|Table of Contents|

Effect of multiple Benzo(a)pyrene addition on its bioavailability and coelomocyte lysosomes and chromosomes in earthworms(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 06
Page:
1020-1026
Research Field:
Articles
Publishing date:

Info

Title:
Effect of multiple Benzo(a)pyrene addition on its bioavailability and coelomocyte lysosomes and chromosomes in earthworms
Author(s):
ZHANG Wei ZHENG Bin MA Jingjing GE Gaofei TAN Huarong GAO Hongjian
1School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China 2Biotechnology Center of Anhui Agricultural University, Hefei 230036, China
Keywords:
B[a]P superimposed pollution Tenax extract lysosome membrane micronucleus frequency
CLC:
X171.5
PACS:
DOI:
10.3724/SP.J.1145.2014.04002
DocumentCode:

Abstract:
In order to understand Benzo(a)pyrene (B[a]P) bioavailability in soil and its accumulation in earthworms, it is necessary to study B[a]P fate in soil and its toxic effect on earthworms. This study investigated the effects of multiple and one-time addition of B[a]P in soil on its availability and the neutral red retention time (NRRT) as well as the micronucleus rates in earthworm coelomocytes. By using multiple and one-time addition methods, exposure experiments with different aging times of B[a]P (1, 7, 14, 28, and 56 days) were conducted to analyze the contents of B[a]P in soil by chemical solvent (accelerated solvent extraction, ASE) and Tenax-TA extractive methods. Earthworms were exposed to soils with different aging time of B[a]P. After exposure for 7 days, earthworms were collected for analysis of B[a]P enriched, neural red retention time and micronucleus rates in coelomocytes. The contents of ASE and Tenax extractable B[a]P in soil and the amounts of B[a]P enriched in earthworms sharply declined in the first 28 d (0-28 d), and then slowly decreased between 28 d and 56 d for both the multiple and one-time addition methods. The micronucleus rates in earthworms sharply declined, while the NRRT in earthworm coelomocytes greatly increased in the first stage, and then slowly decreased in the following incubation time for both addition methods. The results showed a very significantly positive correlation (R2 = 0.8614, P < 0.01) between the amounts of B[a]P accumulated in earthworms and the contents of B[a]P extracted from soil by Tenax. The available contents of B[a]P in soil and the amounts of B[a]P enriched in earthworms in multiple addition were 26.00% and 36.04% lower than that in the one-time pollution treatment. The NRRT and micronucleus rate in earthworm coelomocytes in the multiple addition was 13.00% higher and 36.10% lower than those in the one-time addition, respectively. To sum up, this study provides important data for assessing ecological toxicity of multiple addition of B[a]P in soil, showing that the soil available B[a]P and its toxicity to earthworms are lowered by multiple addition.

References

1 Marcello Z, Claudia C, Jose VT. Probabilistic ecological risk assessment of 1,2,4-trichloro benzene at a former industrial contaminated site [J]. Environ Sci Technol, 2005, 39 (9): 2920-2926
2 Bull S, Collins C. Promoting the use of BaP as a marker for PAH exposure in UK soils [J]. Environ Geochem Health, 2013, 35 (1): 101-109
3 盛下放, 何琳燕, 胡凌飞. 苯并[a]芘降解菌的分离筛选及其降解条件的研究[J]. 环境科学学报, 2005, 25 (6): 791-795 [Sheng XF, He LY, Hu LF. Isolation of a benzo[a]pyrene-degrading strain and its degradation conditions [J]. Acta Sci Cir, 2005, 25 (6): 791-795]
4 黄健, 李春玉, 刘济, 石利利, 姚成. 生物堆模拟法修复苯并[a]芘和二苯并[a,h]蒽污染土壤[J]. 生态与农村环境学报, 2012, 28 (2): 221-224 [Huang J, Li CY, Liu JL, Shi LL, Yao C. Simulated bioremediation of BaP& DBA contaminated soil [J]. J Ecol Rural Environ, 2012, 28 (2): 221-224]
5 郜红建, 蒋新, 王芳, 余贵芬, 卞永荣. DDTs 在土壤中的老化规律及生物有效性[J]. 土壤学报, 2007, 44 (1): 79-83 [Gao HJ, Jiang X, Wang F, Yu GF, Bian YR. Aging and bioavailability of DDTs in soil [J]. Acta Pedol Sin, 2007, 44 (1): 79-83]
6 Zhang H, Zhao FJ, Sun B. A new method to measure effective soil solution concentration predicts copper availability to plants [J]. Environ Sci Technol, 2001, 35 (12): 2602-2607
7 林纪旺. 土壤中多环芳烃生物有效性研究进展[J]. 安徽农学通报, 2011, 17 (8): 34-37 [Lin JW. Research advances in bioavailability of polycyclic aromatic hydrocarbons in soil [J]. Anhui Agri Sci Bul, 2011, 17 (8): 34-37]
8 Reid BJ, Jones KC, Semple KT. Bioavailability of persistent organic pollutants in soils and sediments—a perspective on mechanisms, consequences and assessment [J]. Environ Pollut, 2000, 108 (1): 103-112
9 Bergknut M, Kitti A, Lundstedt S, Tysklind M, Haglund P. Assessment of the availability of polycyclic aromatic hydrocarbons from gasworks soil using different extraction solvents and techniques [J]. Environ Toxicol Chem, 2004, 23 (8): 1861-1866
10 Tao S, Xu F, Liu W, Cui Y, Coveney RM. A chemical extraction method for mimicking bioavailability of polycyclic aromatic hydrocarbons to wheat grown in soils containing various amounts of organic matter [J]. Environ Sci Technol, 2006, 40 (7): 2219-2224
11 Cornelissen G, Rigterink H, Hulscher DEM, Paul CM. A simple Tenax? extraction method to determine the availability of sediment‐sorbed organic compounds [J]. Environ Toxicol Chem, 2001, 20 (4): 706-711
12 Li M, Liu Z, Xu Yb. Comparative effects of Cd and Pb on biochemical response and DNA damage in the earthworm (Eisenia fetida) (Annelida, Oligochaeta) [J]. Chemosphere, 2009, 74 (5): 621-625
13 刘德鸿, 成杰民, 刘德辉. 蚯蚓对土壤中铜、镉形态及高丹草生物有效性的影响[J]. 应用与环境生物学报, 2007, 13 (2): 209- 217 [Liu DH, Cheng JM, Liu DH. Effect of earthworm on Cu and Cd forms and their availability to sorghum bioavailability sudanense [J]. Chin J Appl Environ Biol, 2007, 13 (2): 209-217]
14 OECD. Earthworm reproduction test (Eisenia fetida/Eiseniaandrei) [R]. Guidelines for Testing of Chemicals, 2004, No. 222
15 ISO. Soil quality - biological methods - effects of pollutants on soil fauna - determination of effects on reproduction on earthworms (Eisenia fetida) [S]. British Standards Institution, 1998, No. 11268-2
16 Eason CT, Svendsen CO, Halloran K, Weeks JM. An assessment of the lysosomal neutral red retention test and immune function assay in earthworms (Eiseniaandrei) following exposure to chlorpyrifos, benzo-a-pyrene (BaP), and contaminated soil [J]. Pedobiologia, 1999, 43 (6): 641-645
17 Giggleman MA, Fitzpatrick LC, Goven AJ. Effects of pentachlorophenol on survival of earthworms (Lumbricus terrestris) and phagocytosis by their immunoactive coelomocytes [J]. Environ Toxicol Chem, 1998, 17 (12): 2391-2394
18 褚金龙, 高红霞, 刘英莉, 关维俊, 范建军, 王秀英, 赵雅玲, 马冬. 用彗星实验和微核实验反映土壤有机污染物的联合毒性[J]. 现代预防医学, 2012, 39 (17): 4497-4499 [Chu JL, Gao HX, Liu YL, Guan WJ, Fan JJ, Wang XY, Zhao YL, Ma D. Comet assay and micronucleus test in detection of joint toxicity of organic pollutants in soil [J]. Mod Preve Med, 2012, 39 (17): 4497-4499]
19 刘瑞民, 王学军. 土壤多环芳烃污染的地统计学研究进展[J]. 中国环境监测, 2004, 20 (4): 61-66 [Liu RM, Wang XJ. Reviews on the pollution research of polycyclic aromatic hydrocarbons in soil using geostatistics [J]. Environ Monit Chin, 2004, 20 (4): 61-66]
20 鲍士旦. 土壤与农化分析[M]. 3版. 北京: 中国农业出版社, 2010. 25-114 [Bao SD. Soil and Agricultural Chemistry Analysis [M]. 3rd ed. Beijing: China Agriculture Press, 2010]
21 郑彬, 马静静, 葛高飞, 郜红建. B[a]P累积污染对土壤呼吸强度的影响[J]. 农业环境科学学报, 2013, 32 (2): 259-266 [Zheng B, Ma JJ, Ge GF, Gao HJ. Influences of Benzo(a)pyrene superimposed pollution on soil respiration intensity [J]. J Agro-Environ Sci, 2013, 32 (2): 259-266]
22 马静静, 郑彬, 张伟, 葛高飞, 郜红建. 土壤B[a]P叠加污染对蚯蚓体腔细胞SOD, POD和MDA的毒性效应[J]. 生态与农村环境学报, 2013, 29: 743-748 [Ma JJ, Zhen B, Zhang W, Ge GF, Gao HJ. Toxic effects of repeated superimposed B[a]P pollution of soil on SOD, POD and MDA in coelomocytes of Eisenia fetida [J]. J Ecol Rural Environ, 2013, 29: 743-748]
23 Gao YZ, Xiong W, Ling WT, Wang XR, Li QL. Impact of exotic and inherent dissolved organic matter on sorption of phenanthrene by soils [J]. J Hazard Mater, 2007, 140 (1-2): 138-144
24 Sverdrup LE, Jensen J, Krogh PH, Stenersen J. Studies on the effect of soil aging on the toxicity of pyrene and phenanthrene to a soil-dwelling springtail [J]. Environ Toxicol Chem ,2002, 21 (3): 489-492
25 高学晟, 姜霞, 区自清. 多环芳烃在土壤中的行为[J]. 应用生态学报, 2002, 13 (4): 501-504 [Gao XS, Jiang X, Ou ZQ. Behaviors of polycyclic aromatic hydrocarbons (PAHs) in soils [J]. Chin J Appl Ecol, 2002, 13 (4): 501-504]
26 Hatzinger PB, Alexander M. Effect of aging of chemicals in soil on their biodegradability and extractability [J]. Environ Sci Technol, 1995, 29 (2): 537-545
27 Yang Y, Zhang N, Xue M, Tao S. Impact of soil organic matter on the distribution of polycyclic aromatic hydrocarbons (PAHs) in soils [J]. Environ Pollut, 2010, 158 (6): 2170-2174
28 Alexander M. Aging, bioavailability and overestimation of risk from environmental pollutants [J]. Environ Sci Technol, 2000, 34 (20): 4259-4265
29 张聪恺, Muhammad IK, Sardar AC, 沈超峰, 唐先进, 申开丽, 陈蕾, 陈新才, 施积炎, 陈英旭. 温和提取法评估老化前后土壤中菲的生物有效性[J]. 土壤学报, 2011, 48 (1): 78-82 [Zhang CK, Muhammad IK, Sardar AC, Sheng CF, Tang XJ, Shen KL, Chen L, Chen XC, Shi JY, Chen YX. Assessment of Phenanthrene bioavailability in soils before and after aging with mild extraction method [J]. Acta Pedol Sin, 2011, 48 (1): 78-82]
30 Kukkonen JVK, Landrum PF, Mitra S. The role of desorption for describing the bioavailability of select polycyclic aromatic hydrocarbon and polychlorinated biphenyl congeners for seven laboratory‐spiked sediments [J]. Environ Toxicol Chem, 2004, 23 (8): 1842-1851
31 张薇, 宋玉芳, 孙铁珩, Ackland ML, Belogolova G. 土壤低剂量芘污染对蚯蚓若干生化指标的影响[J]. 应用生态学报, 2007, 18 (9): 2097-2103 [Zhang W, Song YF, Sun TH, Ackland ML, Belogolova G. Effects of low dosage pyrene pollution on biochemical characters of earthworm (Eisenia fetida) in soil [J]. Chi J Appl Ecol, 2007, 18 (9): 2097-2103

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Last Update: 2015-01-05