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Sodium dodecyl sulfate degradation performance of Pseudomonas sp. SDS-2 and its activity regulation at low temperatures(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 01
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Sodium dodecyl sulfate degradation performance of Pseudomonas sp. SDS-2 and its activity regulation at low temperatures
CHEN Yangwu1 2 3 ZHANG Ruomu1 2 DAI Fazhi1 2 LI Xudong1 2 & TAN Zhouliang1 2**
1 Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 3 University of Chinese Academy of Sciences, Beijing 100049, China
sodium dodecyl sulfate (SDS) Pseudomonas sp. SDS-2 low temperature acclimation regulation

Biological methods have been widely used for surfactant removal; however, low temperatures always exert adverse effects on the metabolic activity of microorganisms. In this study, we investigated the optimum growth conditions for a sodium dodecyl sulfate (SDS)-degrading strain and evaluated its low-temperature degradation activity with different regulation strategies. The SDS-degrading strain screened in this study was identified using the 16S rRNA gene sequencing method. The degradation performance of the strain was evaluated under different temperatures, pH values, substrate concentrations, and inoculum sizes. Furthermore, the metabolic activities of the strain under different regulation strategies (acclimation and extra substance addition) at low temperatures were indirectly evaluated by chemical oxygen demand (COD) removal efficiency. The SDS-degrading strain was isolated and identified as Pseudomonas sp. SDS-2. The optimum growth conditions for the SDS-2 strain were 30 ℃, pH 9, and 120 mg/L ammonia concentration. The inoculum size showed no obvious effects on SDS degradation. The COD removal rate was as high as 355.3 mg L-1 h-1 when the initial SDS concentration reached 2 500 mg/L. After long-term acclimation at 15 ℃, the substrate removal efficiency at 15 ℃ reached the same level as that at 30 ℃. At 10 ℃, the substrate removal efficiencies were increased by 25.3% and 24.6% at 48 h with sodium succinate and potassium nitrate addition, respectively. Furthermore, the substrate removal increased by 22.8% and 11.5% at 24 h with the addition of peptone and multi-vitamins, respectively. The high SDS-degrading activity of the strain SDS-2 shows that it can be used as a microbial resource for actual SDS-containing wastewater treatment, and the regulation strategies applied in this study can be used as potential methods for SDS remediation at low temperatures.


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Last Update: 2019-02-25