|Table of Contents|

Medium optimization for β-glucosidase production from Penicillium sp. and preparation of soluble inducer*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 03
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Medium optimization for β-glucosidase production from Penicillium sp. and preparation of soluble inducer*
LI Yonghao1 ZHANG Xiaoyue1 ZHAO Xinqing2** & BAI Fengwu12
1School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China 2School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
β-glucosidase Penicillium response surface optimization transglycosylation reaction soluble inducer sophorose cellulase
TQ353 : TQ925

Production of biofuels and bio-based chemicals from lignocellulosic materials have received worldwide attention. However, high cost cellulase production is one of the major bottlenecks for commercial production. Compared with insoluble cellulose materials, employment of soluble inducer could avoid the high mass transfer barrier in the bioreactor and difficulty in feeding. Therefore, development of efficient and low cost inducer is of great importance for low cost cellulase production. Low cost soluble inducer can be prepared by transglycosylation reaction using β-glucosidase. To achieve low cost and efficient β-glucosidase production, Penicillium sp. YH02 isolated by our lab was used as a producer strain, and wheat bran, barley grass and microcrystalline cellulose in the fermentation medium were further optimized for β-glucosidase production by response surface methodology. The β-glucosidase production was increased by 15.03% after optimization. Moreover, glucose-disaccharide mixture (GDM) was prepared using β-glucosidase produced by Penicillium sp. YH02, which was used as carbon source and inducer for cellulase production by Trichoderma reesei Rut C30. Filter Paper Activity (FPA) reached 1.12 IU/mL when GDM was supplied at the concentration of 10 g/L, which was 24.9 times higher compared with that using glucose. Sophorose, gentiobiose and cellobiose were detected in GDM analyzed by ion chromatography, among which sophorose is known as the most efficient soluble inducer for cellulase production of T. reesei. The results in this study benefit further development of low cost inducer for reducing the production cost of cellulase.


1 Dionisi D, Anderson JA, Aulenta F, McCue A, Paton G. The potential of microbial processes for lignocellulosic biomass conversion to ethanol: a review [J]. J Chem Technol Biot, 2015, 90: 366-383 2 Sánchez C. Lignocellulosic residues: biodegradation and bioconversion by fungi [J]. Biotechnol Adv, 2009, 27: 185-194 3 Cherry JR, Fidantsef AL. Directed evolution of industrial enzymes: an update [J]. Curr Opin Biotech, 2003, 14: 438-443 4 谢天文, 刘晓风, 袁月祥, 闫志英, 贺蓉娜, 廖银章. 真菌产纤维素酶的诱导物及其调控机理研究进展[J]. 应用与环境生物学报, 2010, 16 (3): 440-444 [Xie TW, Liu XF, Yuan YX, Yan ZY, He RN, Liao YZ. Progress in research of inducers and regulation mechanism of fungal cellulase [J]. Chin J Appl Environ Biol, 2010, 16 (3): 440-444] 5 Aro N, Pakula T, Penttil? M. Transcriptional regulation of plant cell wall degradation by filamentous fungi [J]. FEMS Microbiol Rev, 2005, 29: 719-739 6 Mandels M, Parrish FW, Reese ET. Sophorose as an inducer of cellulase in Trichoderma viride [J]. J Bacteriol, 1962, 83: 400-408 7 Nogawa M, Goto M, Okada H, Morikawa YL, Sorbose induces cellulase gene transcription in the cellulolytic fungus Trichoderma reesei [J]. Curr Genet, 2001, 38: 329-334 8 Kou Y, Xu J, Cao Y, Lv X, Zhao G, Chen G, Zhang W, Liu W. Gluconolactone induces cellulase gene expression in cellulolytic filamentous fungus Trichoderma reesei [J]. RSC Adv, 2014, 4: 36057-36063 9 Chen S, Wayman M. Novel inducers derived from starch for cellulase production by Trichoderma reesei [J]. Process Biochem, 1992, 27: 327-334 10 Lo CM, Zhang Q, Callow NV, Ju LK. Cellulase production by continuous culture of Trichoderma reesei Rut C30 using acid hydrolysate prepared to retain more oligosaccharides for induction [J]. Bioresource Technol, 2010, 101: 717-723 11 Ajisaka K, Nishida H, Fujimoto H. The synthesis of oligosaccharides by the reversed hydrolysis reaction of β-glucosidase at high substrate concentration and at high temperature [J]. Biotechnol Lett, 1987, 9: 243-248 12 黄振艳, 夏黎明. 利用葡萄糖转苷酶制备纤维素酶可溶性诱导物的研究[J]. 高校化学工程学报, 2009, 23, 270-274 [Huang ZY, Xia LM. Study on the soluble inducer for cellulase production derived from glucose by glucosylase [J]. J Chem Eng Chinese U, 2009, 23: 270-274] 13 张晓月, 孜力汗, 李勇昊, 赵心清, 白凤武. 里氏木霉Rut-C30产纤维素酶培养基优化及其酶解特性[J]. 过程工程学报, 2014, 14 (2): 312-318 [Zhang XY, Zi LH, Li YH, Zhao XQ, Bai FW. Optimization of the fermentation medium for cellulase production from Trichoderma reesei Rut-C30 and its enzymatic hydrolysis characteristics [J]. Chin J Process Eng, 2014, 14 (2): 312-318] 14 Rastall R, Pikett S, Adlard M, Bucke C. Synthesis of oligosaccharides by reversal of a fungal β-glucanase [J]. Biotechnol Lett, 1992, 14: 373-378 15 Ghose T. Measurement of cellulase activities [J]. Pure Appl Chem, 1987, 59: 257-268 16 Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar [J]. Anal Chem, 1959, 31, 426-428 17 黄玉红, 靳艳玲, 方扬, 赵海. 细胞壁多糖水解酶及其在非粮生物质原料转化中的应用与研究进展[J]. 应用与环境生物学报, 2013, 19 (5): 881-890 [Huang YH, Jin YL, Fang Y, Zhao H. Application and progress of plant cell wall polysaccharide hydrolase in nonfood based biomass conversation [J]. Chin J Appl Environ Biol, 2013, 19 (5): 881-890] 18 Liu G, Qin Y, Li Z, Qu Y. Development of highly efficient, low-cost lignocellulolytic enzyme systems in the post-genomic era [J]. Biotechnol Adv, 2013, 31: 962-975 19 袁晓华. β-葡萄糖苷酶产生菌的筛选、培养条件优化及β-葡萄糖苷酶应用研究[D]. 济南: 山东大学, 2009 [Yuan XH. Screening, identified and culture condition optimization of β-glucosidase producing microorganism and β-glucosidase application [D]. Jinan: Shandong university, 2009] 20 Vaheri MP, Vaheri ME, Kauppinen VS. Formation and release of cellulolytic enzymes during growth of Trichoderma reesei on cellobiose and glycerol [J]. Eur J Appl Microbiol Biotechnol, 1979, 8: 73-80 21 Kurasawa T, Yachi M, Suto M, Kamagata Y, Takao S, Tomata F. Induction of cellulase by gentiobiose and its sulfur-containing analog in Penicillium purpurogenum [J]. Appl Environ Microb, 1992, 58: 106-110 22 孙群, 牟世芬, 陆德培. 单糖和寡糖的离子色谱法分析研究[J]. 化学通报, 1991 (8): 39-41 [Sun Q, Mu SF, Lu DP. Study on the monosaccharides and oligosaccharides derived from ion chromatography [J]. Chemistry, 1991 (8): 39-41]


Last Update: 2016-06-25