|Table of Contents|

The effect of hierarchical matrix culturing on the growing efficiency ofOxytropis ochrocephala Bunge seedlings*(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 02
Page:
236-240
Research Field:
Articles
Publishing date:

Info

Title:
The effect of hierarchical matrix culturing on the growing efficiency ofOxytropis ochrocephala Bunge seedlings*
Author(s):
ZHANG Ming1 YANG Jiajun1 ZHANG Xiuhong2 GUO Bin1 FU Yanping1 & HE Wei1**
1Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory ofBiotechnology, College of Life Sciences, Center for Poisonous Plants Research in Western China, Northwest University, Xi’a
Keywords:
Oxytropis ochrocephala Bunge seedlings hormone treatment hierarchical matrix growth efficiency
CLC:
Q949.751.905
PACS:
DOI:
10.3724/SP.J.1145.2015.07016
DocumentCode:

Abstract:
Oxytropis ochrocephala Bunge is a toxic perennial grass on the grassland of western China that causes great threatto the animals and the stability of grassland ecology. O. ochrocephala grows slowly with high death rate under laboratoryconditions. To provide research basis for future studies on controlling O. ochrocephala, this research aimed to determine itsoptimal laboratory growing conditions. We treated the growth matrix hierarchically and applied hormones including GA3,IAA, and NAA for germinated seeds of O. ochrocephala to search for the best cultivating conditions for the improvement ofgrowth cycle and the seedlings survival rate. The results showed an increase of 98% in germination rate with watering of 5 mg/L GA3, IAA 3 mg/L and 3 mg/L NAA. Then we planted the seedlings in the hierarchical matrix composed of a 30% sand layerand 70% humus soil layer from top to bottom to induce the formation of roots and watered the plant with 5 mg/L IBA. Statisticsshowed that the average survival rate was improved from 10% to 67%, and the average plant height increased from 0.70 (± 0.070)cm to 2.77 (± 0.711) cm. The results showed that the optimal treatment (5 mg/L GA3, IAA 3 mg/L and 3 mg/L NAA, 30% sandlayer and 70% humus soil layer) effectively increased growth efficiency and survival rate of O. ochrocephala seedling.

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Last Update: 2016-04-25