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Laccase-mediator system: a review(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 06
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Laccase-mediator system: a review
LUO Shuang XIE Tian LIU Zhongchuan WANG Ganggang
1Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2Key Laboratory of Environmental Microbiology of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 3University of Chinese Academy of Sciences, Beijing 100049, China
laccase mediator oxidation mechanism LMS laccase stability industrial application
Q554 : X17

Laccase is a polyphenol oxidase being able to oxidize phenolic and non-phenolic substrates. Though hindered by its low redox potential and substrate accessibility, laccase as an environment-friendly enzyme, has been employed in various industrial processes including lignin degradation, biodegradation or transformation of environmental pollutants. Discovery of the mediator for laccase greatly relieved such hindrance of its application. The laccase-mediator system (LMS) strengthens the capability of laccase and enlarges its substrate accessibility. In this paper, the characteristic and classification of mediator, the mechanisms of LMS, industrial applications and laccase stability were reviewed. Currently, the mediators are classified into three categories, namely the synthetic mediator, the natural mediator and the others (e.g. polyoxometalates, POM). Compared to the other two mediators, the natural mediators are cost-effective, less toxic, more efficient and easily available. Three mechanisms have been proposed for the function of mediators in LMS: 1) hydrogen atom transfer (HAT); 2) electron transfer (ET); and 3) ionic mechanism type (IM). The laccase-mediator system has been found useful for diverse biotechnological applications. Laccase-mediator system functions efficiently not only in bleaching wood pulp and non-wood pulp but also in improving pulp properties. In addition, the LMS performs excellently in removing stubborn dyes and degrading refractory pollutants and hydroxy polychlorinated biphenyls contamination. In addition, the addition of natural mediator could increase the stability of laccase in the LMS. However, the high ratio of the mediator to substrate and the regeneration of the mediator in the LMS are still problems to be solved, which demand extensive investigation in further studies.


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