|Table of Contents|

RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription Factor OsMYB14(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2013 06
Page:
960-968
Research Field:
Articles
Publishing date:

Info

Title:
RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription Factor OsMYB14
Author(s):
MIAO Yanwen LI Rong XIONG Fangjie ZENG Zhengming NIU Xiangli LIU Yongsheng
(1School of Life Sciences, Chongqing University, Chongqing 400000, China) (2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
Keywords:
Oryza sativa L. MYB transcription factor RNA interference abiotic stress tiller
CLC:
Q943.2 : S511.034
PACS:
DOI:
10.3724/SP.J.1145.2013.00960
DocumentCode:

Abstract:
MYB (myeloblastosis) transcription factors play vital roles in the regulation of plant development and defense responses. This study isolated a rice R2R3-MYB, OsMYB14, the expression of which was down-regulated by abiotic stress, such as salt, drought and UV-B (ultraviolet-B, 280-320 nm). RNA interference (RNAi) vector of OsMYB14 was constructed and introduced into rice cultivar Nipponbare by Agrobacterium-mediated transformation. Real-time PCR revealed a significant decrease of OsMYB14 expression level in transgenic lines. Compared with wild-type plants, the transgenic plants exhibited more spikes and tillers. No difference in growth and development between the transgenic lines and wild-type plants was observed under normal conditions, but the transgenic seedlings were more tolerant to salt, drought and UV-B, yet less sensitive to abscisic acid (ABA) than wild-type plants. These results suggested that OsMYB14 encodes an ABA-dependent, growth-regulatory and stress-responsive MYB transcription factor that plays an important role in tolerance of rice to salt, drought and UV-B.

References

1 Munns R, Tester M. Mechanisms of salinity tolerance [J]. Annu Rev Plant Biol, 2008, 59: 651-681 2 Zhu JK. Salt and drought stress signal transduction in plants [J]. Annu Rev Plant Biol, 2002, 53: 247-273 3 Xin Z, Browse J. Eskimo1 mutants of Arabidopsis are constitutively freezing-tolerant [J]. Proc Natl Acad Sci USA, 1998, 95 (13): 7799-7804 4 Mitsuda N, Ohme-Takagi M. Functional analysis of transcription factors in Arabidopsis [J]. Plant Cell Physiol, 2009, 50(7): 1232-1248 5 Takasaki H, Maruyama K, Kidokoro S, Ito Y, Fujita Y, Shinozaki K, Yamaguchi-Shinozaki K, Nakashima K, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice [J]. Mol Genet Genomics, 2010, 284 (3): 173-183 6 Xiang Y, Tang N, Du H, Ye H, Xiong L. Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice [J]. Plant Physiol, 2008, 148 (4): 1938-1952 7 Yang A, Dai X, Zhang WH. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice [J]. J Exp Bot, 2012, 63 (7): 2541-2556 8 Klempnauer KH, Gonda TJ, Bishop JM. Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: the architecture of a transduced oncogene [J]. Cell, 1982. 31 (2 Pt 1): 453-463 9 Paz-Ares J, Ghosal D, Wienand U, Peterson PA, Saedler H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators [J]. EMBO J, 1987, 6 (12): 3553-3558 10 Ogata K, Hojo H, Aimoto S, Nakai T, Nakamura H, Sarai A, Ishii S, Nishimura Y. Solution structure of a DNA-binding unit of Myb: a helix-turn-helix-related motif with conserved tryptophans forming a hydrophobic core [J]. Proc Natl Acad Sci USA, 1992, 89 (14): 6428-6432 11 Stracke R, Werber M, Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana [J]. Curr Opin Plant Biol, 2001, 4 (5): 447-456 12 Chen Y, Yang X, He K, Liu M, Li J, Gao Z, Lin Z, Zhang Y, Wang X, Qiu X, Shen Y, Zhang L, Deng X, Luo J, Deng XW, Chen Z, Gu H, Qu L The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family [J]. Plant Mol Biol, 2006, 60 (1): 107-124 13 Mahjoub A, Hernould M, Joubes J, Decendit A, Mars M, Barrieu F, Hamdi S, Delrot S. Overexpression of a grapevine R2R3-MYB factor in tomato affects vegetative development, flower morphology and flavonoid and terpenoid metabolism [J]. Plant Physiol Biochem, 2009, 47 (7): 551-561 14 Stracke R, Ishihara H, Huep G, Barsch A, Mehrtens F, Niehaus K, Weisshaar B. Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling [J]. Plant J, 2007, 50 (4): 660-677 15 Yamagishi M, Shimoyamada Y, Nakatsuka T, Masuda K. Two R2R3-MYB genes, homologs of Petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of asiatic hybrid lily [J]. Plant Cell Physiol, 2010, 51 (3): 463-474 16 Higginson T, Li SF, Parish RW. AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana [J]. Plant J, 2003, 35 (2): 177-192 17 Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L. TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana [J]. Plant J, 2004, 39 (3): 366-380 18 Shin B, Choi G, Yi H, Yang S, Cho I, Kim J, Lee S, Paek NC, Kim JH, Song PS, Choi G. AtMYB21, a gene encoding a flower-specific transcription factor, is regulated by COP1 [J]. Plant J, 2002, 30 (1): 23-32 19 Penfield S, Meissner RC, Shoue DA, Carpita NC, Bevan MW. MYB61 is required for mucilage deposition and extrusion in the Arabidopsis seed coat [J]. Plant Cell, 2001, 13 (12): 2777-2791 20 Araki S, Ito M, Soyano T, Nishihama R, Machida Y. Mitotic cyclins stimulate the activity of c-Myb-like factors for transactivation of G2/M phase-specific genes in tobacco [J]. J Biol Chem, 2004, 279 (31): 32979-32988 21 Shan H, Chen S, Jiang J, Chen F, Chen Y, Gu C, Li P, Song A, Zhu X, Gao H, Zhou G, Li T, Yang X. Heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana [J]. Mol Biotechnol, 2012, 51 (2): 160-173 22 El-Kereamy A, Bi YM, Ranathunge K, Beatty PH, Good AG, Rothstein SJ. The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism [J]. PLoS One, 2012, 7 (12): e52030 23 Pasquali G, Biricolti S, Locatelli F, Baldoni E, Mattana M. Osmyb4 expression improves adaptive responses to drought and cold stress in transgenic apples [J]. Plant Cell Rep, 2008, 27 (10): 1677-1686 24 Denekamp M, Smeekens SC. Integration of wounding and osmotic stress signals determines the expression of the AtMYB102 transcription factor gene [J]. Plant Physiol, 2003, 132 (3): 1415-1423 25 Newman LJ, Perazza DE, Juda L, Campbell MM. Involvement of the R2R3-MYB, AtMYB61, in the ectopic lignification and dark-photomorphogenic components of the det3 mutant phenotype [J]. Plant J, 2004, 37 (2): 239-250 26 Gocal GF, Sheldon CC, Gubler F, Moritz T, Bagnall DJ, MacMillan CP, Li SF, Parish RW, Dennis ES, Weigel D, King RW. GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis [J]. Plant Physiol, 2001, 127 (4): 1682-1693 27 Park MR, Yun KY, Mohanty B, Herath V, Xu F, Wijaya E, Bajic VB, Yun SJ, De Los Reyes BG. Supra-optimal expression of the cold-regulated OsMyb4 transcription factor in transgenic rice changes the complexity of transcriptional network with major effects on stress tolerance and panicle development [J]. Plant Cell Environ, 2010, 33 (12): 2209-2230 28 Soltesz A, Vagujfalvi A, Rizza F, Kerepesi I, Galiba G, Cattivelli L, Coraggio I, Crosatti C. The rice Osmyb4 gene enhances tolerance to frost and improves germination under unfavourable conditions in transgenic barley plants [J]. J Appl Genet, 2012, 53 (2): 133-143 29 Ma Q, Dai X, Xu Y, Guo J, Liu Y, Chen N, Xiao J, Zhang D, Xu Z, Zhang X, Chong K. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes [J]. Plant Physiol, 2009, 150 (1): 244-256 30 Su CF, Wang YC, Hsieh TH, Lu CA, Tseng TH, Yu SM. A novel MYBS3-dependent pathway confers cold tolerance in rice [J]. Plant Physiol, 2010, 153 (1): 145-158 31 Dai X, Wang Y, Yang A, Zhang WH. OsMYB2P-1, an R2R3 MYB transcription factor, is involved in the regulation of phosphate-starvation responses and root architecture in rice [J]. Plant Physiol, 2012, 159 (1): 169-183 32 Vannini C, Locatelli F, Bracale M, Magnani E, Marsoni M, Osnato M, Mattana M, Baldoni E, Coraggio I. Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants [J]. Plant J, 2004, 37 (1): 115-127 33 Yang S, Sweetman JP, Amirsadeghi S, Barghchi M, Huttly AK, Chung WI, Twell D. Novel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression [J]. Plant Physiol, 2001, 126 (4): 1738-1753 34 Chong J, Baltz R, Schmitt C, Beffa R, Fritig B, Saindrenan P. Downregulation of a pathogen-responsive tobacco UDP-Glc:phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance [J]. Plant Cell, 2002, 14 (5): 1093-1107 35 Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C. Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis [J]. EMBO J, 2000, 19 (22): 6150-6161 36 Szekely G, Abraham E, Cseplo A, Rigo G, Zsigmond L, Csiszar J, Ayaydin F, Strizhov N, Jasik J, Schmelzer E, Koncz C, Szabados L. Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis [J]. Plant J, 2008, 53 (1): 11-28 37 Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T, Vuylsteke M, Leonhardt N, Dellaporta SL, Tonelli C. A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance [J]. Curr Biol, 2005, 15 (13): 1196-1200 38 Guo Y, Gan S. AtMYB2 regulates whole plant senescence by inhibiting cytokinin-mediated branching at late stages of development in Arabidopsis [J]. Plant Physiol, 2011, 156 (3): 1612-1619 39 Schmitz G, Tillmann E, Carriero F, Fiore C, Cellini F, Theres K. The tomato blind gene encodes a MYB transcription factor that controls the formation of lateral meristems [J]. Proc Natl Acad Sci USA, 2002, 99 (2): 1064-1069 40 Muller D, Schmitz G, Theres K. Blind homologous R2R3 Myb genes control the pattern of lateral meristem initiation in Arabidopsis [J]. Plant Cell, 2006, 18 (3): 586-597

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Last Update: 2014-01-03