|Table of Contents|

 Bacterial community structure analysis for mushroom (Agaricus bisporus) compost using PCR-DGGE technique(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 05
Page:
832-839
Research Field:
Articles
Publishing date:

Info

Title:
 Bacterial community structure analysis for mushroom (Agaricus bisporus) compost using PCR-DGGE technique
Author(s):
 GUO Yaping ZHANG Guoqing CHEN Qingjun YANG Kai
1College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China
2College of Biological Sciences and Engineering, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
Keywords:
 PCR-DGGE Agaricus bisporus compost bacterial community
CLC:
S141.4 : Q938
PACS:
DOI:
10.3724/SP.J.1145.2014.03020
DocumentCode:

Abstract:
This study aimed to investigate the bacterial communities in mushroom compost using straw, corn stalks, and cow dung as major compost matrix. Bacterial communities of samples at the beginning of composting, at the end of fermentation phase I and II were collected and analyzed using Polymerase Chain Reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) method based on 16S rDNA universal primers from Escherichia coli. A total of 56 different clone sequences were obtained (GenBank accession numbers of KF630598-KF630653). They were classified into 7 phyla and 43 genus. Dominant microflora during composting belonged to phyla Proteobacteria, Firmicutes, and Actinobacteria, with the dominant genus of Bacillus, Paenibacillus, Thermomonospora, Thermasporomyces, Pseudomonas, and Cellvibrio. Bacterial diversity (Shannon index) analysis showed that bacterial species in straw treatment continuously increased during composting, while those in corn stalks treatment firstly increased and then reduced. Principal component analysis showed that corn stalk compost at the end of fermentation phase I and phase II were clustered into one group, suggesting that corn stalk composts faster than anticipated. In general, straw compost has higher bacterial diversity but longer composting time, while corn stalk compost has lower bacterial diversity but shorter composting time.

References

 

1 王德芝, 刘瑞芳, 马兰, 林向群, 张淑霞. 现代食用菌生产技术[M]. 武汉: 华中科技大学出版社, 2012 [Wang DZ, Liu RF, Ma L, Lin XQ, Zhang SX. Modern Mushroom Production Technology [M]. Wuhan: Huazhong University of Science and Technology Press, 2012]<br/>

2 许修宏, 李洪涛, 张迪. 堆肥微生物学原理及双孢蘑菇栽培[M]. 北京: 科学出版社, 2009 [Xu XH, Li HT, Zhang D. Compost Microbiology Principles and Agaricus bisporus Cultivation [M]. Beijing: Science Press, 2009]<br/>

3 Sharma SS, Yons GL, Chambers J. Comparison of the changes in mushroom (Agaricus bisporus) compost during windrow and bunker stages of phase I and [J]. Assoc Appl Biol, 2000, 136: 59-68<br/>

4 Wagner M, Amann R, Lemmer H, Schleifer KH. Probing activated sludge with oligonucleotides specific for Proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure[J]. Appl Environ Microbiol, 1993, 59: 1520-1525<br/>

5 Gerard M, Kornelia S. Application of denaturing gradientgel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology [J]. Anton Leeuw, 1998, 73: 127-141<br/>

6 Novinscak A, Nadine JD, Celine S, Martin F. Characterization of bacterial and fungal communities in composted biosolids over a 2 year period using denaturing gradient gel electrophoresis [J]. Can J Microbiol, 2009, 55: 375-387<br/>

7 Wu L, Li F, Deng CY, Xu DQ, Jiang SW, Xiong YZ. Amethod for obtaining DNA from compost[J]. Appl Microbiol Biotechnol, 2009, 84: 389-395<br/>

8 Zeng GM, Yu Z, Chen YN, Zhang JC, Li H, Yu M , Zhao MJ. Response of compost maturity and microbial community composition to pentachlorophenol (PCP)-contaminated soil during composting [J]. Bioresource Technol, 2011, 102: 5905-5911 <br/>

9 王小芬, 王伟东, 高丽娟, 崔宗均. 变性梯度凝胶电泳在环境微生物研究中的应用详解[J]. 中国农业大学学报, 2006, 11 (5): 1-7 [Wang XF, Wang WD, Gao LJ, Cui ZJ. Protocols of applification of denaturing gradient gel electrophoresis (DGGE) in studies of environmental microorganism [J]. J China Agric Univ, 2006, 11 (5): 1-7]<br/>

10 Raquel B, Felícitas V, Antoni S. Dehydrogenase activity as a method for monitoring the composting process [J]. Bioresource Technol, 2008, 99: 905-908<br/>

11 何丽鸿, 陈明杰, 潘迎捷. 采用变性梯度凝胶电泳研究双孢蘑菇培养料后发酵过程中的细菌群落结构[J]. 微生物学报, 2009, 49 (2): 227-232 [He LH, Chen MJ, Pan YJ. Bacterial communities in the phase of Agaricus bisporus compost by denaturing gradient gel electrophoresis [J]. Acta Microbiol Sin, 2009, 49 (2): 227-232]<br/>

12 Székely AJ, Sipos R, Berta B, Vajna B, Hajdú C. DGGE and T-RFLP analysis of bacterial succession during mushroom compost production and sequence-aided T-RFLP profile of mature compost [J]. Microbial Ecol, 2009, 57: 522-533<br/>

13 贾洋洋. 利用宏基因组方法分析堆肥生境中微生物区系的变化[D]. 济南: 山东大学, 2012 [Jia YY. Microbial diversity analysis in composting environment by metagenomic method [D]. Jinan: Shandong University, 2012]<br/>

14 Xiao Y, Zeng GM, Yang ZH, Ma YH, Huang C, Shi WJ, Xu ZY, Huang J, Fan CZ. Effects of continuous thermophilic composting (CTC) on bacterial community in the active composting process [J]. Environ Microbiol, 2011, 62: 599-608<br/>

15 Agnolucci M, Cristani C, Battini F, Palla M. Microbially-enhanced composting of olive mill solid waste (wet husk): bacterial and fungal community dynamics at industrial pilot and farm level [J]. Bioresource Technol, 2013, 134: 10-16<br/>

16 席北斗, 刘鸿亮, 白庆中, 黄国, 曾光明, 李英军. 堆肥中纤维素和木质素的生物降解研究现状[J]. 环境污染治理技术与设备, 2002, 3 (3): 19-23 [Xi BD, Liu HL, Bai QZ, Huang GH, Zeng GM, Li YJ. Study on current status of lignin and cellulose biodegradation in composting process [J]. Techniq Equip Environ Pollut Control, 2002, 3 (3): 19-23]<br/>

17 Keiko W, Norio N, Tatsuki T, Norio K. The dominant bacteria shifted from the order ‘‘Lactobacillales’’ to Bacillales and Actinomycetales during a start-up period of large-scale, completely-mixed composting reactor using plastic bottle flakes as bulking agent [J]. World J Microbiol Biotechnol, 2009, 25: 803-811<br/>

18 王鸿磊, 王红艳, 宋俊芬, 丁强, 徐康铭, 邹积华. 双孢菇培养料工厂化发酵过程中微生物及物质变化研究[J]. 安徽农业科学, 2011, 39 (1): 94-96 [Wang HL, Wang HY, Song JF, Ding Q, Xu KM, Zhou JH. Variation of microorganisms and physicochemical properties of Agaricus bisporus compost [J]. J Anhui Agric, 2011, 39 (1): 94-96]<br/>

19 刘佳, 李婉, 许修宏, 李洪涛. 接种纤维素降解菌对牛粪堆肥微生物群落的影响[J]. 环境科学, 2011, 32 (10): 3073-3081 [Liu J, Li W, Xu XH, Li HT. Effect of cellulose-decomposing strain on microbial community of cow manure compost [J]. Environ Sci, 2011, 32 (10): 3073-3081]<br/>

20 姜成, 王泽生. 蘑菇培养料堆制过程中微生物的演替及作用[J]. 微生物学杂志, 2003, 23 (1): 56-58 [Jiang C, Wang ZS. The development and functions of microorganisms in the composting process for Agaricus bisporus cultivation [J]. J Microbiol, 2003, 23 (1): 56-58]<br/>

21 Gerben S, Tineke W, Olijnsm A, Gerrits PG. Inoculation of Scytalidium thermophilum in button mushroom compost and its effect on yield [J]. Appl Environ Microbial, 1994, 60 (9): 3049-3054<br/>

22 Wiegant WM. Growth characteristics of the thermophilic fungus Scytalidium thermophilum in relation to production of mushroom compost [J]. Appl Environ Microbial, 1992, 58: 1301-1307<br/>

23 Zarenejad F, Yakhchali B, Rasooli I. Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production [J]. World J Microbiol Biotechnol, 2012, 28 (1): 99-104<br/>

24 吴小建. 嗜热真菌的分离鉴定及其在双孢蘑菇栽培中的应用研究[D]. 南宁: 广西大学, 2012 [Wu XJ. Isolation and identification of thermophilic fungi and cultivation of Agaricus bisporus on substrates pre-colonized by Scytaidum thermophilum [D]. Nanning: Guangxi University, 2012]<br/>

25 席北斗, 党秋玲, 魏自民, 李鸣晓, 夏训峰, 杨天学, 陈凤先. 生活垃圾化堆肥对放线菌群落的影响[J]. 农业工程学报, 2011, 27: 228-23 [Xi BD, Dang QL, Wei ZM, Li MX, Xia XF, Yang TX, Chen FX. Effects of microbial inoculants on actinomycetes communities diversity during municipal solid waste composting [J]. Trans CSAE, 2011, 27: 228-223]<br/>

26 王晶, 许修宏. 利用PCR-DGGE方法研究添加复合菌剂对堆肥微生物群落的影响[J]. 农业环境科学学报, 2011, 30 (12): 2602-2607 [Wang J, Xu XH. Effects of adding to complex bacteria on microbial community of compost assessed by PCR-DGGE techniques [J]. J Agro-Environ Sci, 2011, 30 (12): 2602-2607]

Memo

Memo:
-
Last Update: 2014-10-30