|Table of Contents|

Species-specific evolution of glycerol-3-phosphate dehydrogenase (GPDH) gene family in plant(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2016 01
Page:
75-84
Research Field:
Articles
Publishing date:

Info

Title:
Species-specific evolution of glycerol-3-phosphate dehydrogenase (GPDH) gene family in plant
Author(s):
LANG Xiaoqiang WEI Congchong QIN Shishang CAO Yu XU Hui QIAO Dairong & CAO Yi**
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China
Keywords:
glycerol-3-phosphate dehydrogenase phylogenetic analysis functional divergence analyses selection pressure adaptive evolution analyses
CLC:
Q943.2
PACS:
DOI:
10.3724/SP.J.1145.2015.05018
DocumentCode:

Abstract:
Glycerol-3-phosphate dehydrogenase (GPDH) is a kind of enzyme that plays an important role in phosphoric acid glyceride metabolism and regulates the process of glyceride metabolism by catalyzing the reversible redox conversion of dihydroxyacetone phosphate to SN-glycerol 3-phosphate. To better understand the evolutionary history and diversity of the GPDH gene family in plants, a series of genome-wide analysis was executed in our work. We acquired 20 known GPDH sequences through previous researches and the Genbank and other relevant databases. Then we used these sequences as query sequences to perform multiple Blast searches against plants whole-genome to retrieve GPDH-like sequences and identify the new members of the GPDH protein family in plants. Then we constructed the phylogenetic tree and performed gene structure and protein motif analyses, functional divergence, Ka/Ks ratio and adaptive evolution analyses. As a result, a total of 75 GPDH members were identified to belong to 2 clades and 3 monophyletic lineages, indicating that the GPDH genes arose from two ancestral GPDH genes. Functional divergence analyses showed that significant amino acid site-specific selective constraints acted on the different clades of GPDH genes. Adaptive evolution analyses and Ka/Ks ratioanalyses demonstrated that GPDH genes were subject to strong purifying selection after their species-specific duplications and that rapid evolution occurred with a high degree of evolutionary dynamics in GPDH genes. In conclusion, this study provides a better understanding of the evolutionary history and diversity of the GPDH gene family in plants, and offeres a foundation for further biochemical and genome-wide analyses and genetic studies of the GPDH gene family in plants. Moreover, the findings in this work provide insight into the function and evolution of this gene family in plant and pave the way for studies in other species.

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Last Update: 2016-02-25