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Optimization of fermentation conditions for lipid production by fungus withsweet potato starch hydrolysates*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2016 02
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Optimization of fermentation conditions for lipid production by fungus withsweet potato starch hydrolysates*
FENG Shunli XU Hui LI Xinran ZHOU Huoxiang QIAO Dairong & CAO Yi**
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China
Gibberella intermedia sweet potato starch microbial oil fermentation condition optimization
Q939.9 : TK6

Microbial oil is a potential raw material for biodiesel. In order to reduce the cost of microbial lipid production, it isnecessary to screen high oil producing strain that can make full use of sweet potato starch sugar and optimize the fermentationconditions. In the present study, the strain named Gibberella intermedia (S-1) identified by 5.8S rDNA-ITS sequence wasscreened by using sweet potato starch sugar as carbon source. To optimize the medium composition and fermentationconditions for lipid production by the strain S-1, experiments with single factor and orthogonal test were conducted. The strainS-1 had an initial biomass of 15.82 g/L, lipid content of 31.51% and lipid yield of 4.98 g/L, which increased to 21.67 g/L,39.87% and 8.64 g/L, respectively, after optimization by single factor and orthogonal test. The lipid composition of the strainS-1 was determined by GC-MS. The results showed that the oil similar to vegetable oil, with 89.82% of it unsaturated fattyacid; the main contents were palmitic acid, oleic acid and linoleic acid, suitable to produce biodiesel. This research thereforesuggested that the strain S-1 is able to take full advantage of the relatively cheap raw materials for microbial oil production andis a potential strain for biodiesel production.


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Last Update: 2016-04-25