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Effect of acute copper stress on antioxidant and metal detoxification ability of the tadpole of crab eating frog (Fejervarya cancrivora)(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 03
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Effect of acute copper stress on antioxidant and metal detoxification ability of the tadpole of crab eating frog (Fejervarya cancrivora)
HONG Meiling LI Chunyu WANG Lijun TIAN Dandan LIU Weiwei
College of Life Sciences, Hainan Normal University, Haikou 571158, China
tadpole of crab eating frog copper stress antioxidants ability acetylcholine esterasemetallothionein mangrove forest

The crab eating frog (Fejervarya cancrivora) is the only amphibian in the mangrove forest. To explore the feasibility of using its tadpole as an indicator of the habitat quality of mangrove forests, we studied the effect of acute copper exposure on the activities of superoxide dismutase (SOD) and acetylcholine esterase (AChE) and the content of malonaldehyde (MDA) and metallothionein (MT) in the tadpole (Stage 38-39). The LC50 of Cu2+ was 5.69 mg L-1 for 24 h exposure, 3.37 mg L-1 for 48 h, 1.34 mg L-1 for 72 h, and 0.95 mg L-1 for 96 h exposure. Based on the 96h LC50, four treatments of Cu2+ (control, 80, 160, 300 μg L-1 CuSO4) were designed and sampled at 24 h, 48 h, 72 h and 96 h. The results showed that SOD and AChE activities and MT content in three treatment groups were lower than the control groups, and decreased significantly with the time and the increase of Cu2+ concentration. However, the trend was conversed in the content of MDA. Higher exposure concentration and longer exposure time led to higher MDA content, suggesting that the lipid peroxidation increased under copper stress. Also, there was an interaction effect of exposure time and Cu2+ content on all indicators except AChE. Therefore, the biomarkers of SOD and AChE activity and MDA content are sensitive to heavy metal induced oxidative stress, and Fejervarya cancrivora can be the indicator species for environment monitoring of mangrove forests.


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