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The effect of culture condition on synthesis of nanoparticles lead sulfide byDesulfovibrio desulfuricans*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 02
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The effect of culture condition on synthesis of nanoparticles lead sulfide byDesulfovibrio desulfuricans*
GONG Jun1** SONG Xiaomin2 GAO Yan2 BAI Hongjuan2 WANG Yufen1 & HAN Jinxian1
1Department of Environmental Economics, Shanxi University of Finance and Economics, Taiyuan 030006, China2College of Chemical & Environment Engineering, North University of China, Taiyuan 030051, China
Desulfovibrio desulfuricans nanoparticles PbS synthesis pH temperature

Nanoparticles PbS is synthesized by Desulfovibrio desulfuricans under different conditions with advantages ofsimplicity, stability and small particle size, which is a good example of biological synthesis of nanometer products. Thisresearch investigated the effects of processing variable changes including pH and temperature on the synthesis of nanoparticlesPbS products by D. desulfuricans. The microstructure of the PbS nanoparticles was analyzed by XRD, EDS and HRTEM,respectively. And the mechanism was also studied. The conversion rate of Pb2+ as over 98% under all pH and temperatureconditions, up to 99.7% with pH 7 and 30 °C. With the increase of pH from 5 to 8, the shape of PbS nanoparticles changed fromspheroidal to rod and the particle sizes enlarged from 10-15 nm to 15-30 nm. The monodisperse irregular nanoparticles PbSwere produced with 10-25 nm under pH 9. The temperature change between 10 °C and 35 °C had no effect on the shape or sizeof nanoparticles PbS, which kept irregular rods and 15-30 nm. Nanoparticles PbS was produced by Pb2+ and sulfide in the cellsand released into the solution. The results indicated that the pH change may cause morphology variation in PbS nanoparticles,but the temperature has little impact on them. D. desulfuricans has high conversion rate of Pb2+. Nanoparticles PbS can beformed in the cells.


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