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Effect of overexpression of chorismate mutase encoding gene ARO7 on theinhibitor tolerance of Saccharomyces cerevisiae(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 02
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Effect of overexpression of chorismate mutase encoding gene ARO7 on theinhibitor tolerance of Saccharomyces cerevisiae
ZHANG Mingming1 WAN Qingqing2 ZHANG Keyu2 XIONG Liang1 BAI Fengwu1 2 & ZHAO Xinqing2**
1School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China2State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
cellulosic ethanol Jerusalem artichoke stalks Saccharomyces cerevisiae aromatic amino acid ARO7 acetic acidyeast stress tolerance

Fuel ethanol production using lignocellulosic materials has attracted widespread attention. However, inhibitorycompounds (e.g. weak acid, phenol and furfural) released from the pretreatment process exert inhibition on yeast cell growthand ethanol fermentation. Therefore, improvement of inhibitors tolerance of Saccharomyces cerevisiae benefits efficientcellulosic ethanol production. In this work, chorismate mutase encoding gene ARO7 which participates in aromatic aminoacid biosynthesis was overexpressed in S. cerevisiae, the cell growth and ethanol production of the recombinant strain in thepresence of inhibitory compounds were evaluated. It was found that the ARO7 overexpressing strain grew better than thecontrol strain in the presence of 5.0 g/L acetic acid. Similar result was found when ethanol fermentation was performed with 5.0g/L acetic acid addition. In addition, ethanol yield of the ARO7 overexpressing strain was improved from 0.44 g/g to 0.47 g/gglucose, and the ethanol productivity increased 22.38% when compared with the control strain using Jerusalem artichoke stalkshydrolysate. This work demonstrated that overexpression of ARO7 would be a feasible strategy to increase cellulosic ethanolproduction efficiency in the presence of inhibitors.


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Last Update: 2016-04-25