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Correlation of the antioxidant property with the total phenolic content and total ?avonoids of different Dendrobium officinale extracts(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 03
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Correlation of the antioxidant property with the total phenolic content and total ?avonoids of different Dendrobium officinale extracts
HUANG Qin SHEN Yangxia ZHANG Chengjing LUO Aoxue FAN Yijun
Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China
Dendrobium officinale polyphenol ?avonoid antioxidant

This study aimed to understand the correlation of the antioxidant activity with the content of phenolics compounds and ?avonoids compounds in Dendrobium officinale in vitro. Four different polarity extracts (EE, CE, EAE and NBE) were prepared with different solvents. Antioxidant activity of the 4 samples was studied by reducing power assay, DPPH radical scavenging assay, ABTS radical scavenging assay and Hydroxyl radical scavenging assay with Vc as the positive control. The results indicated that CE had the highest content of phenolics compounds (40.96 mg/g) and ?avonoids compounds (104.48 mg/g). The antioxidant assays showed that CE had strong antioxidant ability on DPPH, ABTS and Hydroxyl radical. At 375 μg/mL, the scavenging rate of CE on ABTS got to 100% ( IC50 = 88.82 μg/mL). At 1 500 μg/mL, the scavenging effect on DPPH was 78.45% (IC50 = 394.62 μg/mL). The reducing power of CE was also strong, 62.29% at 1 500 μg/mL, and 100% (IC50 = 905.06 μg/mL) at 3 000 μg/mL. The results of correlation study showed that compared with the content of phenolics, the content of ?avonoids had a stronger relationship with the ABTS (r = 0.845), DPPH radical (r = 0.902) scavenging activity and the reducing power (r = 0.994), whereas the content of phenolics had a stronger relationship with Hydroxyl radical (r = 0.521) scavenging activity than the content of ?avonoids. This research indicated that there is a close relationship between the content of phenolics and ?avonoids and the antioxidant activity of all extracts, and the material basis of antioxidant activity of Dendrobium officinale may be phenolics and ?avonoids.


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Last Update: 2014-07-01