 2016, 36 (4): 1226-123]
13. 东野脉兴, 樊竹青, 张灼, 夏学惠, 田升平, 周建民. 滇池微生物解磷与聚磷作用的实验研究及磷的现代沉积与微生物成矿作用[J]. 吉林大学学报, 2003, 33 (3): 282-289 [Dongye MX, Fan ZQ, Zhang Z, Xia XH, Tian SP, Zhou JM. Experimental reseaches on phosphorus decomposing and concentrating of microorganism in Dianchi lake: an example of modern phosphorus deposition and microorganism mineralization [J]. J Jilin Univ (Earth Sci Ed), 2003, 33 (3): 282-289]
14. 夏学惠, 东野脉兴, 周建民, 田升平, 张灼, 彭彦华. 滇池现代沉积物中磷的地球化学及其对环境影响[J]. 沉积学报, 2002, 20 (3): 416-420 [Xia XH, Dongye MX, Zhou JM, Tian SP, Zhang Z, Peng YH. Geochemistry and influence to environment of phosphorus in modern sediment in Dianchi Lake [J]. Acta Sedimentol Sin, 2002, 20 (3): 416-420]
15. He S, Gall DL, McMahon KD. Candidatus Accumulibacter population structure in enhanced biological phosphorus removal sludges as revealed by polyphosphate kinase genes [J]. Appl Environ Microbiol, 2007, 73 (18): 5865-5874
16. Kunin V, He S, Warnecke F, Peterson SB, Martin HG, Haynes M, Ivanova N, Blackall LL, Breitbart M, Rohwer F, McMahon KD, Hugenholtz P. A bacterial metapopulation adapts locall to phase predation despite global dispersal [J]. Genome Res, 2008, 18 (2): 293-297
17. 朱艳霞. 太湖入水口底泥微生物宏基因组及聚磷菌多样性研究[D]. 苏州: 苏州科技学院, 2012 [Zhu YX. Research an metagenomie DNA and biodiversity of phosphorus-accumulating organisms in the sediments from water inlet of Taihu Lake [D]. Suzhou: Suzhou University of Science and Technology, 2012]
18. 翁彩云. 平潭县三十六脚湖水环境问题及周边工程治理对策浅析[J]. 能源与环境, 2009 (3): 11-13 [Weng CY. Analyses on water environment problems and engineering treating counter measure of surrounding in Sanshiliujiao Lake in Pingtan County [J]. Energy Environ, 2009 (3): 11-13]
19. Ruban V, López-Sánchez JF, Pardo P, Rauret G, Muntau H, Quevauviller P. Harmonized protocol and certified reference material for the determination of extractable contents of phosphorus in freshwater sediments-a synthesis of recent works [J]. Fresenius J Anal Chem, 2001, 370 (2-3): 224-228
20. 苏玉萍, 林佳, 何灵, 林婉珍, 王家乐. 福建省山仔水库沉积物磷对水体磷浓度贡献的估算[J]. 湖泊科学, 2008, 20 (6): 748-754 [Su YP, Lin J, He L, Lin WZ, Wang JL. Calculation of the contribution of the sediment phosphorus to the phosphorus concentration of the water body of Shanzi deep reservoir in Fujian Province [J]. J Lake Sci, 2008, 20 (6): 748-754]
21. Ludwig F, Mehlig L, Eschenhagen M, R?ske I. Development of new primer systems for the detection of the polyphosphate kinase gene in activated sludge [J] . Water Sci and Technol, 2009, 30 (2): 213-218
22. Fierer N, Schimel JP, Holden PA. Influence of drying-rewetting frequency on soil bacterial community structure [J]. Microb Ecolol, 2003, 45 (1): 63-71
23. Manzano M, Moran AC, Tesser B, Gonzalez B. Role of eukaryotic microbiota in soil survival and catabolic Performance of the 2,4-D herbicide degrading bacteria Cupriavidus necator JMP134 [J]. Antonie van Leeuwenhoek, 2007, 91 (2): 115-126
24. 尹承苗, 王功帅, 李园园, 陈学森, 吴树敬, 毛志泉. 连作苹果园土壤真菌的T-RFLP分析[J]. 生态学报, 2014, 34 (4): 837-846 [Yin CM, Wang GS, Li YY, Chen XS, Wu SJ, Mao ZQ. Assessment of fungal diversity in apple replanted orchard soils by T-RFLP analysis [J]. Acta Ecol Sin, 2014, 34 (4): 837-846]
25. 佘晨兴, 仝川. 闽江口芦苇沼泽湿地土壤产甲烷菌群落结构的垂直分布[J]. 生态学报, 2012, 32 (17): 5299-5308 [She CX, Tong C. Vertical distribution of methanogen community structures in Phragmites australis marsh soil in the Min River estuary [J]. Acta Ecol Sin, 2012, 32 (17): 5299-5308]
26. 佘晨兴, 仝川, 王维奇. 互花米草沼泽湿地产甲烷古菌的多样性及垂向分布[J]. 环境科学学报, 2014, 34 (1): 186-193 [She CX, Tong C, Wang WQ. Vertical distribution and diversity of methanogenic archaea in estuarine marsh dominated by Spartina alterniflora [J]. Acta Sci Circumstant, 2014, 34 (1): 186-193]
27. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0 [J]. Mol Biol Evol, 2007, 24 (8): 1596-1599
28. 伍思宇, 周志如, 尤青, 张文飞, 徐达, 李特伦, 姚琦, 张起畅, 吴红萍, 王锐萍. 海南东寨港红树林聚磷菌的筛选及其特性[J]. 应用与环境生物学报, 2016, 22 (3): 397-401 [Wu SY, Zhou ZR, You Q, Zhang WF, Xu D, Li TL, Yao Q, Zhang QC, Wu HP, Wang RP. Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China [J]. Chin J Appl Environ Biol, 2016, 22 (3): 397-401]
29. 王亚东, 王少坡, 郑莎莎, 张燕, 孙力平, 杜金山. 生物除磷系统的聚磷微生物种群及其检测方法[J]. 环境工程, 2015, 33 (2): 21-26 [Wang YD, Wang SP, Zheng SS, Zhang Y, Sun LP, Du JS. Poly-P accumulating microorganisms and identifying methods for biological phosphorus removal system [J]. Environ Eng, 2015, 33 (2): 21-26]
30. 魏儒平, 闫诚, 杨欣妍, 何晓云, 王鑫, 杨柳燕. 强化生物除磷系统的功能微生物研究进展[J]. 生物技术通报, 2017, 33 (10): 1-8 [Wei RP, Yan C, Yang XY, He XY, Wang X, Yang LY. Research progress on the functional microorganisms in enhanced biological phosphorus removal (EBPR) systems [J]. Biotechnol Bull, 2017, 33 (10): 1-8]
31. 吴晓娜, 王助贫, 谢恩, 任梦丽, 刘婷婷, 郑蕾, 丁爱中. 一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J]. 环境工程学报, 2018, 12 (2): 544-551 [Wu XN, Wang ZP, Xie E, Ren ML, LIU TT, Zheng L, Ding AZ. Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal [J]. Chin J Environ Eng, 2018, 12 (2): 544-551]
32. Mcmahon KD, Read EK. Microbial contributions to phosphorus cycling in eutrophic lakes and wastewater [J]. Annu Rev Microbiol, 2013, 67 (67): 199-219
33. Nielsen PH, Saunders AM, Hansen AA, Larsen P, Nielsen JL. Microbial communities involved in enhanced biological phosphorus removal from wastewater--a model system in environmental biotechnology [J]. Curr Opin Biotechnol, 2012, 23 (3): 452-459
34. Peterson SB, Warnecke F, Madejska J, Mcmahon KD, Hugenholtz P. Environmental distribution and population biology of Candidatus Accumulibacter, a primary agent of biological phosphorus removal [J]. Environ Microbiol, 2008, 10 (10): 2692-2703
35. Hideyuki T, Yuji S, Satoshi H. Comparative analysis of bacterialdiversity in fresh water sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques [J]. Appl Environ Microbiol, 2005, 71 (4): 2162-2169
36. Schwarz JIK, Eckert W, Conrad R. Community structure of Archaea and bacteria in a profundal lake sediment Lake Kinneret [J]. Syst Appl Microbiol, 2007, 30 (3): 239-254
37. 胡颖, 王世和, 江和龙, 何超群. 碳源对太湖沉积物中聚磷菌的影响[J]. 安全与环境工程, 2011, 18 (6): 49-52 [Hu Y, Wang SH, Jiang HL, He CQ. Effect of different carbon resources on the enrichment of polyphosphate accumulating organisms in sediments from Taihu Lake [J]. Saf Environ Eng, 2011, 18 (6): 49-52]
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Last Update: 2018-12-25