|Table of Contents|

Isolation and identification of Aspergillus niger Y9 and its role in bioleaching of metal contaminants from soils(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2014 03
Page:
420-425
Research Field:
Articles
Publishing date:

Info

Title:
Isolation and identification of Aspergillus niger Y9 and its role in bioleaching of metal contaminants from soils
Author(s):
CUI Yuqi;  FANG Di;  BI Wenlong;  CUI Chunhong;  ZHOU Lixiang
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Aspergillus niger;  soils;  heavy metals;  organic acids;  bioleaching
CLC:
X172 : X53
PACS:
DOI:
10.3724/SP.J.1145.2014.11019
DocumentCode:

Abstract:
Fungus is reported to have the capability of producing organic acids that can serve as leaching agents for removing heavy metals from the environment. In this study, an acid-producing heterotrophic fungus named Y9 was isolated from soil and identified as Apergillus niger through morphological and molecular identification. Furthermore, its growth and acid production characteristics, as well as its application in leaching heavy metals from contaminated soil by the one-step and two-step bioleaching processes were investigated and evaluated in shake flask culture. The results showed that A. niger Y9 had not only a wide range of optimal pH value from 3.0 to 8.0, but also a strong tolerance to Zn2+, Cd2+ and Pb2+ and the 80% inhibitory concentration was 250 mg/L, 130 mg/L, 2 700 mg/L, respectively. After incubating for 20 days, this fungus excreted 54.8 mmol/L citric acid, 20.1 mmol/L gluconic acid, 15.9 mmol/L oxalic acid and 6.3 mmol/L malic acid. As a result, pH value of the pure culture system declined to 2.1. Besides, in the bioleaching experiment, the two-step process exhibited a relatively higher leaching efficiency of heavy metals with a maximum solubilization of 75% Zn, 100% Cd and 43% Pb, as compared to the one-step bioleaching process with removal efficiencies of 64% Zn, 86% Cd, and 19% Pb. It implied that Aspergillus niger Y9 has a good resistance of heavy metals and could be used to remove heavy metals from contaminated soils.

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Last Update: 2014-07-01