|Table of Contents|

Effects of endophyte infection on reactive oxygen content and protective enzyme activity in branchlet of Casuarina equisetifolia seedling under stress of two allelochemicals*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 03
Research Field:
Publishing date:


Effects of endophyte infection on reactive oxygen content and protective enzyme activity in branchlet of Casuarina equisetifolia seedling under stress of two allelochemicals*
LONG Feng1 HONG Tao1 LIN Yongming1 XIE Anqiang1 WU Chengzhen12 HONG Wei1 & LI Jian1**
1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2College of Ecology and Resource Engineering, Wuyi University, Nanping 354300, China
allelochemical stress endophyte Casuarina equisetifolia Forst. reactive oxygen content protective enzyme activity
Q945.78 : Q948.122.3

This research aimed to explore the possibility of using endophytic fungi-Casuarina equisetifolia Forst. seedling symbiont to alleviate the continuous?planting?obstacles. It was based on the previous research on the effect of two allelochemicals (quercetin-3-α-araboside, Q3A; quercetin-3-β-glucoside, Q3B) on the reactive oxygen contents and activities of scavenging enzymes in branchlets of Casuarina equisetifolia Forst. seedlings free (EF) from endophyte (Aspergillus sp.). We studied the effect of Q3A and Q3B on the reactive oxygen contents and activities of scavenging enzymes in branchlets of Casuarina equisetifolia Forst. Seedlings infected with (EI) endophyte (Aspergillus sp.) by addition of allelochemicals to the water (of seven concentrations: 0, 12.5, 25, 50, 100, 200 and 400 mg/L) for 0-72 h to analyse the effect of endophyte infection. The results showed that the endophytic fungus colonization alleviated the allelochemical stress intensity to some degree and the effect was obvious in slight allelochemical stress at the early stage (100 mg/L 0-12 h, 50 mg/L 0-24 h, 25 mg/L 0-36 h, 12.5 mg/L 0-48 h), mainly in the reactive oxygen and its scavenging system; endophyte infection significantly reduced the production of hydrogen peroxide (H2O2), malondlaldehyde (MDA) and superoxide radical (O2-). At the same time, endophyte infection significantly improved the activity of protective enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT). But the effective concentration and stress time was in accordance with those that Casuarina equisetifolia Forst. EF endured, which showed that the protective enzyme system of C. equisetifolia. EI which was improved by endophytic fungus needed to be built before protective enzyme system synthesis and degradation suffered from lethal harms. EI had higher activities of protective enzyme activity under Q3A stress than under Q3B stress. The results of our experiment indicated that endophyte infection is beneficial to host growth under Q3A stress.


1 Mantz G, Ronco L, Monaco C. First record in Argentina of powdery mildew of Casuarina cunnighamiana caused by Oidiumsp [J]. Plant Pathol J, 2008, 90 (2): 397 2 Tomar OS, Minhas PS, Sharma VK, Singh YP, Gupta Raj K. Performance of 31 tree species and soil conditions in a plantation established with saline irrigation [J]. For Ecol Manage, 2003, 177 (1): 333-346 3 叶功富, 张立华, 林益明, 王亨, 周海超, 曾琦. 福建东山短枝木麻黄小枝氮磷含量及其再吸收率季节动态[J]. 生态学报, 2009, 29 (12): 6519-6526 [Ye GF, Zhang LH, Lin YM, ZhouHC, Zeng Q. Seasonal dynamics of nitrogen and phosphorus concentrations and nutrient resorption efficiencies of Casuarina equisetifolia branchlets in Dongshan County, Fujian [J]. Acta Ecol Sin, 2009, 29 (12): 6519-6526] 4 叶功富. 滨海沙地湿地松与木麻黄混交林构建和调控技术研究[J]. 林业科学, 2002, 15 (4): 463-468 [Ye GF. Studies on the Establishment and control techniques of mixed forest of Casuarina equisetifolia and Pinus elliottiiin coast zone [J]. Sci Silv Sin, 2002, 15 (4): 463-468] 5 林武星, 叶功富, 谭芳林, 聂森, 徐俊森. 沙岸木麻黄防护林不同更新模式土壤结构分形特征及其效应[J]. 中国生态农业学报, 2008, 16 (6): 1352- 1357 [Lin WX, Ye GF, Tan FL, Nie S, Xu JS. Fractal feature of soil structure and reflection on soil properties in different Regeneration patterns of Casuarina equisetifolia forest in coastal sandy soil [J]. Chin J Eco-Agric, 2008, 16 (6): 1352-1357] 6 林武星, 洪伟, 叶功富. 木麻黄根系浸提液对幼苗营养吸收和生长的影响[J]. 浙江林学院学报, 2005, 22 (2): 170-175 [Lin WX, Hong W, Ye GF. Effect of water extract of Casuarina equisetifoliaroot on nutrient absorption and growth of the seedlings [J]. J Zhejiang For Coll, 2005, 22 (2): 170-175] 7 林武星, 洪伟, 叶功富. 木麻黄水浸提液对其幼苗生长的影响[J]. 江西农业大学学报, 2005, 27 (1): 46-51 [Lin WX, Hong W, Ye GF. Effects of water extract from Casuarina equisetifolia on its seedling growth [J]. Acta Agric Univ Jiangxiensis, 2005, 27 (1): 46-51] 8 李键, 谢海慧, 刘奕, 洪滔, 林勇明, 林晗, 陈灿, 吴承祯, 洪伟. 两种木麻黄化感物质对其水培幼苗根系活力及内源激素含量的动态影响[J]. 热带作物学报, 2012, 33 (11): 2091-2097 [Li J, Xie HH, Liu Y, Hong T, Lin YM, Lin H, Cheng C, Wu CZ, Hong W. Effect of two allelochemicals of Casuarina equisetifoliaon dynamic change of root activity and endogenous hormone of its seedling under hydroponic cultivation [J]. Chin J Trop Crops, 2012, 33 (11): 2091-2097] 9 李键, 刘奕, 洪滔, 林勇明, 吴承祯, 洪伟. 2种化感成分对木麻黄幼苗小枝活性氧含量和保护酶活性的影响[J]. 植物资源与环境学报, 2013, 22 (2): 30-38 [Li J, Liu Y, Hong T, Lin YM, Wu CZ, Hong W. Effect of two allelochemicals on reactive oxygen content and protective enzyme activity in branchlet of Casuarina equisetifoliaseedling [J]. J Plant Resour Environ, 2013, 22 (2): 30-38] 10 李键, 刘奕, 吴承祯, 洪滔, 林勇明, 林晗, 陈灿, 洪伟. 木麻黄的两种化感物质对其水培幼苗叶绿素荧光参数的影响[J]. 应用与环境生物学报, 2013, 19 (5): 781-786 [Li J, Liu Y, Wu CZ, Hong T, Lin YM, Lin H, Cheng C, Hong W. Effect of two allelochemicals of Casuarina equisetifolia L. on chlorophyll fluorescence parameters in hydroponically cultured seedling [J]. Chin J Appl Environ Biol, 2013, 19 (5): 781-786] 11 Chen Y, Ren CG, Yang B, Peng Y, Dai CC. Priming effects of the endophytic fungus Phomopsis liquidambari on soil mineral N transformations [J]. Microb Ecol, 2013, 65 (1): 16-170 12 Wang HW, Zhu H, Liang XF, Du W, Dai CC. Molecular cloning and expression of a novel laccase showing thermo- and acid-stability from the endophytic fungus Phomopsis liquidambari and its potential for growth promotion of plants [J]. Biotechnol Lett, 2014, 36 (1): 167-173 13 Hubbard M, Germida JJ, Vujanovic V. Fungal endophytes enhance wheat heat and drought tolerance in terms of grain yield and second-generation seed viability [J]. J Appl Microbiol, 2014, 116 (1): 109-122 14 Rice JS, Pinkerton BW, Stinger WC, Undersander DJ. Seed production in tall fescue as affected by fungal endophytes [J]. Crop Sci, 1990, 30 (6): 1303-1305 15 Breen JP. Acremonium endophyte interactions with enhanced plant resistance to insects [J]. Annu Rev Entomol, 1994, 39: 401-423 16 Ball DM. Significance of Endophyte Toxicosis and Current Practices in Dealing with the Problem in the United States. Neotyphodium/Grass Interactions [M]. New York: Plenum Press, 1997: 395-410 17 Peters S, Dammeyer B, Schulz B. Endophyte-host interactions I: plants defence reactions to endophytic and pathogenic fungi [J]. Symbiosis, 1998, 25 (1): 193-211 18 Cheplick GP. Recovery fromdrought stress in Lolium perenne (Poaceae): are fungal endophytes detrimental [J]. Am J Bot, 2004, 91(12): 1960-1968 19 Monnet F, Vaillant N, Hitmi A, Coudret A, Sallanon H. Endophytic Neotyphodiumloliiinduced tolerance to Zn stress in Lolium perenne [J]. Physiol Plant, 2001, 113 (4): 557-563 20 谢安强, 洪伟, 吴承祯, 文笑, 洪滔, 陈灿. 10株桉树内生真菌对尾巨桉(E.urophylla × E.grandis)光合作用的影响[J]. 福建林学院学报, 2011, 31 (1): 31-37 [Xie AQ, Hong W, Wu CZ, Wen X, Hong T, Cheng C. Effects of 10 strains of endogenesis fungi on photosynthesis in Eucalypt urophylla × E. grandis [J]. J Fujian Coll For, 2011, 31 (1): 31-37] 21 谢安强, 洪伟, 吴承祯. 桉树内生菌对尾巨桉幼苗抗寒生理指标的影响[J]. 林业科学, 2012, 48 (6): 170-174 [Xie AQ, Hong W, Wu CZ. Cold resistance nutrient characters with ten kinds of fungus in Eucalyptusurophylla × E. grandis [J]. Sci Silv Sin, 2012, 48 (6): 170-174] 22 谢安强, 洪伟, 吴承祯, 李键, 林晗. 内生真菌对缓解尾巨桉幼苗铝毒害影响研究[J]. 江西农业大学学报(自然科学版), 2013, 35 (2): 339-345 [Xie AQ, Hong W, Wu CZ, Li J, Lin H. The study of poison laxation with ten kinds of fungus in E. urophylla × E grandis [J]. Acta Agric Univ Jiangxiensis, 2013, 35 (2): 339-345] 23 谢安强, 洪伟, 吴承祯. 内生真菌对尾巨桉幼苗磷元素吸收的影响[J]. 浙江农林大学学报, 2013, 30 (6): 863-870 [Xie AQ, Hong W, Wu CZ. Phosphorus absorption with ten fungi inoculants in Eucalyptus urophylla × Eucalyptus grandis [J]. J Zhejiang A & F University, 2013, 30 (6): 863-870] 24 Zhang Y, Zhong CL, Chen Y, Chen Z, Jiang QB. Improving drought tolerance of Casuarina equisetifolia seedlingsby arbuscular mycorrhizas under glasshouse conditions [J]. New For, 2010, 40 (3): 261-271 25 Zhong CL, Mansour S, Nambiar-veetil M, Bogusz D, Franche C.Casuarina glauca: a model tree for basic research in actinorhizal symbiosis [J]. J Biosci, 2013, 38 (4): 815-823 26 Diagne N, Diouf D, Svistoonoff S, Kane A, Noba K, Franche C, Bogusz D, Duponnois R.Casuarina in Africa: Distribution, role and importance of arbuscular mycorrhizal, ectomycorrhizal fungi and Frankia on plant development [J]. J Environ Manage, 2013, 128: 204-209 27 邓桂兰, 孔垂华, 骆世明. 木麻黄小枝提取物的分离鉴定及其对幼苗的化感作用[J]. 应用生态学报, 1996, 7 (2): 145-149 [Deng GL, Kong CH, Luo SM. Isolation and identification of extract from Casuarnia equisetifolia branchlet and its allelopathy on seedling growth [J]. Chin J Appl Ecol, 1996, 7 (2): 145-149] 28 林燕青. 内生真菌对木麻黄幼苗生长影响的研究[D]. 福州: 福建农林大学, 2012 [Lin YQ. Study on the effects of endophytic fungi on Casuarina equisetifolia seedling [D]. Fuzhou: Fujian Agriculture and Forestry University, 2012] 29 Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA. Fungal endophytes limit Pathogen damage in a tropical tree [J]. PANS, 2003, 100 (26): 15649-15654 30 Prochazkova D, Sairam RK, Srivastava GC, Singh DV. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves [J]. Plant Sci, 2001, 161 (4): 765-771 31 Ke DS, Wang AG, Sun GC, Dong LF. The effect of active oxygen on the activity of ACC synthase induced by exogenous IAA [J]. Acta Bot Sin, 2002, 44 (5): 551-556 32 赵世杰, 许长成, 邹琦, 孟庆伟. 植物组织中丙二醛测定方法的改进[J]. 植物生理学通讯, 1994, 30 (3): 207-210 [Zhao SJ, Xu CC, Zou Q, Meng QW. Improvements of method for measurement of malondialdehvde in plant tissues [J]. Plant Physiol J, 1994, 30 (3): 207-210] 33 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 164-169 [Li HS. Experimental Principle and Technology of Plant Physiology and Biochemistry [M]. Beijing: Higher Education Press, 2000: 164-169] 34 Lewis GC. Effects of biotic and abiotic stress on the growth of three genotypes of Lolium perenne with and without infection by the fungal endophyte Neotyphodium lolii [J] . Ann Appl Biol, 2004, 144 (1): 53-56 35 Hesse U, Schoberlein W, Wittenmayer L, Forster K, Warnstorff K, Diepenbrock W, Merbach W. Influence of water supply and endophyte infection (Neotyphodium spp.) on vegetative and reproductive growth of two Lolium perenne L. genotypes [J]. Eur J Agron, 2005, 22 (1): 45-54 36 任安芝, 高玉葆, 李侠. 内生真菌感染对黑麦草若干抗旱生理特征的影响[J]. 应用与环境生物学报, 2002, 8 (5): 535-539 [Ren AZ, Gao YB, Li X. Effect of fungal endophyte infection on some physiological characters of Lolium perenne under drought conditions [J]. Chin J Appl Rnviron Biol, 2002, 8 (5): 535-539] 37 Marks S, Clay K. Physiological responsesofFestucaarundinacea to fungal endophyte infection [J]. New Phytol, 1996, 133 (4): 727-733 38 袁志林, 章初龙, 林福呈. 植物与内生真菌互作的生理与分子机制研究进展[J]. 生态学报, 2008, 28 (9): 4430-4439 [Yuan ZL, Zhang CL, Lin FC. Recent advances on physiological and molecular basis of fungal endophyte-plant interactions [J]. Acta Ecol Sin, 2008, 28 (9): 4430-4439] 39 韩荣, 李夏, 任安芝, 高玉葆. 干旱胁迫下内生真菌感染对羽茅的生理生态影响[J]. 生态学报, 2011, 31 (8): 2115-2123 [Han R, Li X, Ren AZ, Gao YB. Physiological ecological effect of endophyte infection on Achnatherum sibiricum under drought stress [J]. Acta Ecol Sin, 2011, 31 (8): 2115-2123] 40 Malinowski DP, Belesky DP. Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance [J]. Crop Sci, 2000, 40 (4): 923-940


Last Update: 2016-06-25