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Breeding of high salt-tolerant Saccharomyces cerevisiae strains based on continuous ethanol fermentation(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 03
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Breeding of high salt-tolerant Saccharomyces cerevisiae strains based on continuous ethanol fermentation
ZHOU Li TANG Yueqin SUN Zhaoyong KIDA Kenji
Architecture and Environment College, Sichuan University, Chengdu 610065, China
Saccharomyces cerevisiae salt tolerance continuous ethanol fermentation tetrad analysis fuel ethanol trehalose

Waste biomass resources such as molasses and kitchen waste are important raw materials for the production of fuel ethanol. Breeding of salt-tolerant Saccharomyces cerevisiae strain is of great importance for reducing the production cost of fuel ethanol from these wastes with high salt content. Strain KF-7, an industrial strain with excellent ethanol fermentation ability, was used as original strain in this study. Strains with stable salt tolerance were bred through continuous fermentation followed by sporulation, spore germination and mating. The intracellular trehalose contents of mutants and parent strain KF-7 were compared to reveal the relationship between the salt tolerance and the ability of trehalose accumulation. By continuous ethanol fermentation under salt stress condition, mutant strain KF-7(4) with improved salt tolerance was isolated. Salt tolerant strains KF-7(4)-3 and KF-7-D1 were obtained, respectively, through spore germination and mating of salt tolerant haploids of mutant strain KF-7(4). The improved salt tolerance of these three strains remained stable after 50-time subcultivation. Under the condition of 9% KCl, the ethanol fermentation ability of the three strains was higher than that of the original strain KF-7. Compared to strain KF-7, they produced approximately 21% higher ethanol at 36 h when 15% YPD medium was used. The ability of trehalose accumulation of strains KF-7(4) and KF-7(4)-3 was significantly higher than that of the original strain KF-7 even without salt stress. The results showed stable and higher salt tolerance of S. cerevisiae mutant strains than their parent strain KF-7, which is probably due to the improved ability of trehalose accumulation. Evolution engineering based on continuous fermentation showed high potential for breeding S. cerevisiae mutants with expected characters.


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Last Update: 2014-07-01