|Table of Contents|

Heterologous overexpression of Populus euphratica BAK1;1 gene enhanced resistance to Pst DC3000 in tobacco(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2018 02
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Heterologous overexpression of Populus euphratica BAK1;1 gene enhanced resistance to Pst DC3000 in tobacco
TANG Hai YAO Yin′an ZHANG Guoyan LI Xing DING Hongxia WU Yingqing GAO Yongfeng
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
PeBAK11 Populus euphratica tobacco pathogenesis-related genes Pst DC3000
S5.034 : Q786

BRI1-ASSOCIATED RECEPTORKINASE1 (BAK1), a leucine-rich repeat (LRR) receptor protein kinase, plays a significant role in brassinosteroid (BR) signaling. Furthermore, it combines with other LRR-RLKs protein to initiate immune response in plants. The objective of this study was to (1) investigate the function of the Populus euphratica BAK1;1 gene in the resistance of transgenic tobacco to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and (2) discuss the regulation pathway of PeBAK1;1 involved in the resistance to plant pathogen. We cloned the cDNA sequence of the P. euphratica PeBAK1;1 gene, constructed the pBI121-35S::PeBAK1;1 over-expression vector, and then transformed it into wild-type tobacco by Agrobacterium-mediated transformation to obtain PeBAK1;1-overexpressing transgenic tobacco plants. The bioinformatic analysis showed that the PeBAK1;1 protein contained all the structural features of the plant SERK family. The phylogenetic tree showed that PeBAK1;1 has the highest sequence homology with PtBAK1. The gene expression profile results indicated that the expression of PeBAK1;1 in the root was higher than that in the leaf and stem. The wild-type tobacco plants showed an obvious susceptibility to Pst DC3000, whereas the transgenic plants exhibited enhanced resistance to Pst DC3000. Compared with that of the wild-type (WT), the real-time PCR and quantitative real-time PCR analysis revealed that the expression of pathogenesis-related genes (including PR1, PR3, PR4, and PR5), BAK1-interacting receptor kinase 1 gene, and BONZAI1 gene was upregulated in 35S::PeBAK1;1 transgenic tobacco plants. In conclusion, the PeBAK1;1 gene plays a positive regulatory role in 35S::PeBAK1;1 transgenic tobacco against Pst DC3000, which can enhance the resistance of plants to pathogen.


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Last Update: 2018-04-25