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Research progress of viable but non-culturable state of aquatic bacteria(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 06
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Research progress of viable but non-culturable state of aquatic bacteria
DI Congcong HU Ping HU Zhangli CHEN Huirong
Shenzhen Key Laboratory of Marine Bioresource and Eco–environmental Science, Shenzhen Engineering Laboratory of Marine Algal Biotechnology, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
induction stress resuscitation promoting factors dormancey

Viable but non-culturable (VBNC) state is a dormant state of non-spore-forming bacteria under environmental stress. Cells under VBNC state are still alive with low levels of metabolic activity, but lose their ability to form colonies on growth medium. The reverse process is called resuscitation during which the cells regain culturability and form colonies. Since the first report of VBNC state bacteria in 1982, over 60 non-spore-forming bacterial species have been found capable of entering VBNC state, including several human pathogens. As cells under VBNC state in water and food cannot be detected by traditional methods based on plate counting, it is crucial to reassess the current viability detection methods for the concern of public health. Although lots of reports about VBNC state cells are published every year, no general model has been developed in terms of the VBNC induction and resuscitation mechanism. This review summarizes the current findings on VBNC induction and resuscitation process, the biological characteristics and possible genetic mechanisms of VBNC bacteria, especially the correlations between VBNC state and quorum sensing and general stress response σ factors. It is suggested that VBNC state is a universal mechanism utilized by microorganisms and that cell-to-cell communication plays an important role in this process. In the end, the review puts forward suggestions on development for future researches in this field,in order to provide new insights for future study of VBNC bacteria in aquatic ecosystem.


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