|Table of Contents|

 Bacterial community structure analysis for mushroom (Agaricus bisporus) compost using PCR-DGGE technique(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 05
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 Bacterial community structure analysis for mushroom (Agaricus bisporus) compost using PCR-DGGE technique
 GUO Yaping ZHANG Guoqing CHEN Qingjun YANG Kai
1College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China
2College of Biological Sciences and Engineering, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
 PCR-DGGE Agaricus bisporus compost bacterial community
S141.4 : Q938

This study aimed to investigate the bacterial communities in mushroom compost using straw, corn stalks, and cow dung as major compost matrix. Bacterial communities of samples at the beginning of composting, at the end of fermentation phase I and II were collected and analyzed using Polymerase Chain Reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) method based on 16S rDNA universal primers from Escherichia coli. A total of 56 different clone sequences were obtained (GenBank accession numbers of KF630598-KF630653). They were classified into 7 phyla and 43 genus. Dominant microflora during composting belonged to phyla Proteobacteria, Firmicutes, and Actinobacteria, with the dominant genus of Bacillus, Paenibacillus, Thermomonospora, Thermasporomyces, Pseudomonas, and Cellvibrio. Bacterial diversity (Shannon index) analysis showed that bacterial species in straw treatment continuously increased during composting, while those in corn stalks treatment firstly increased and then reduced. Principal component analysis showed that corn stalk compost at the end of fermentation phase I and phase II were clustered into one group, suggesting that corn stalk composts faster than anticipated. In general, straw compost has higher bacterial diversity but longer composting time, while corn stalk compost has lower bacterial diversity but shorter composting time.



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Last Update: 2014-10-30