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Sequence analysis and blue light-influenced expression of histidine kinase gene (CmHK) from fungus Cordyceps militaris(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2014 01
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Sequence analysis and blue light-influenced expression of histidine kinase gene (CmHK) from fungus Cordyceps militaris
GAO Ya JIN Huayan FU Mingjia SHEN Junliang LI Qin
College of Life Sciences, Jiangxi Normal University, Nanchang 330027, China
Cordyceps militaris histidine kinase blue light real-time PCR
Q939.5 : Q78

Blue light is a very important environmental factor causing physiological and biochemical changes in organisms. Histidine kinase is an important component in the two-component signal transduction system in many organisms. To determine the expression of histidine kinase gene (CmHK) influenced by blue light in fungus Cordyceps militaris, the CmHK ORF sequence of C. militaris was cloned and analyzed. The total RNA was extracted from fungus C. militaris, and the primers designed to obtain a histidine kinase gene (CmHK) ORF sequences of C. militaris by RT-PCR. The real-time PCR method was used to the CmHK gene expression analysis in the C. militaris mycelia with different blue light irradiation duration. The result confirmed that the CmHK gene ORF sequence was obtained. The bioinformatics analysis for the translation products of CmHK gene showed 4 HAMP functional domains, 1 HisKA functional domain, 1 HATPase domain and 1 REC functional domain in CmHK sequence. Putting C. militaris mycelium into continuous 24h blue light irradiation, and using real-time PCR to analyze the expression of CmHK gene in C. militaris during the blue light irradiation, the results showed that the CmHK gene expression had an obvious decrease in the beginning of blue light irradiation, soon afterwards sustained a concussion rising and reached a peak at 16 h, then decreased rapidly. These results suggested that the CmHK in C. militaris has the typical characteristics of histidine kinase, and that direct exposure to blue light can change the expression level of CmHK gene in C. militaris.


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Last Update: 2014-03-06