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 Effect of Vitamin C, Vitamin E and Glutamine compound nutrients on serum biochemical parmeters and expression of HSP70 for anti-cold stress in Wistar Rats(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 05
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 Effect of Vitamin C, Vitamin E and Glutamine compound nutrients on serum biochemical parmeters and expression of HSP70 for anti-cold stress in Wistar Rats
 LIU Cong LI Jian LI Changsheng JI Hong GUO Jingru LI Peng YANG Huanmin LI Shize
 1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
 compound nutrients anti-cold stress rats serum biochemical indicators HSP70

This study aimed to understand the effect of VC, VE and Glutamine (Gln) addition in diet on the cold stress resistance of rats. A cold-stress model was first established on 30 clean female Wistar rats randomly divided into three groups (N = 10). The experimental group was intragastricly administered a mixture of VC, VE and Gln (VC 600 mg/kg, VE 100 mg/kg and Gln 1 000 mg/kg); the cold stress group were exposed to the temperature of 11±1 oC for 24 h after model establishment; and the normal temperature group were kept under temperature of 21±1 oC. The concentration of antioxidant parameters in serum (NE, GSH-PX, SOD, T-AOC, MDA) were detected by ELISA technology. The relative expression of HSP70 mRNA was determined by method of qRT-PCR. The results indicated that: (1) the concentration of NE in serum tended to significantly increase after 24 h of cold stress (P < 0.01); (2) the concentration of antioxidants in serum tended to significantly reduce after 24 h of cold stress (P < 0.01); (3) the HSP70 mRNA level of q-PCR test of the cold stress group was extremely significantly higher than that of the normal temperature group (P < 0.01). From the above results, it could be concluded that the compound nutrients additive of VC, VE and Gln has a good effect on anti-cold stress of Wistar rats, with the optimal dose in basal diets as VC 600 mg/kg ,VE 100 mg/kg and Gln 1 000 mg/kg.



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Last Update: 2014-10-30