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Characterization of reduction of hexavalent chromium by Exiguobacterium sp. MH3(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 05
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Characterization of reduction of hexavalent chromium by Exiguobacterium sp. MH3
ZHANG Ying ZHANG Yingying TANG Jie MA Jiong
School of Environment and Energy, Peking University Shenzhen, Shenzhen 518055, China
Exiguobacterium Cr(VI) reduction additional carbon sources coexisting ion wastewater treatment

The hexavalent chromium [Cr(VI)] is the most toxic and soluble form of chromium, thus the main contaminant. The reduction of Cr(VI) to the much less toxic Cr(III) by microorganisms is an economical and effective method to treat Cr(VI). In this work, we studied the ability and characteristics of Exiguobacterium sp. MH3 isolated from the rhizosphere of a duckweed Lemna minor in Cr(VI) reduction. We measured the tolerance of MH3 to Cr(VI) in LB or M9 medium; the removal rate of Cr(VI) was analyzed in LB medium under different Cr(VI) concentrations. The concentrations of total Cr and Cr(VI) were measured to determine the mechanism of Cr(VI) removal. In addition, we studied the effects of pH, additional carbon sources, and coexisting ions on the reduction of Cr(VI). Exiguobacterium sp. MH3 was able to completely remove 5 mg L-1 Cr(VI) in LB medium in 12 h, and 15 mg L-1 in 36 h. The reaction was mostly due to reduction of Cr(VI) since the total concentration of Cr was stable with Cr(VI) decreasing. Both the supernatant of the culture and the extraction of MH3 showed reduction of Cr(VI). The optimal pH of the reduction was determined as 7.0. Extra carbon sources including mannitol, glucose, and sucrose could promote Cr(VI) reduction, so could Cu2+. In contrast, Zn2+, Mn2+, and Cd2+ inhibited the growth of MH3 and reduction of Cr(VI). PO43- was also able to promote the growth of MH3 and the removal of Cr(VI). These results indicated that Exiguobacterium sp. MH3 removes Cr(VI) with very little biosorption of Cr by reduction, most probably through secreted enzymes. It is an effective Cr(VI) reducing bacterium, and should have great potential in treating Cr(VI)-containing wastewaters, particularly in settings with additional carbon source and/or coexisting Cu2+.


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Last Update: 2014-10-29