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Effect of multiple Benzo(a)pyrene addition on its bioavailability and coelomocyte lysosomes and chromosomes in earthworms(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 06
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Effect of multiple Benzo(a)pyrene addition on its bioavailability and coelomocyte lysosomes and chromosomes in earthworms
ZHANG Wei ZHENG Bin MA Jingjing GE Gaofei TAN Huarong GAO Hongjian
1School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China 2Biotechnology Center of Anhui Agricultural University, Hefei 230036, China
B[a]P superimposed pollution Tenax extract lysosome membrane micronucleus frequency

In order to understand Benzo(a)pyrene (B[a]P) bioavailability in soil and its accumulation in earthworms, it is necessary to study B[a]P fate in soil and its toxic effect on earthworms. This study investigated the effects of multiple and one-time addition of B[a]P in soil on its availability and the neutral red retention time (NRRT) as well as the micronucleus rates in earthworm coelomocytes. By using multiple and one-time addition methods, exposure experiments with different aging times of B[a]P (1, 7, 14, 28, and 56 days) were conducted to analyze the contents of B[a]P in soil by chemical solvent (accelerated solvent extraction, ASE) and Tenax-TA extractive methods. Earthworms were exposed to soils with different aging time of B[a]P. After exposure for 7 days, earthworms were collected for analysis of B[a]P enriched, neural red retention time and micronucleus rates in coelomocytes. The contents of ASE and Tenax extractable B[a]P in soil and the amounts of B[a]P enriched in earthworms sharply declined in the first 28 d (0-28 d), and then slowly decreased between 28 d and 56 d for both the multiple and one-time addition methods. The micronucleus rates in earthworms sharply declined, while the NRRT in earthworm coelomocytes greatly increased in the first stage, and then slowly decreased in the following incubation time for both addition methods. The results showed a very significantly positive correlation (R2 = 0.8614, P < 0.01) between the amounts of B[a]P accumulated in earthworms and the contents of B[a]P extracted from soil by Tenax. The available contents of B[a]P in soil and the amounts of B[a]P enriched in earthworms in multiple addition were 26.00% and 36.04% lower than that in the one-time pollution treatment. The NRRT and micronucleus rate in earthworm coelomocytes in the multiple addition was 13.00% higher and 36.10% lower than those in the one-time addition, respectively. To sum up, this study provides important data for assessing ecological toxicity of multiple addition of B[a]P in soil, showing that the soil available B[a]P and its toxicity to earthworms are lowered by multiple addition.


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