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Effects of endophyte infection on reactive oxygen content and protective enzyme activity in branchlet of Casuarina equisetifolia seedling under stress of two allelochemicals*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 03
Page:
462-472
Research Field:
Articles
Publishing date:

Info

Title:
Effects of endophyte infection on reactive oxygen content and protective enzyme activity in branchlet of Casuarina equisetifolia seedling under stress of two allelochemicals*
Author(s):
LONG Feng1 HONG Tao1 LIN Yongming1 XIE Anqiang1 WU Chengzhen12 HONG Wei1 & LI Jian1**
1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2College of Ecology and Resource Engineering, Wuyi University, Nanping 354300, China
Keywords:
allelochemical stress endophyte Casuarina equisetifolia Forst. reactive oxygen content protective enzyme activity
CLC:
Q945.78 : Q948.122.3
PACS:
DOI:
10.3724/SP.J.1145.2015.11014
DocumentCode:

Abstract:
This research aimed to explore the possibility of using endophytic fungi-Casuarina equisetifolia Forst. seedling symbiont to alleviate the continuous?planting?obstacles. It was based on the previous research on the effect of two allelochemicals (quercetin-3-α-araboside, Q3A; quercetin-3-β-glucoside, Q3B) on the reactive oxygen contents and activities of scavenging enzymes in branchlets of Casuarina equisetifolia Forst. seedlings free (EF) from endophyte (Aspergillus sp.). We studied the effect of Q3A and Q3B on the reactive oxygen contents and activities of scavenging enzymes in branchlets of Casuarina equisetifolia Forst. Seedlings infected with (EI) endophyte (Aspergillus sp.) by addition of allelochemicals to the water (of seven concentrations: 0, 12.5, 25, 50, 100, 200 and 400 mg/L) for 0-72 h to analyse the effect of endophyte infection. The results showed that the endophytic fungus colonization alleviated the allelochemical stress intensity to some degree and the effect was obvious in slight allelochemical stress at the early stage (100 mg/L 0-12 h, 50 mg/L 0-24 h, 25 mg/L 0-36 h, 12.5 mg/L 0-48 h), mainly in the reactive oxygen and its scavenging system; endophyte infection significantly reduced the production of hydrogen peroxide (H2O2), malondlaldehyde (MDA) and superoxide radical (O2-). At the same time, endophyte infection significantly improved the activity of protective enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT). But the effective concentration and stress time was in accordance with those that Casuarina equisetifolia Forst. EF endured, which showed that the protective enzyme system of C. equisetifolia. EI which was improved by endophytic fungus needed to be built before protective enzyme system synthesis and degradation suffered from lethal harms. EI had higher activities of protective enzyme activity under Q3A stress than under Q3B stress. The results of our experiment indicated that endophyte infection is beneficial to host growth under Q3A stress.

References

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Last Update: 2016-06-25