|Table of Contents|

Seasonal variation of bacterial community in sediments of urban eutrophic lakes(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2015 06
Page:
1012-1018
Research Field:
WRHJ
Publishing date:

Info

Title:
Seasonal variation of bacterial community in sediments of urban eutrophic lakes
Author(s):
PENG Lei ZHAO Jianwei ZHANG Yu HUA Yumei ZHU Duanwei LIU Guanglong
1Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Wuhan 430070, China 2Laboratory of Ecological and Environmental Engineering of Huazhong Agricultural University, Wuhan 430070, China
Keywords:
lake sediment seasonal variation bacterial community PCR-DGGE Proteobacteria
CLC:
Q938.8
PACS:
DOI:
10.3724/SP.J.1145.2015.05011
DocumentCode:

Abstract:
Bacteria are very sensitive to environmental changes in the sediment. Little is known about the seasonal variations of bacterial community in sediments of the urban eutrophic lakes. This study aimed to reveal the seasonal variations of the abundance and structure of bacterial community in sediments of Donghu Lake and Nanhu Lake in Wuhan, China. PCR- DGGE and cloning sequencing were used to measure the diversity and seasonal variations of bacterial communities in sediments of two eutrophic lakes. The results showed that urban lake sediments were abundant in bio-diversity of bacterial community with a total of 287 DGGE bands in winter and 317 DGGE bands in summer. For Donghu Lake, the average species richness values were 27.4 in winter and 31.0 in summer, respectively, and the Shannon-Wiener diversity indices were 2.32 in winter and 2.83 in summer. For Nanhu Lake, the average species richness values were 30.0 in winter and 32.2 in summer, and the Shannon-Wiener diversity indices were 2.83 in winter and 2.86 in summer. It was obviously that the Shannon-Wiener diversity indices and species richness values were higher in summer than in winter in both lakes. The reason might be that the higher temperature in summer could promote the growth of microorganisms in lakes and improve bio-diversity of bacterial community. The sediment samples in the same lake showed high similarity, suggesting that each lake formed its own unique internal structure of bacterial communities. The results of cloning sequencing showed that the main bacterial phyla in Donghu Lake and Nanhu Lake were Proteobacteria, Bacteroidetes and Firmicutes, with Proteobacteria being dominant, accounting for 77% of the total number of sequences, followed by Bacteroidetes (17%) and Firmicutes (6%). In the Proteobacteria phylum, Alphaproteobacteria and Betaproteobacteria were predominant, accounting for 35% and 31%, respectively, of the total number of sequences. Our results suggested that the abundance and structure of bacterial community in sediments of the urban eutrophic lakes would vary with the season. This conclusion enriches the understanding of bacterial community in sediments of urban eutrophic lakes.

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Last Update: 2016-01-04