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The trapping of rhizobia in waste V-Ti magnetite mine soils and theirsymbiotic nitrogen-fixing effects*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 02
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The trapping of rhizobia in waste V-Ti magnetite mine soils and theirsymbiotic nitrogen-fixing effects*
KANG Xia1 ZHENG Wenwen1 QU Kui1 CUI Yongliang2 LI Yanmei1 CHEN Qiang1 & YU Xiumei1**
1Faculty of Resources, Sichuan Agricultural University, Chengdu 611130, China2Sichuan Provincial Academy of Natural Resources, Chengdu 610041, China
rhizobia molecular identification waste V-Ti magnetite soil symbiotic nitrogou fixation sio-remediation

The symbiosis of rhizobia and leguminous plants can provide nitrogen, enhance tolerance capacity for plants andincrease nitrogen accumulation in soil. It has been utilized in the bio-remediation of metalliferous sites. For the purpose ofbio-remediate the waste V-Ti magnetite mine soil, this research aimed to select native rhizobia with strong tolerance capacityagainst heavy metals. Using pot-wllture experiment method, soybean and cowpea were planted in the soil of a waste V-Timagnetite mine at Panzhihua, Sichuan Province, to capture and isolate the endophytic bacteria from root nodules. Rhizobiawere selected by molecule identification of BOX-A1R PCR fingerprinting and phylogenetic analysis of 16s rDNA. Plantexperiments using soybean and cowpea were performed in both waste mine soil and vermiculite with inoculation to assess thesymbiosis effect of rhizobia with legumes. As a result, a total of 43 endophytic bacteria were obtained from the root nodulesof soybean and cowpea. These 43 strains were clustered into 13 groups with a similarity level of 84%. Fifteen representativestrains were selected from the 13 groups to sequence for their 16S rDNA and construct phylogenetic tree, which showed thatonly ms12-11 and syj1 from group 8 were rhizobia. Both were identified as belonging to the genus of Bradyrhizobium. In thepot culture experiment with inoculations, both Bradyrhizobium sp. ms12-11 and syj1 could form effective nitrogen-fixingassociations with soybean and cowpea respectively in barren waste mine soil with high content of heavy metals. Nevertheless,the nitrogen-fixing efficiency of ms12-11 was significant higher than that of syj1. Due to the stress of heavy metals, thenitrogen-fixing efficiency of soybean and cowpea planted in the waste mine soil was significant lower than that in vermiculite.This study indicated that there are abundant rhizobia in the waste V-Ti magnetite mine soil. They can establish nitrogen-fixingsymbiosis with leguminous plants in adverse environment to strengthen plants’ tolerance against stress.


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