|Table of Contents|

Physiological mechanism of enhanced salt stress tolerance in sweet potato (Ipomoes batats L.) with overexpression of IbOr gene(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2017 01
Page:
54-59
Research Field:
Articles
Publishing date:

Info

Title:
Physiological mechanism of enhanced salt stress tolerance in sweet potato (Ipomoes batats L.) with overexpression of IbOr gene
Author(s):
zhang lijuan1 2 Li hongbing2** sun zhenmei3 wang shiwen2 Kwak Sangsoo4 & Deng xiping2**
1College of Life Science, Northwest Agriculture and Forestry University, Yangling 712100, China 2State Key Laboratory of Soil Erosion and Dry land Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest Agriculture and Forestry University, Yangling 712100, China 3Academy of Forestry, Northwest Agriculture and Forestry University, Yangling 712100, China 4Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea
Keywords:
transgenic sweet potato salt stress net photosynthetic rate transpiration rate carotenoids antioxidant enzyme
CLC:
Q78 : S531.01
PACS:
DOI:
10.3724/SP.J.1145.2016.05005
DocumentCode:

Abstract:
Sweet potato is one of the world’s most important food, feed, industrial materials and bioenergy crops. Comprehensive understanding of the physiological mechanism of whether and how transgenic sweet potato overexpressing IbOr genes enhance the tolerance to salt stress and developing a strong salt tolerant sweet potato has important theoretical and practical significance for effective utilization of saline land and alleviation of the energy crisis. Transgenic (TS) and non- transgenic (NS) sweet potato plants were treated with 150 mmol/L NaCl salt stress. The changes of photosynthetic parameters and the activities of antioxidant enzyme were studied at different times after salinity treatment. With salt stress time extended, chlorophyll and carotenoids content, photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr) all decreased evidently in both TS and NS sweet potato groups, though only slightly in TS lines. After three days of salt stress, the contents of O2—·and MDA in TS were 61.23 ?g/g FW and 22.51 ?mol/g FW, respectively. While, the contents of O2—· and MDA in NS reached 80.56 ?g/g FW and 31.92 ?mol/g FW. Compared with the NS, TS plants had lower level of O2—· and malondialdehyde (MDA) contents. After salinity treatment, the activities of SOD, POD and CAT all firstly increased, and then decreased slightly, with significantly higher enzyme activities in TS lines than in NT plants. After nine days of salt stress, the Na+ contents in TS and NS reached 25.44 mg/g DW and 35.08 mg/g DW, being 11.47 and 14.83 times of that before treatment, respectively. The Na+ content increased evidently in both but signific