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RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription Factor OsMYB14(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2013 06
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RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription Factor OsMYB14
MIAO Yanwen LI Rong XIONG Fangjie ZENG Zhengming NIU Xiangli LIU Yongsheng
(1School of Life Sciences, Chongqing University, Chongqing 400000, China) (2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
Oryza sativa L. MYB transcription factor RNA interference abiotic stress tiller
Q943.2 : S511.034

MYB (myeloblastosis) transcription factors play vital roles in the regulation of plant development and defense responses. This study isolated a rice R2R3-MYB, OsMYB14, the expression of which was down-regulated by abiotic stress, such as salt, drought and UV-B (ultraviolet-B, 280-320 nm). RNA interference (RNAi) vector of OsMYB14 was constructed and introduced into rice cultivar Nipponbare by Agrobacterium-mediated transformation. Real-time PCR revealed a significant decrease of OsMYB14 expression level in transgenic lines. Compared with wild-type plants, the transgenic plants exhibited more spikes and tillers. No difference in growth and development between the transgenic lines and wild-type plants was observed under normal conditions, but the transgenic seedlings were more tolerant to salt, drought and UV-B, yet less sensitive to abscisic acid (ABA) than wild-type plants. These results suggested that OsMYB14 encodes an ABA-dependent, growth-regulatory and stress-responsive MYB transcription factor that plays an important role in tolerance of rice to salt, drought and UV-B.


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Last Update: 2014-01-03