|Table of Contents|

Advances in research of pollution, degradation and removal of antibiotics and antibiotic resistance genes in the environment(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 02
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Advances in research of pollution, degradation and removal of antibiotics and antibiotic resistance genes in the environment
SHEN Yiwen HUANG Zhiting XIE Bing
Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China
antibiotics antibiotic resistance genes horizontal gene transfer microbial community structure degradation mechanism removal efficiency
X172: X506

With the widespread application of antibiotics and other antimicrobic agents, the residuals and potential hazards in both natural and human environment have raised public concerns. Based on current research, our review documents the sources of antibiotic and antibiotic resistant genes (ARGs) and the contamination status, analyzes their relationships and propagation mechanisms, and summarizes treatment technologies. Antibiotics and ARGs are commonly detected in water, soil and air, with comparatively higher levels in human environment, including wastewater treatment plant and solid waste processing chains. ARGs are induced by antibiotic residuals accumulated in the environment, but ARGs propagation is dominated by horizontal gene transfer (HGT) and the composition of microbial communities. In addition, other factors including temperature, light, and microbial communities all could impose effects on the degradation of ARGs and antibiotics, among which light is the most crucial factor in natural environment. In the engineered treatment systems, UV disinfection and biochemical degradation function well in ARGs and antibiotic removal, though not always so. Given these results, the review suggests that future study should focus on the mechanisms of antibiotics and ARGs propagation as well as specific treatment technologies.


1 王丹, 隋倩, 赵文涛, 吕树光, 邱兆富, 余刚. 中国地表水环境中药物和个人护理品的研究进展[J]. 科学通报, 2014, 59 (9): 743-751 [Wang D, Sui Q, Zhao WT, Lv HG, Qiu ZF, Yu G. Pharmaceutical and personal care products in the surface water of China [J]. Chin Sci Bull, 2014, 59 (9): 743-751]
2 周启星, 罗义, 王美娥. 抗生素的环境残留、生态毒性及抗性基因污染[J]. 生态毒理学报, 2007, 2 (3): 243-249 [Zhou QX, Luo Y, Wang ME. Environmental residues and ecotoxicity of antibiotics and their resistance gene pollution: a review [J]. Asian J Ecotoxicol, 2007, 2 (3): 243-249]
3 王路光, 朱晓磊, 王靖飞, 田在锋. 环境水体中的残留抗生素及其潜在风险[J]. 工业水处理, 2009, 29 (5): 11-14 [Wang LG, Zhu XL, Wang JF, Tian ZF. Antibiotic residual in environmental water body and its potential risks [J]. Ind Water Treat, 2009, 29 (5): 11-14]
4 杨凤霞, 毛大庆, 罗义, 汪庆, 母全华. 环境中抗生素抗性基因的水平传播扩散[J]. 应用生态学报, 2013, 24 (10): 2993-3002 [Yang FX, Mao DQ, Luo Y, Wang Q, Mu QH. Horizontal transfer of antibiotic resistance genes in the environment [J]. Chin J Appl Ecol, 2013, 24 (10): 2993-3002]
5 Bayen S, Yi XZ, Segovia E, Zhou Z, Kelly BC. Analysis of selected antibiotics in surface freshwater and seawater using direct injection in liquid chromatography electrospray ionization tandem mass spectrometry [J]. J Chromatogr A, 2014, 1338 (18): 38-43
6 Zhang DD, Lin LF, Luo ZX, Yan CZ, Zhang X. Occurrence of selected antibiotics in Jiulongjiang River in various seasons, South China [J]. J Environ Monit, 2011, 13: 1953-1960
7 Sarmah AK, Meyer MT, Boxall ABA. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment [J]. Chemosphere, 2006, 65 (5): 725-759
8 Hu XG, Zhou QX, Luo Y. Occurrence and sourceanalysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetablebases, northern China [J]. Environ Pollut, 2010, 158 (9): 2992-2998
9 Guerra P, Kim M, Shah A, Alaee M, Smyth SA. Occurrence and fate of antibiotic, analgesic/anti-inflammatory, and antifungal compounds in five wastewater treatment processes [J]. Sci Total Environ, 2014, 473: 235-243
10 王朋华, 袁涛. 污水厂头孢类抗生素去除规律及潜在风险评估[J]. 上海交通大学学报, 2010, 44 (11): 1550-1555 [Wang PH, Yuan T. Removal of cephalosporin antibiotics in wastewater treatment plant and preliminary risk assessment [J]. J Shanghai Jiaotong Univ, 2010, 44 (11): 1550-1555]
11 García-Sánchez L, Garzón-Zú?iga MA, Buelna G, Moeller-Chávez GE, Noyola A, Avilez-Flores M, Estrada-Arriaga EB. Occurrence of tylosin in swine wastewater in Mexico [J]. Water Sci Technol, 2013, 68 (4): 894-900
12 梁惜梅, 施震, 黄小平. 珠江口典型水产养殖区抗生素的污染特征[J]. 生态环境学报, 2013, 22 (2): 304-310 [Liang XM, Shi Z, Huang XP. Occurrence of antibiotics in typical aquaculture of the Pearl River Estuary [J]. Ecol Environ Sci, 2013, 22 (2): 304-310]
13 张志强. 设施菜田土壤四环素类抗生素污染与有机肥安全施用[D]. 北京: 中国农业科学院, 2013 [Zhang ZQ. Study on soil tetracyclines antibiotics pollution and safe application of manure in greenhouse vegetable field [D]. Beijing: Chinese Academy of Agricultural Sciences, 2013]
14 冀秀玲, 沈群辉, 刘芳, 刘洋. 黄浦江河水及沉积物中抗生素抗性基因污染初步研究[C]. 第六届全国环境化学学术大会, 2011
15 Yu BH, Zakaria MP, Latif PA, Saari N. Occurrence of veterinary antibiotics and progesterone in broiler manure and agricultural soil in Malaysia [J]. Sci Total Environ, 2014, 488: 261-267
16 Awad YM, Kim SC, Abd El-Azeem SAM, Kim KH, Kim KR, Kim K, Jeon C, Lee SS, Ok YS. Veterinary antibiotics contamination in water, sediment, and soil near a swine manure composting facility [J]. Environ Earth Sci, 2014, 71 (3): 1433-1440
17 Zhang XX, Zhang T, Fang H. Antibiotic resistance genes in water environment [J]. Appl Microbiol Biotechnol, 2009, 82: 397-414
18 Peak N, Knapp CW, Yang RK, Hanfelt MM, Smith MS, Aga DS, Graham DW. Abundance of six tetracycline resistance genes in wastewater lagoons at cattle feedlots with different antibiotic use strategies [J]. Environ Microbiol, 2007, 9 (1): 143-151
19 何基兵, 胡安谊, 陈猛, 胡友彪, 于昌平. 九龙江河口及厦门污水处理设施抗生素抗性基因污染分析[J]. 微生物学通报, 2012, 39 (5): 683-695 [He JB, Hu AY, Chen M, Hu YB, Yu CP. Studies on the pollution levels of antibiotic resistance genes in Jiulong River estuary and wastewater treatment plants in Xiamen [J]. Microbiol China, 2012, 39 (5): 683-695]
20 杨颖. 北江水环境中抗生素抗性基因污染分析[D]. 广州: 中山大学, 2010 [Yang Y. Characterizing the pollution by the representative antibiotic resistance genes (ARGs) in the Beijiang River, South China [D]. Guangzhou: Sun Yat-sen University, 2010]
21 Knapp CW, Dolfing J, Ehlert PAI, Graham DW. Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940 [J]. Environ Sci Technol, 2010, 44 (2): 580-587
22 张俊, 杨晓洪, 葛峰, 王娜, 焦少俊, 叶波平. 长期施用四环素残留猪粪对土壤中耐药菌及抗性基因形成的影响[J]. 环境科学, 2014, 35 (6): 2374-2380 [Zhang J, Yang XH, Ge F, Wang N, Jiao SJ, Ye BP. Effects of long-term application of pig manure containing residual tetracycline on the formation of drug-resistant bacteria and resistance genes [J]. Environ Sci, 2014, 35 (6): 2374-2380]
23 Luo Y, Mao DQ, Rysz M, Zhou DX, Zhang HJ, Xu L, Alvarez PJJ. Trends in antibiotic resistance genes occurrence in the Haihe River, China [J]. Environ Sci Technol, 2010, 44 (19): 7220-7225
24 Heuer H, Kopmann C, Binh CTT, Top EM, Smalla K. Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G plus C content [J]. Environ Microbiol, 2009, 11 (4): 937-949
25 Munir M, Xagoraraki I. Levels of antibiotic resistance genes in manure, biosolids, and fertilized soil [J]. J Environ Qual, 2011, 40 (1): 248-255
26 D’Costa VM, King CE, Kalan L, Morar M, Sung WWL, Schwarz C, Froese D, Zazula G, Calmels F, Debruyne R, Golding GB, Poinar HN, Wright GD. Antibiotic resistance is ancient [J]. Nature, 2011, 477 (7365): 457-461
27 Gandolfi I, Franzetti A, Bertolini V, Gaspari E, Bestetti G. Antibiotic resistance in bacteria associated with coarse atmospheric particulate matter in an urban area [J]. J Appl Microbiol, 2011, 110 (6): 1612-1620
28 Huang XZ, Jonathan GF, Mohamad AC, Lashanda MG, Jukie AA, Su WW, Nikolich MP, Lesho EP. Characteristics of plasmids in multi-drug-resistant Enterobacteriaceae isolated during prospective surveillance of a newly opened hospital in Iraq [J]. PLoS ONE, 2012, 7 (7): 1-8
29 DoUiver H, Gupta S. Antibiotic losses in leaching and surface runoff from manureamended agricultural land [J]. J Environ Qual, 2008, 37 (3): 1227-1237
30 Xu J, Xu Y, Wang HM, Guo CS, Qiu HY, He Y, Zhang Y, Li XC, Meng W.Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its ef?uent-receiving river [J]. Chemosphere, 2015, 119: 1379-1385
31 沈群辉. 养殖场及周边农田土壤抗生素抗性基因和重金属污染初步研究[D]. 上海: 东华大学, 2013 [Shen QH. Preliminary study on the pollution levels of antibiotic resistance genes and heavy metals in feedlots and agricultural soils adjacent to feedlots in Shanghai, China [D]. Shanghai: Donghua University, 2013]
32 梁惜梅, 聂湘平, 施震. 珠江口典型水产养殖区抗生素抗性基因污染的初步研究[J]. 环境科学, 2013, 34 (10): 4073-4080 [Liang XM, Nie XP, Shi Z. Preliminary studies on the occurrence of antibiotic resistance genes in typical aquaculture area of the Pearl River Estuary [J]. Environ Sci, 2013, 34 (10): 4073-4080]
33 Forsberg KJ, Patel S, Gibson MK, Lauber CL, Knight R, Fierer N, Dantas G. Bacterial phylogeny structures soil resistomes across habitats [J]. Nature, 2014, 59: 612-616
34 Gautam D, Morten OAA, Rantimi DO, George MC. Bacteriar subsisting on antibiotics [J]. Science, 2008, 4: 100-103
35 Thomas CM, Nielsen KM. Mechanisms of, and barriers to, horizontal gene transfer between bacteria [J]. Nat Rev Microbiol, 2005, 3 (9): 711-721
36 Barlow M. What antimicrobial resistance has taught us about horizontal gene transfer [J]. Methods Mol Biol, 2009, 532: 397-411
37 张婉茹, 那广水, 陆紫皓, 高会, 李瑞婧, 吴限, 祖国仁, 姚子伟. 北黄海近岸海域磺胺类抗生素及其抗性Escherichia coli分布[J]. 应用与环境生物学报, 2014, 20 (3): 401-406 [Zhang WR, Na GS, Lu ZH, Gao H, Li RJ, Wu X, Zu GR, Yao ZW. Distribution of sulfonamides and sulfonamide-resistant Escherichia coli in the coastal marine environment of northern Yellow Sea, China [J]. Chin J Appl Environ Biol, 2014, 20 (3): 401-406]
38 Pei R, Kim SC, Carlson KH, Pruden A. Effect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG) [J]. Water Res, 2006, 40 (12): 2427-2435
39 Yang H, Byelashov OA, Geornaras I, Goodridge LD, Nightingale KK, Belk KE, Smith GC, Sofos JN. Presence of antibiotic resistant commensal bacteria in samples from agricultural, city, and national park environments evaluated by standard culture and real-time PCR methods [J]. Can J Microbiol, 2010, 56 (9): 761-770
40 祁彦洁, 刘菲. 地下水中抗生素污染检测分析研究进展[J]. 岩矿测试, 2014, 33 (1): 1-11 [Qi YJ, Liu F. Analysis of antibiotics in groundwater: a review [J]. Rock Miner Anal, 2014, 33 (1): 1-11]
41 Maki T, Hasegawa H, Kitami H, Fumoto K, Munekage Y, Ueda K. Bacterial degradation of antibiotic residues in marine fish farm sediments of Uranouchi Bay and phylogenetic analysis of antibiotic-degrading bacteria using 16S rDNA sequences [J]. Fish Sci, 2006, 72 (4): 811-820
42 Ali SW, Li R, Zhou WY, Sun JQ, Guo P, Ma JP, Li SP. Isolation and characterization of an abamectin-degrading Burkholderia cepacia-like GB-01 strain [J]. Biodegradation, 2010, 21 (3): 441-452
43 李圆杏, 黄宏, 刘臻. 模拟日光照射下三种抗生素的光降解行为[J]. 环境化学, 2013, 32 (8): 1513-1517 [Li YX, Huang H, Liu Z. Photodegradation behavior of three antibiotics with solar simulator [J]. Environ Chem, 2013, 32 (8): 1513-1517]
44 宋晨怡, 尹大强. 四环素光催化降解特性与选择性研究[J]. 环境科学, 2014, 35 (2): 619-625 [Song CY, Yin DQ. Characteristics and selectivity of photocatalytic-degradation of tetracycline hydrochloride [J]. Environ Sci, 2014, 35 (2): 619-625]
45 肖杰, 李静, 郑晓冬, 乔显亮. 4种表面活性剂对水中土霉素光解的影响[J]. 农业环境科学学报, 2013, 32 (7): 1451-1455 [Xiao J, Li J, Zheng XD, Qiao XL. Effects of surfactants on photodegradation of oxytetracycline in water [J]. J Agro-Environ Sci, 2013, 32 (7): 1451-1455]
46 Mitchella SM, Ullmanb JL, Teela AL, Watts RJ. pH and temperature effects on the hydrolysis of three β-lactam antibiotics: ampicillin, cefalotin and cefoxitin [J]. Sci Total Environ, 2014, 466: 547-555
47 Schlusener MP, Bester K. Persistence of antibiotics such as macrolides, tiamulin and salinomycin in soil [J]. Environ Pollut, 2006, 143 (3): 565-571
48 Naoko W, Thomas HH, Brian AB. Environmental occurrence and shallow ground water detection of the antibiotic monensin from dairy farms [J]. J Environ Qual, 2008, 37: 78-85
49 Le-Minh, N, Khan SJ, Drewes JE, Stuetz RM. Fate of antibiotics during municipal water recycling treatment processes [J]. Water Res, 2010, 44 (15): 4295-4323
50 Engemann CA, Adams L, Knapp CW, Graham DW. Disappearance of oxytetracycline resistance genes in aquatic systems [J]. FEMS Microbiol Lett, 2006, 263 (2): 176-182
51 韦蓓, 黄福义, 李虎, 苏建强. 污泥堆肥过程中磺胺类和大环内酯类抗性基因的残留[J]. 应用与环境生物学报, 2014, 20 (3): 395-400 [Wei B, Huang FY, Li H, Su JQ. Persistence of sulfonamide and macrolide resistance genes during sewage sludge composting [J]. Chin J Appl Environ Biol, 2014, 20 (3): 395-400]
52 Pei RT, Cha J, Carlson KH, Pruden A. Response of antibiotic resistance genes (ARG) to biological treatment in dairy lagoon water [J]. Environ Sci Technol, 2007, 41 (14): 5108-5113
53 Yang Y, Li B, Ju F, Zhang T. Exploring variation of antibiotic resistance genes in activated sludge over a four-year period through a metagenomic approach [J]. Environ Sci Technol, 2013, 47: 10197-10205
54 Brjesson S, Mattsson A, Lindgren PE. Genes encoding tetracycline resistance in a full-scale municipal wastewater treatment plant investigated during one year [J]. J Water Health, 2010, 8 (2): 247-256
55 Mckinney C, Ma YJ, Novak JT, Pruden A. Disinfection of microconstituent antibiotic resistance genes by UV light and sludge digestion [J]. Water Environ Fed, 2009, 20 (11): 577-589
56 Da Silva MF, Tiago I, Verissimo A, Boaventura RAR, Nunes OC, Manaia CM. Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant [J]. FEMS Microbiol Ecol, 2006, 55 (2): 322-329
57 Munir M, Wong K, Xagoraraki I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan [J]. Water Res, 2011, 45 (2): 681-693
58 Macauley JJ, Qiang ZM, Adams CD, Surampalli R, Mormile MR. Disinfection of swine wastewater using chlorine,ultraviolet light and ozone [J]. Water Res, 2006, 40 (10): 2017-2026
59 郑加玉. 养殖废水中抗生素及抗性基因在垂直流人工湿地中的去除[D]. 哈尔滨: 东北林业大学, 2013 [Zheng JY. Elimination of veterinary antibiotics and antibiotic resistance genes from swine wastewater in the vertical flow constructed wetlands [D]. Harbin: Northeast Forestry University, 2013]
60 Wu D, Huang ZT, Yang K, Graham D, Xie B. Relationships between antibiotics and antibiotic resistance gene levels in municipal solid waste leachates in Shanghai, China [J]. Environ Sci Technol, 2015, doi: 10. 1021/es506081z


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