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Extraction conditions and in vitro antioxidant activities of polyphenols from Dendrobium nobile(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 04
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Extraction conditions and in vitro antioxidant activities of polyphenols from Dendrobium nobile
FEI Wen CHUN Ze HE Qinyi WANG Miaoyu LUO Aoxue FAN Yijun
1Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China 2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
Dendrobium nobile polyphenols response surface methodology antioxidant

Dendrobium nobile has various health care functions and therefore of prominent medicinal value. This study aimed to find out optimal extraction conditions of polyphenols from Dendrobium nobile and to understand their antioxidant activity in vitro. With a single-factor experiment, the main parameters affecting the yield of phenolics were defined, including the pH of solvent, ethanol concentration and ratio of water to raw material. The antioxidant activities of the total polyphenols extract were evaluated by ABTS radicals, DPPH radicals and hydroxyl radicals using Vc as control. The optimal extraction parameters for total polyphenols yield were ethanol concentration 72% (V/V), ratio of water to raw material as 1:50 (g/mL), pH of 3, to obtain an extraction yield of total phenolic of 0.862. With the concentration of total phenolic from Dendrobium nobile as 5 mg/mL, the radical scavenging activity in clearing ABTS, DPPH and OH free radicals was 100%, 78.54% and 49.1%, respectively. The results suggested that response surface methodology is applicable for optimizing extraction conditions of total polyphenols from Dendrobium nobile, and that the total polyphenols from Dendrobium nobile have good scavenging activity in a close-dependent manner.


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Last Update: 2015-08-24