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Molecular characterization and phylogenetic analysis of endophytic nitrogen-fixing bacteria in Oryza australiensis(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 04
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Molecular characterization and phylogenetic analysis of endophytic nitrogen-fixing bacteria in Oryza australiensis
YUAN Hongjuan YAN Hui YANG Fang PENG Guixiang TAN Zhiyuan
1College of Agriculture, South China Agricultural University, Guangzhou 510642, China2Department of Life Science, Yuncheng University, Yuncheng 044000, China 3College of Resources Environment, South China Agricultural University, Guangzhou 510642, China
Oryza australiensis genetic diversity endophytic nitrogen-fixing bacteria DNA fingerprinting phylogenetic analysis
Q939.11+3.03 : Q948.12+2.3

This research aimed to investigate the diversity of endophytic nitrogen-fixing bacteria in Oryza australiensis. Forty-five strains of endophytic diazotrophs were isolated from wild rice O. australiensis by using two selective nitrogen-free media, and analyzed for phylogenetic and nitrogen-fixing characteristics. The obtained endophytic nitrogen-fixing bacteria were divided into 8 clusters by using SDS-PAGE of whole-cell protein and IS-PCR fingerprinting. The strains were found to be able to maintain higher nitrogenase activity under acid (pH 5.0) or alkaline (pH 9.0) conditions. The highest nitrogen fixation activity occurred in 2% NaCl concentration. None of the strains showed nitrogenase activity at NH4+ of 7.5 mmol/L. Among the 8 clusters, Cluster Ⅰ had 10 strains, followed by 4 strains of Cluster Ⅱ, 3 of Clusters Ⅲ, Ⅳ and Ⅴ, and 2 of Clusters Ⅵ, Ⅶ and Ⅷ. The homology was 97% between Cluster Ⅰ and Burkholderia cepacia ATCC 25416T, 99% between Cluster Ⅴ and Herbaspirillum seropedicae DX35 T(KF357956.1), 100% between Cluster Ⅱ and Klebsiella variicola CPB S30T(KF770986.1), 99% between Cluster Ⅲ and Pantoea agglomerans PB17T(EU360112.1), and 99% between Cluster Ⅶ and Sinorhizobium sp. T2T(FJ687994.1). The results suggested that endophytic nitrogen-fixing bacteria of O. australiensis have genetic diversity, with different strains being able to use different carbon sources.


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Last Update: 2014-08-26