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Antimicrobial and antioxidant activities of extracts from walnut green husks(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 01
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Antimicrobial and antioxidant activities of extracts from walnut green husks
ZHANG Weixing HE Kaize PU Qiang
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
walnut green husk extracts antimicrobial activity antioxidant activity
Q949.9 : Q949.720.6

This research aimed to evaluate the antimicrobial and antioxidant activities of walnut green husks, and to explore possible ways to efficiently utilize the bioresource. After walnut green husks were extracted by different reagents, the antimicrobial capacity of the extracts was measured by Oxford Cup method; the antioxidant capacity was assessed by total phenolic content, reducing power assay and scavenging effects on DPPH (2,2-diphe-nyl-1-picrylhydrazyl) radicals. All extracts showed different antimicrobial activities against Gram negative (Escherichia coli, Pseudomonas aeruginosa), Gram positive (Staphyloccocus aureus, Bacillus subtilis) bacteria, and fungi (Canidia albicans, Penicillium italicum, Peniicilbum digitatum, Phytophthora capsici). The walnut green husks extracted with ethyl acetate and chloroform showed the highest activities against bacteria tested, with minimum inhibitory concentrations (MIC) against the four bacteria about 6.25/6.25, 6.25/1.56, 3.13/3.13, 6.25/3.13 mg/mL, respectively. On the other hand, antifungal substances existed in petroleum ether extracts, with MIC against Penicillium italicum, Peniicilbum digitatum and Canidia albicans about 3.13, 6.25 and 6.25 mg/mL respectively. The total phenol contents in ethyl acetate and chloroform extracts were about 193.52 and 174.64 mg GAEs/g respectively their EC50 of scavenging DPPH radical activity about 14.05 and 26.11 μg/mL, EC50 of reducing power about 34.70 and 44.60 μg/mL. The antioxidant activities of the extracts with other solvents were much lower than those extracted with ethyl acetate and chloroform. Our research showed that walnut green husk contains antibacterial and antioxidant material in its ethyl acetate and chloroform extracts, and antifungal material in its petroleum ether extracts.


1 高海生, 朱凤妹, 李润丰. 我国核桃加工产业的生产现状与发展趋势[J]. 经济林研究, 2008, 26 (3): 119-126 [Gao HS, Zhu FM, Li RF. Production status and development trend of walnut processing industry in China [J]. Nonwood ForRes, 2008, 26 (3): 119-126]
2 Stampar F, Solar A, Hudina M, Veberic R, Colaric M. Traditional walnut liqueur – cocktail of phenolics [J]. Food Chem, 2006, 95 (4): 627-631
3 Yang JA, Qiu KQ. Preparation of activated carbons from walnut shells via vacuum chemical activation and their application for methylene blue removal [J]. Chem Eng J, 2010, 165 (1): 209-217
4 Pereira JA, Oliveira I, Sousa A, Valentao P, Andrade PB, Ferreira I, Ferreres F, Bento A, Seabra R, Estevinho L. Walnut (Juglans regia L.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars [J]. Food Chem Toxicol, 2007, 45 (11): 2287-2295
5 Lee KS, Li G, Kim SH, Lee CS, Woo MH, Lee SH, Jhang YD, Son JK. Cytotoxic diarylheptanoids from the roots of Juglans mandshurica [J]. J Nat Prod, 2002, 65 (11): 1707-1708
6 潘富赟, 张培正. 核桃青皮的综合应用及开发前景. 中国食物与营养, 2010, 135 (12): 21-24 [Pan FB, Zhang PZ. Comprehensive application and development prospect of walnut green husk. Food Nutr China, 2010, 135 (12): 21-24]
7 Berker KI, Guclu K, Tor I, Demirata B, Apak R. Total antioxidant capacity assay using optimized ferricyanide/prussian blue method [J]. Food Anal Methods, 2010, 3 (3): 154-168
8 Brandwilliams W, Cuvelier ME, Berset C. Use of a free-radical method to evaluate antioxidant activity [J]. Food Sci Technol-Lebensmittel-Wissenschaft Technol, 1995, 28 (1): 25-30
9 曲建博, 娄红祥, 范培红. TLC生物自显影技术在药物筛选中的应用. 中草药, 2005, 36 (1): 132-137 [Qu JB, Lou HX, Fan PH. Application of TLC-bioautography to drug screening. Chin Trad Herbal Drugs, 2005, 36 (1): 132-137]
10 Horvath G, Jambor N, Vegh A, Boszormenyi A, Lemberkovics E, Hethelyi E, Kovacs K, Kocsis B: Antimicrobial activity of essential oils: the possibilities of TLC-bioautography [J]. Flavour Fragrance J, 2010, 25 (3): 178-182
11 Chen Y, Xie MY, Nie SP, Li C, Wang YX. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum [J]. Food Chem, 2008, 107 (1): 231-241
12 Odabasoglu F, Aslan A, Cakir A, Suleyman H, Karagoz Y, Bayir Y, Halici M. Antioxidant activity, reducing power and total phenolic content of some lichen species [J]. Fitoterapia, 2005, 76 (2): 216-219
13 Pereira JA, Oliveira I, Sousa A, Valent?o P, Andrade PB, Ferreira ICFR, Ferreres F, Bento A, Seabra R, Estevinho L. Walnut (Juglans regia L.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars [J]. Food Chem Toxicol, 2007, 45 (11): 2287-2295
14 Rauha JP, Remes S, Heinonen M, Hopia A, Kahkonen M, Kujala T, Pihlaja K, Vuorela H, Vuorela P. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds [J]. Intern J Food Microbiol, 2000, 56 (1): 3-12
15 Heinonen M. Antioxidant activity and antimicrobial effect of berry phenolics - a Finnish perspective [J]. Mol Nutr Food Res, 2007, 51 (6): 684-691
16 宋立江, 狄莹, 石碧. 植物多酚研究与利用的意义及发展趋势. 化学进展, 2000, 12 (2): 161-170 [Song LJ, Di Y, Shi B. The significance and development trend in research of plant polyphenols. Progr Chem, 2000, 12 (2):161-170]
17 延玺, 刘会青, 邹永青, 任占华. 黄酮类化合物生理活性及合成研究进展. 有机化学, 2008, 238 (9): 1534-1544 [Yan X, Liu HQ, Zou YQ, Ren ZH. Physiological activities and research advance in synthesis of flavonoids. Chin J Org Chem, 2008, 28 (9): 1534-1544]
18 Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids [J]. Intern J Antimicrob Agents, 2005, 26 (5): 343-356
19 Yi ZB, Yu Y, Liang YZ, Zeng B. In vitro antioxidant and antimicrobial activities of the extract of Pericarpium Citri Reticulatae of a new Citrus cultivar and its main flavonoids [J]. Lwt-Food Sci Technol, 2008, 41 (4): 597-603
20 Alkhawajah AM. Studies on the antimicrobial activity of Juglans regia [J]. Am J Chin Med, 1997, 25 (2): 175-180
21 Ohta A, Sivalingam PM, Lin S, Ikekawa N, Yaginuma N, Inada Y. Isolation of naphthazarin from walnut ‘Onigurumi’, and its inhibitory action on oxidative phosphorylation in mitochondria [J]. Toxicon, 1973, 11 (3): 235-241


Last Update: 2014-03-06