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Effect of Methanosaeta on anaerobic granular sludge formation(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 02
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Effect of Methanosaeta on anaerobic granular sludge formation
GONG Shufen ZHENG Mingyue MA Hailing WANG Kaijun LI Lingyan DONG Xiuzhu
1State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China 2School of Water Resource and Environment, China University of Geosciences, Beijing 100083, China 3 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
EGSB organic load granular sludge Methanosaeta
X172 : X703.5

In order to understand the effect of filamentous cells of Methanosaeta harundinacea 6Ac on granular sludge formation under the medium temperature in the EGSB reactor, as well as the properties of the granular sludge formed and operation of the reactor, we cultivated granular sludge with two laboratory scale reactors under the constant temperature condition of 35 ± 1 °C. One reactor (R2) was inoculated anaerobic flocculent sludge with a bristle methane bacteria species; the other reactor (R1) was the control only inoculated with the anaerobic flocculent sludge. The results showed that when the organic load gradually increased to 11.1 kg m-3 d-1, the COD removal rate of R2 reached 85.9%, higher than that of R1 (75.0%). Granular sludge was founded in both reactors after 46 days, with 62.3% sludge particles greater than 0.1 mm in R2. During 28-46 days, the percentage of sludge particles 0.1-0.3 mm increased from 10.0% to 49.8% in R2, but only from 10% to 33.3% in the control group R1, where flocculent sludge was mainly detected. This research showed that adding Methanosaeta could promote formation of granular sludge, enhance its proliferation and stability, improve precipitation of granular sludge and removal of organic matter, and accelerate start-up of EGSB.


1 左剑恶, 王妍春, 陈浩, 左宜, 申强. EGSB反应器的启动运行研究[J]. 给水排水, 2001, 27 (3): 26-30 [Zuo JW, Wang YC, Chen H, Zuo Y, Shen Q. The study of EGSB reactor start-up operation [J]. China Water Wastewater, 2001, 27 (3): 26-30]
2 Randall AW, Richard RD. Enhancement of granulation and start-up in the anaerobic sequencing batch reactor. Water Environ Res, 1996, 68: 883-892
3 Imai T, Ukita M, Liu J, Sekine M, Nakanishi H, Fukagawa M. Advanced start-up of UASB reactors by addition of water absorbing polymer [J].Water Sci Technol, 1997, 36 (6): 399-406
4 曹玉成, 张妙仙, 单胜道. 两次投加竹炭对UASB反应器污泥颗粒化的促进作用[J]. 农业工程学报, 2010, 26 (5): 246-250 [Cao YC, Zhang MX, Dan SD. Effect of two-added powdered bamboo-charcoal on sludge granulation of UASB reactor [J]. Trans CSAE, 2010, 26 (5): 246-250]
5 韩剑宏, 倪文, 江翰. 投加硬硅钙纤维粒对UASB低温运行的影响[J]. 中国给水排水, 2003, 19 (10): 4-17 [Han JH, Ni W, Jiang H. Influence of addition of xonotlite fibrous particle on UASB operation at low temperature [J]. China Water Wastewater, 2003, 19 (10): 4-17]
6 李保石, 佘宗莲, 樊玉清, 于晓静. 投加阳离子聚合物加速UASB反应器中颗粒污泥形成[J]. 环境工程学报, 2009, 7 (3): 1322-1325 [Li BS, She ZL, Fan YQ, Yu XJ. Accelerated granulation by cationic polymer in UASB reactor [J]. Chin J Environ Eng, 2009, 7 (3): 1322-1325
7 王劲松, 胡勇有. 微生物絮凝剂促进厌氧污泥颗粒化及其机制的研究[J]. 环境科学学报, 2005, 25 (3): 361-366 [Wang JS, Hu YY, Mechanism of anaerobic granulation enhancement with bio-flocculant [J]. Acta Sci Circumst, 2005, 25 (3): 361-366]
8 Yu HQ, Tay JH, Fang Herbert HP. Effect of added powdered and granular activated carbons on start-up performance of UASB reactors [J]. Environ Techno1, 1999, 20: 1095-1101
9 Ma K, Liu XL, Dong XZ. Methanosaeta harundinacea sp. nov, a novel acetate-scavenging methanogen isolated from a UASB reactor. Intern J Syst Evol Microbiol, 2006, 56: 127-131
10 MacLeod FA, Guiot SR, Costerton JW. Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Appl Environ Microbiol, 1990, 56 (6): 1598-1607
11 国家环境保护局. 水和废水监测分析方法[M]. 4版. 北京: 中国环境科学出版社, 2002 [The State Environmental Protection Agency. Water and Wastewater Monitoring Analysis Method [M]. 4th ed. Beijing: China Environmental Science Press, 2002
12 贺延龄. 废水的厌氧生物处理[M]. 北京: 中国轻工业出版社, 1998. 509-542 [He YL. Anaerobic Biological Treatment of Wastewater [M]. Beijing: China Light Industry Press, 1998. 509-542
13 Letting G, van Velsen AFM, Hobma SW, de Zeeuw W, Klapwijk A. Use of upflow sludge blanket (USB) reactor concept for biological wasterwater treatmengt, especially for anaerobic treatment [J]. Biotechnol Bioeng, 1980, 22 (4): 699-734
14 Hulshoff Pol L. The phenomena of granulation of anaerobic sludge [D]. The Netherlands: Wangeningen University, 1989
15 Schmidt JE, Ahring BK. Granular sludge formation in upflow anaerobic sludge blanket (UASB) reactors [J]. Biotechnol Bioeng, 1996, 49 (3): 229-232
16 EIMamouni R, Leduc R, Guiot SR. Influence of synthetic and natural polymers on the anaerobic granulation process. Water Sci Technol, 1998, 38 (8-9): 341-347


Last Update: 2014-05-04