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Expression of Endoglucanase Gene and β-Glucosidase Genes in Bacillus subtilis(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2013 06
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Expression of Endoglucanase Gene and β-Glucosidase Genes in Bacillus subtilis
WANG Yuan GAO Qiuqiang XIN Xiujuan BAO Jie
(State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China)
Bacillus subtilis β-glucosidase endoglucanase extracellular secretion coexpression
Q786 : Q814

In order to archive the secretory coexpression of cellulase genes, this study used the pP43JM2 shuttle vector carrying NprB signal peptide of the Bacillus subtilis neutral protease as expression vector to express in B. subtilis WB800 the celA gene from Clostridium thermocellum DSM 1237 encoding endoglucanase and bglA and bglB genes from B. polymyxa encoding β-glucosidases. The results showed that the endoglucanase encoded by celA and the β-glucosidases encoded by bglB were secreted into the culture broth successfully; the two cellulase genes could be coexpressed and secreted; the extracellular enzyme solution reacted with PASC and cellobiose, producing 674 and 24 mg/L glucose, respectively; on the contrary, the β-glucosidases encoded by bglA did not react with PASC, only with cellobiose, its intracellular enzyme solution producing 938.7 mg/L glucose. The current work suggested a possible method for multiple cellulase secretion in B. subtilis and may provide a way of economical construction of bioprocessing strains for bulk chemical production.


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