|Table of Contents|

Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2015 01
Page:
170-174
Research Field:
Communications
Publishing date:

Info

Title:
Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation
Author(s):
ZHAO Xingbing WU Weijia XIAO Nenqun ZHANG Hualing TAN Zhoujin CAI Guangxian
1Hunan University of TCM, Changsha 410208, China 2Hunan Engineering Research Center of Precious and Endangered Medicinal Materials, Changsha 410007, China
Keywords:
Spleen-deficiency constipation ultra-micro powder of traditional Chinese medicine Dendrobium officinale molecular diversity of intestinal bacteria ARDRA
CLC:
R285.5
PACS:
DOI:
10.3724/SP.J.1145.2013.12035
DocumentCode:

Abstract:
This research aimed to study the effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation, in order to provide scientific basis for ultra-micro D. officinale therapy. The mice with spleen-deficiency constipation in treatment groups were respectively given the traditional decoction of D. officinale and 50% dose ultra-micro powder D. officinale. The metagenome DNA of intestinal flora was extracted from intestinal contents. Then ARDRA analysis was made after PCR by universal primer of bacteria. The results showed that OTUs, Shannon index and Brillouin index were the same between the normal group and the 50% dose ultra-micro powder group, and between the model group and the traditional decoction group, but greater in the former two groups than the latter ones. The similarity coefficient (0.500 0) was bigger between the 50% dose ultra-micro powder group and the normal group than between the model group and the traditional decoction group, which was 0.181 8. Both cluster analysis and principal components analysis showed that the molecular diversity of intestinal microbiotia in mice of 50% dose ultra-micro powder group was the closest to that in the normal group. The results showed that the weakened molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation can be regulated by the two kinds of D. officinale treatments to different extent. The efficacy of 50% dose ultra-micro powder D. officinale is better than the traditional decoction.

References

1 Fernández-Ba?ares F. Nutritional care of the patient with constipation [J]. Nutr Dietary Consequences Gastronintestinal Dis, 2006, 20 (3): 575 -587
2 罗迪, 庞璐, 赵兴兵, 蔡光先, 吴维佳. 浅析《本草纲目》中铁皮石斛的功能主治[J]. 中国中医杂志, 2013, 29 (3): 108-109 [Luo D, Pang L, Zhao XB, Cai GX, Wu WJ. The indications of Dendrobium officinale on the “compendium of materia medica” [J]. Hunan J Trad Chin, 2013, 29 (3): 108-109]
3 王立明, 徐建华, 陈立钻, 孙继军, 吴洪海. 铁皮枫斗晶对实验性胃阴虚证的药效学研究[J]. 中成药, 2002, 10 (24): 803-805 [Wang LM, Xu JH, Chen LZ, Sun JJ, Wu HH. Pharmacodynamics of Dendrobium candicum granules on experimental stomach Yin deficiency [J]. Chin Trad Patent Med, 2002, 10 (24): 803-805]
4 Davey ME, O’toole GA. Microbial biofilms: from ecology to molecular genetics [J]. Microbiol Mol Biol Rev, 2000, 64: 847-867
5 Hooper LV, Wong MH, Thelin A. Molecu-lar analysis of commensal host-bacterial relationships in the intestine [J]. Science, 2001, 291: 881-884
6 武娜, 朱宝利. 人体肠道微生物与健康[J]. 科学(上海), 2013, 65 (2): 13-16 [Wu N, Zhu BL. Human gut microbes and health [J]. Science (Shanghai), 2013, 65 (2): 13-16]
7 翁幸鐾, 糜祖煌. 人体肠道微生物群落与疾病[J]. 公共卫生与临床医学, 2010, 6 (2): 82-86 [Weng XY, Mi ZH. The human intestinal microflora and disease [J]. Public Health Clin Med, 2010, 6 (2): 82-86]
8 Quigley EMM. The enteric microbiota in the pathogenesis and management of constipation [J]. Best Practice Res Clin Gastroenterol, 2011, 25 (1): 119-126
9 赵兴兵, 吴维佳, 李丹丹, 李湘玉, 张华玲, 谭周进, 蔡光先. 小鼠脾虚便秘造模对肠道微生物及酶活性的影响[J]. 中国微生态学杂志, 2013, 25 (9): 993-996 [Zhao XB, Wu WJ, Li DD, Li XY, Zhang HL, Tan ZJ, Cai GX. The effect of modeling spleen-deficiency constipation on the intestinal microbiota and enzyme activities in mice [J]. Chin J Microecol, 2013, 25 (9): 993-996]
10 周健波. 利用扩增核糖体基因限制性分析法研究竹鼠盲肠细菌多样性[D]. 南宁: 广西大学, 2012 [Zhou JB. Research rhizomyidae cecum bacterial diversity by ARDRA [D]. Nanning: Guangxi University, 2012]
11 Zheng F, Fu MY, Zhao ZX, Chen MC. Community diversity of associated bacteria of Radopholus similis [J]. Plant Dis Pests, 2012, 3 (2): 33-37
12 Gao F, Yi ZZ, Gong J, Al-Rasheid Khaled AS, Song WB. Molecular phylogeny and species separation of fivemorphologically similar Holosticha-complex ciliates(Protozoa, Ciliophora) using ARDRA riboprinting and multigene sequence data [J]. Chin J Oceanol Limnol, 2010, 28 (3): 542-548
13 邹颖, 郑学宝, 戴世学, 叶秋丽, 吴丹. 小鼠脾虚便秘模型的建立[J]. 北京中医药, 2009, 28 (1): 60-62 [Zou Y, Zheng XB, Dai SX, Ye QL, Wu D. Established mouse model of spleen constipation [J]. Beijing J Trad Chin Med, 2009, 28 (1): 60- 62]
14 Eckburg PB, Bik EM, Bernstein CN, Bernstein CN, Purdom E, Dethlefsen L, Sargen M, Gill SR, Nelson KE, Relman DA. Diversity of the human intestinal microbial flora [J]. Science, 2005, 308: 1635-1638.
15 吴海. 超微七味白术散对肠道微生物分子多样性的影响[D]. 长沙: 湖南中医药大学, 2012 [Wu H. Effects of Ultra-micro Qiweibaizhusan on molecular diversity of intestinal microbes [D]. Changsha: Hunan University TCM, 2012]
16 中华人民共和国药典委员会. 中华人民共和国药典(一部)[S]. 北京: 中国医药科技出版社, 2010: 266 [Pharmacopoeia Committee of People’s Republic of China. People’s Republic of China Pharmacopoeia. Beijing: Chinese Medical Science and Technology Press, 2010: 266]
17 吴海, 周赛男, 郭纯, 谭周进, 蔡光先, 曾奥, 张华玲. 一种基于PCR分析多样性的小鼠肠道微生物宏基因组提取方法[J]. 中国微生态学杂志, 2012, 24 (7): 648 -651 [Wu H, Zhou SN, Guo C, Tan ZJ, Cai GX, Zeng A, Zhang HL. A metagenome DNA extracting method of intestinal flora in mice for molecular diversity analysis based on PCR technology [J]. Chin J Microecol, 2012, 24 (7): 648 -651]
18 魏小勇. 石斛属植物生物碱研究进展[J]. 中国药事, 2005, 19 (7): 445-447 [Wei XY. Review on the research progress of alkaloid in Dendrobium plants [J]. Chin Pharm Affairs, 2005, 19 (7): 445-447]
19 李玲, 邓晓兰, 赵兴兵, 曾勇, 欧阳冬生. 铁皮石斛化学成分及药理作用研究进展[J]. 肿瘤药学, 2011, 1 (2): 90-93 [Li L, Deng XL, Zhao XB, Zeng Y, Ouyang DS. Advances in studies on chemical constituents in Dendrobium candidum and their pharmacological effects [J]. Anti-tum or Pharmacy, 2011, 1 (2): 90-93]
20 李蕾, 丁长春, 李付惠. 两种石斛多糖的抑菌作用研究[J]. 安徽农业科学, 2011, 39 (10): 5753-5754 [Li L, Ding CC, Li FH. Study on the antibacterial effects of two Dendrobium polysaccharides [J]. J Anhui Agric Sci, 2011, 39 (10): 5753-5754]
21 张周英, 杨成密, 蓝忠, 黄群, 梁林源. 石斛多糖的抗菌作用研究[J]. 中国医药指南, 2012, 10 (33): 439-440 [Zhang ZY, Yang CM, Lan Z, Huang Q, Liang LY. Effect of Dendrobium polysaccharides on antimicrobial [J]. Guide China Med, 2012, 10 (33): 439-440]
22 查学强, 王军辉, 潘利华, 罗建平, 吕明明. 石斛多糖体外抗氧化活性的研究[J]. 食品科学, 2007, 10 (28): 92-93 [Zha XQ, Wang JH, Pan LH, Luo JP, Lu MM. Study on antioxidant activity of polysaccharides from Dendrobium species [J]. Food Sci, 2007, 10 (28): 92-93]
23 赵兴兵, 谢雪姣, 吴维佳, 张华玲, 李丹丹, 佘颜, 谭周进, 蔡光先. 超微铁皮石斛对脾虚便秘小鼠肠道乳酸杆菌多样性的影响[J]. 微生物学报, 2014, 41 (9): 1764-1770 [Zhao XB, Xie XJ, Wu WJ, Zhang HL, Li DD, She Y, Tan ZJ, Cai GX. Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal Lactobacillus in mice with spleen-deficiency constipation [J]. Microbiol China, 2014, 41 (9): 1764-1770]

Memo

Memo:
-
Last Update: 2015-02-15