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Genotoxicity evaluation based on luminescent bacteria Acinetobacter sp. RecA for seawater contaminated by Huangdao oil spill(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 04
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Genotoxicity evaluation based on luminescent bacteria Acinetobacter sp. RecA for seawater contaminated by Huangdao oil spill
LUAN Xiao CUI Zhisong GAO Wei WEN Xiaolong XU Guangfei LI Qian SUN Chengjun ZHENG Li1
1Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 2Institute of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Huangdao oil spill petroleum hydrocarbons luminescent bacteria genotoxicity
X824 : X55

After the Sinopec pipeline explosion on Nov. 22, 2013 at Huangdao, China, local beaches and sea water at Jiaozhou Bay was polluted by crude oil to different degrees. This study investigated the ecological effect of petroleum hydrocarbons in seawater on marine organisms with a rapid genotoxicity detection method based on the luminescent bacteria Acinetobacter sp. RecA. We collected seawater samples from five sites 1 km to 15 km away from the oil leakage point during a time course of 90 days. The chemical composition of petroleum hydrocarbons in the seawater samples were analyzed by GC-MS. Meanwhile, the genotoxicity of each sample was determined by Acinetobacter sp. RecA using mitomycin C (MMC) as reference standard toxicant. The results showed that the petroleum hydrocarbon contents of 3 samples from sites S1, S2, and S3 within 1 km from the oil leakage point were from 0.55 mg/L to 1.04 mg/L. The corresponding genotoxicity was equivalent to 0.60 mg/L to 1.78 mg/L of MMC. The genotoxicity level decreased as the petroleum hydrocarbon content gradually decreased to < 0.02 mg/L over time. The samples from sites S4 and S5 which were over 5 km away from the oil leakage point had low petroleum hydrocarbons content (< 0.02 mg/L) and exhibited low or no genotoxicity during the 90 days. The results suggested that as a whole cell biosensor, luminescent bacteria Acinetobacter sp. RecA can quickly and accurately detect genotoxicity of contaminated sea water. This method has great potential in providing technical support after marine oil spill. It can also be used to monitor the dynamics for environmental risk assessment of marine pollution.


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Last Update: 2015-08-24