|Table of Contents|

A novel gene SI (stress insensitive) is involved in the response to abiotic stress in Arabidopsis(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2018 02
Page:
328-334
Research Field:
Articles
Publishing date:

Info

Title:
A novel gene SI (stress insensitive) is involved in the response to abiotic stress in Arabidopsis
Author(s):
ZOU Ting LIU Huanhuan LIU Yongsheng WANG Songhu
1Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China3 Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Nay Pyi Taw 05282, Myanmar
Keywords:
Arabidopsis DUF1336 transient expression abscisic acid (ABA) abiotic stress
CLC:
Q945.78 : Q786
PACS:
DOI:
10.19675/j.cnki.1006-687x.2017.05043
DocumentCode:

Abstract:
Abiotic stresses are a major cause of loss of crop yield. The adaptation of plants to abiotic stress has been always a hotspot of botanical research. In this study, we cloned and characterized a novel gene SI (stress insensitive) involved in the response to abiotic stress in Arabidopsis. The SI protein has a new domain called DUF1336, and this family represents the C-terminus (approximately 250 residues) of a number of hypothetical plant proteins of unknown function. Furthermore, the SI protein is conserved among monocots and dicots. The subcellular localization analysis showed that the SI protein is localized in the plasma membrane. The quantitative RT-PCR analysis showed that the SI gene is constitutively expressed in various tissues. The expression of SI was relatively high in flowers, siliques, and seeds. Abiotic stress and abscisic acid (ABA) treatment can enhance the expression of SI gene, and SI transcripts were upregulated under ABA, NaCl, and cold treatments by 3.5, 2.1, and 4.7 folds, respectively. The T-DNA insertion mutant of SI gene (SALK 021811C) showed increased tolerance to cold and salt stresses. In addition, the seed germination experiments indicated that the seeds of SI mutant were insensitive to salt stress and ABA treatment. In conclusion, the results demonstrate that the SI gene plays a negative role in the tolerance of plants to abiotic stress and is a good candidate gene for genetic engineering to increase the tolerance of crop to abiotic stress.

References

1 Barrero-Gil J, Salinas J. CBFs at the crossroads of plant hormone signaling in cold stress response [J]. Mol Plant, 2017, 10: 542-544
2 Byun MY, Lee J, Cui LH, Kang Y, Oh TK, Park H, Lee H, Kim WT. Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants [J]. Plant Sci, 2015, 236: 61-74
3 Kashyap P, Deswal R. A novel class I Chitinase from Hippophae rhamnoides: indications for participating in ICE-CBF cold stress signaling pathway [J]. Plant Sci, 2017, 259: 62-70
4 Marozsán-Tóth Z, Vashegyi I, Galiba G, Tóth B. The cold response of CBF genes in barley is regulated by distinct signaling mechanisms [J]. J Plant Physiol, 2015, 181: 42-49
5 Medina J, Catalá R, Salinas J. The CBFs: three Arabidopsis transcription factors to cold acclimate [J]. Plant Sci, 2011, 180: 3-11
6 Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S. The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis [J]. New Phytol, 2016, 212: 345-353
7 Ding Y, Li H, Zhang X, Xie Q, Gong Z, Yang S. OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis [J]. Dev Cell, 2015, 32: 278-289
8 Li H, Ye K, Shi Y, Cheng J, Zhang X, Yang S. BZR1 positively regulates freezing tolerance via CBF-dependent and CBF-independent pathways in Arabidopsis [J]. Mol Plant, 2017, 10: 545-559
9 Zhang Z, Li J, Pan Y, Li J, zhou L, Shi H, Zeng Y, Guo H, Yang S, Zheng W, Yu J, Sun X, Li G, Ding Y, Ma L, Shen S, Dai L, Zhang H, Yang S, Guo Y, Li Z. Natural variation in CTB4a enhances rice adaptation to cold habitats [J]. Nature Commun, 2017, 8: 14788
10 Zhu JK. Salt and drought stress signal transduction in plants [J]. Annu Rev Plant Biol, 2002, 53: 247-273
11 Rivero RM, Kojima M, Gepstein A, Sakakibara H, Mittler R, Gepstein S, Blumwald E. Delayed leaf senescence induces extreme drought tolerance in a flowering plant [J]. PNAS, 2007, 104: 19631-19636
12 Wang SH, Blumwald E. Salt-induced chloroplast degradation in Arabidopsis is regulated via a process independent of autophagy and senescence-associated vacuoles [J]. Plant Cell, 2014, 26: 4875-4888
13 Danquah A, de Zélicourt A, Boudsocq M, Neubauer J, Frei dit Frey N, Leonhardt N, Pateyron S, Gwinner F, Tamby J-P, Ortiz-Masia D, Marcote MJ, Hirt H, Colcombet J. Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana [J]. Plant J, 2015, 82: 232-244
14 Ding ZJ, Yan JY, Li CX, Li GX, Wu YR, Zheng SJ. Transcription factor WRKY46 modulates the development of Arabidopsis lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis [J]. Plant J, 2015, 84: 56-69
15 Lee HG, Seo PJ. The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis [J]. Plant J, 2015, 82: 962-977
16 Lv P, Zhang C, Liu J, Liu X, Jiang G, Jiang X, Khan MA, Wang L, Hong B, Gao J. RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose (Rosa hybrida) petal senescence [J]. Plant J, 2014, 78: 578-590
17 Magnan F, Ranty Bt, Charpenteau M, Sotta B, Galaud J-P, Aldon D. Mutations in AtCML9, a calmodulin-like protein from Arabidopsis thaliana, alter plant responses to abiotic stress and abscisic acid [J]. Plant J, 2008, 56: 575-589
18 Shu K, Chen Q, Wu Y, Liu R, Zhang H, Wang P, Li Y, Wang S, Tang S, Liu C, Yang W, Cao X, Serino G, Xie Q. ABI4 mediates antagonistic effects of abscisic acid and gibberellins at transcript and protein levels [J]. Plant, 2016, 85: 348-361
19 Fang Q, Jiang,TZ Xub LX, Liu H, Mao H, Wang XQ, Jiao B, Duan Yj, Wang Q, Dong QN, Yang L, Tian GZ, Zhang C, Zhou Y. A salt-stress-regulator from the Poplar R2R3 MYB family integrates the regulation of lateral root emergence and ABA signaling to mediate salt stress tolerance in Arabidopsis [J]. Plant Physiol Biochem, 2017, 114: 100-110
20 Barrero-Gil J, Salinas J. CBFs at the crossroads of plant hormone signaling in cold stress response [J]. Mol Plant, 2017, 10: 542-544
21 Choi SW, Lee SB, Na YJ, Jeung SG, Kim SY. Arabidopsis MAP3K16 and other salt-inducible MAP3Ks regulate ABA response redundantly [J]. Mol Cells, 2017, 40 (3): 230-242
22 Ding Y, Li H, Zhang X, Xie Q, Gong Z, Yang S. OST1 Kinase modulates freezing tolerance by enhancing ice1 stability in Arabidopsis [J]. Dev Cell, 2015, 32: 278-289
23 He L, Shi X, Wang Y, Guo Y, Yang K, Wang Y. Arabidopsis ANAC069 binds to C[A/G]CG[T/G] sequences to negatively regulate salt and osmotic stress tolerance [J]. Plant Mol Biol, 2017, 93: 369-387
24 Danquah A, de Zélicourt A, Boudsocq M, Neubauer J, Frei dit Frey N, Leonhardt N, Pateyron S, Gwinner F, Tamby J-P, Ortiz-Masia D, Marcote MJ, Hirt H, Colcombet J. Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana [J]. J, 2015, 82: 232-244
25 Shu K, Chen Q, Wu Y, Liu R, Zhang H, Wang P, Li Y, Wang S, Tang S, Liu C, Yang W, Cao X, Serino G, Xie Q. ABI4 mediates antagonistic effects of abscisic acid and gibberellins at transcript and protein levels [J]. Plant J, 2015, 85: 348-361
26 Tang DZ, Ade J, Frye CA, Innes RW. Regulation of plant defense responses in Arabidopsis by EDR2, a PH and START domain-containing protein [J]. Plant J, 2005, 44 (2): 245–257
27 Satheesh V, Chidambaranathan P, Jagannadham PT, Kumar V, Jain PK, Chinnusamy V, Bhat SR, Srinivasan R. Transmembrane START domain proteins: in silico identification, characterization and expression analysis under stress conditions in chickpea (Cicer arietinum L.) [J]. Plant Signalling Behav, 2016, 11 (2): 1559-2324
28 Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR. Genome-wide insertional mutagenesis of Arabidopsis thaliana [J]. Science, 2003, 301: 653-657
29 Li YX, Deng H, Miao M, Li HR, Huang SX, Wang SH, Liu YS. Tomato MBD5, a methyl CpG binding domain protein, physically interacting with UV-damaged DNA binding protein-1, functions in multiple processes [J]. New Phytol, 2016, 210: 208-226
30 Xiao J, Li CH, Xu SJ, Xing LJ, Xu YY, Chong K. JACALIN-LECTIN LIKE1 regulates the nuclear accumulation of GLYCINE-RICH RNA-BINDING PROTINE7, influencing the RNA processing of FLOWERING LOCUS C antisense transcripts and flowering time in Arabidopsis [J]. Plant Physiol, 2015, 169: 2102-2117
31 Yoo S-D, Cho Y-H, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis [J]. Nature Prot, 2007, 2: 1565-1572
32 Liu Z, Jia Y, Ding Y, Shi Y, Li Z, Guo Y, Gong Z, Yang S. Plasma membrane CRPK1-mediated phosphorylation of 14-3-3 proteins induces their nuclear import to fine-tune CBF signaling during cold response [J]. Mol Cell, 2017, 66: 117-128
33 ?rvar BL, Sangwan V, Omann F, Dhindsa RS. Early steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity [J]. Plant J, 2000, 23: 785-794
34 Sun J, Chen S, Dai S, Wang R, Li N, Shen X, Zhou X, Lu C, Zheng X, Hu Z, Zhang Z, Song J, Xu Y. NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species [J]. Plant Physiol, 2008, 149: 1141-1153
35 Zhao F, Song CP, He J, Zhu H. Polyamines improve K+/Na+ homeostasis in barley seedlings by regulating root ion channel activities [J]. Plant Physiol, 2007, 145: 1061-1072

Memo

Memo:
-
Last Update: 2018-04-25