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Improved Drought Tolerance of Transgenic Arabidopsis thaliana by Inducible Expression of XERICO Gene(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2013 06
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Improved Drought Tolerance of Transgenic Arabidopsis thaliana by Inducible Expression of XERICO Gene
HOU Pei FENG Yuanyuan YU Guirong REN Pengfei ZENG De’er
(1Key Laboratory of Ministry of Education for Bio-resources and Eco-environment, College of Life Sciences, and State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China) (2Department of Science Education, Chengdu Normal University, Chengdu, Sichuan 611130)
Arabidopsis thaliana SARK promoter XERICO gene ABA drought tolerance

To develop drought-resistant plants or crops, we constructed the PSARK::XERICO plant expression vector and transformed it into the model plant Arabidopsis thaliana (Col-0 ecotype) to analyze the physiological phenotypes. In this study, SARK promoter was isolated from recombinant plasmid of pSARK-IPT; full length of XERICO gene was amplified from the cDNA of Arabidopsis seedlings by the RT-PCR technique; the SARK promoter and the XERICO gene replaced CaMV 35S promoter and GUS gene respectively to obtain the recombinant plasmid, designated as pBI121-pSARK-XERICO. The constructed expression vector was transformed into the wild-type Arabidopsis through the floral dip method. The homozygous lines were obtained through selection of antibiotic resistance and analysis of genetic segregation. The obtained homozygous seedlings were assayed by PCR and evaluated through abscisic acid (ABA) sensitivity analysis during seed germination, and analyzed through the results of phenotypic observation after salinity and mannitol stress treatment during early seedling growth. In addition, the analysis of drought tolerance was conducted between the transgenic PSARK::XERICO and wild-type Arabidopsis. Compared with the wild-type and xerico mutant plants, the transgenic PSARK::XERICO plants were more sensitive to 1.0 μmol/L ABA, 100 mmol/L NaCl and 200 mmol/L mannitol. The expression of water deficit-inducible XERICO gene in Arabidopsis enhanced the tolerance to drought stress. Therefore, this study indicated that PSARK::XERICO has the potential for breeding drought-tolerant plants or crops.


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