|Table of Contents|

Inhibition mechanism of fermentation broth extract of Phomopsis sp. strain S4 on cell membranes of Magnaporthe oryzae(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2018 02
Page:
342-346
Research Field:
Articles
Publishing date:

Info

Title:
Inhibition mechanism of fermentation broth extract of Phomopsis sp. strain S4 on cell membranes of Magnaporthe oryzae
Author(s):
LI Furong;  DING Tao ;  BAI Linhan
Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Keywords:
endophytic fungus;  Phomopsis;  Magnaporthe oryzae;  antifungal effect;  cell membrane;  ergosterol
CLC:
Q939.95
PACS:
DOI:
10.19675/j.cnki.1006-687x.2017.04013
DocumentCode:

Abstract:
The secondary metabolites of endophytic Phomopsis sp. strain S4 show antifungal activity against a variety of plant pathogens, which implies that strain S4 has potential prospect in crop disease control. The aim of this study was to explore the inhibition mechanisms of S4 against plant pathogenic fungi. Magnaporthe oryzae was used as the main pathogenic material for the research. Cell membrane changes were detected using antifungal experiments, scanning electron microscopy, Q RT-PCR, and cell content leaking experiments. The results of scanning electron microscopy showed that M. oryzae mycelia, after treatment with S4 fermented product extract, decreased in size, suggesting the integrity of the cell membrane was destroyed. The genes related to ergosterol synthesis, which plays an important role in membrane integrity, were studied though Q RT-PCR. The results showed that the expression levels of ERG1, ERG11, and ERG6 genes were down-regulated by 2.0, 1.40, and 2.7-fold, respectively, whereas that of ERG7 was up-regulated by 1.38-fold, which means the ergosterol synthesis pathway was destroyed. The physiological experiments also showed that the cell contents of M. oryzae mycelia treated with S4 fermented product extract leaked significantly, which was consistent with the Q RT-PCR results. The results showed that S4 fermentation broth exact could destroy the cell membrane integrity by inhibiting ergosterol synthesis, and eventually inhibit M. oryzae cell growth.

References

1. Talbot NJ. On the trail of a cereal killer: exploring the biology of Magnaporthe grisea [J]. Annu Rev Microbiol, 2003, 57 (1): 177-202
2. Khush GS, Jena KK. Current status and future prospects for research on blast resistance in rice (Oryza sativa L.) [M]. Suwon: Springer Netherlands, 2009: 1-10
3. Qi G, Zhu F, Du P, Yang X, Qiu D, Yu Z, Chen J, Zhao X. Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway [J]. Peptides, 2010, 31 (11): 1978-1986
4. Strange RN, Scott PR. Plant disease: a threat to global food security [J]. Annu Rev Phytopathol, 2005, 43 (1): 83-116
5. Burden RS, Cooke DT, Carter GA. ChemInform abstract: inhibitors of sterol biosynthesis and growth in plants and fungi [J]. Phytochemistry, 1989, 28 (7): 1791-1804
6. Iglesias M, Ballesta JP. Mechanism of resistance to the antibiotic trichothecin in the producing fungi [J]. Eur J Biochem, 1994, 223 (2): 447-453
7. 刁春玲, 刘芳, 宋宝安. 农用杀菌剂作用机理的研究进展[J]. 农药, 2006, 45 (6): 374-377 [Diao CL, Liu F, Song BA. Research progress on mechanism of agricultural fungicide [J]. Pesticide, 2006, 45 (6): 374-377]
8. Zhao XR, Lin Q, Brookes PC. Does soil ergosterol concentration provide a reliable estimate of soil fungal biomass? [J]. Soil Biol Biochem, 2005, 37 (2): 311-317
9. Lu FT, Ma DC, Yan W, Guo J, Bai LH. The antitumor activity research of 3-NPA from a Phomopsis sp. and the fermentation condition optimization [J]. Lett Appl Microbiol, 2015, 61 (2): 165-170
10. 郭静, 麻栋策, 黄丽杰, 卢菲婷, 白林含. 内生真菌源3-硝基丙酸的分离鉴定及其抗菌作用的研究[J]. 四川大学学报(自然科学版), 2015, 52 (2): 429-434 [Guo J, Ma DC, Huang LJ, Lu FT, Bai LH. Isolation and identification of 3-nitro propionic acid as an antibacterial agent from an endophytic fungus [J]. J Sichuan Univ (Nat Sci Ed), 2015, 52 (2): 429-434]
11. 黄丽杰, 李芙蓉, 杨志荣, 白林含. 拟茎点霉属S4菌株发酵产物对菌核病的防治作用[J]. 应用与环境生物学报, 2015, 21 (6): 1090-1094 [Huang LJ, Li FR, Yang ZR, Bai LH. Biocontrol effcacy of Phomopsis sp. strain S4 against Sclerotinia sclerotiorum [J]. Chin J Appl Environ Biol, 2015, 21 (6): 1090-1094]
12. 陈琴, 刘庆亚, 陈次琼, 刘慧美, 龙章富. 郁金茎叶提取物抑杀植物病原真菌活性研究与GC/MS分析[J]. 四川大学学报(自然科学版), 2017, 54 (1): 209-214 [Chen Q, Liu QY, Chen CQ, Liu HM, Long AF. Antifungal activity and GC/MS analysis of extracts from Curcuma phaeocaulis stems and leaves against plant pathogenic fungi [J]. J Sichuan Univ (Nat Sci Ed), 2017, 54 (1): 209-214]
13. Mercer EI. Sterol biosynthesis inhibitors: their current status and modes of action [J]. Lipids, 1991, 26 (8): 584-597
14. 沙家骏. 国外新农药品种手册[M]. 北京: 化学工业出版社, 1992 [Sha JJ. Manual of foreign novel pesticide varieties [M]. Beijing: Chemical Industry Press, 1992]
15. Moebius FF, Bermoser K, Reiter RJ, Hanner M, Glossmann H. Yeast sterol C8-C7 isomerase: identification and characterization of a high-affinity binding site for enzyme inhibitors [J]. Biochemistry, 1996, 35 (51): 16871-16878
16. Kerkenaar A. Inhibition of the sterol delta 14-reductase and delta 8-delta 7-isomerase in fungi [J]. Biochem Soc Trans, 1990, 18 (1): 59-61

Memo

Memo:
-
Last Update: 2018-04-25