|Table of Contents|

Overexpression of Pectinase Gene in Endophytic Bacillus Strains and Its Effect on Colonization(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2013 05
Page:
805-810
Research Field:
Articles
Publishing date:

Info

Title:
Overexpression of Pectinase Gene in Endophytic Bacillus Strains and Its Effect on Colonization
Author(s):
FAN Xiaojing YANG Ruixian QIU Sixin HU Fangping
(1College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China) (2Key Laboratory for Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China) (3Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, China) (4Crop research institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China)
Keywords:
endophytic Bacillus pectinase gene Real-time PCR overexpression colonization
CLC:
Q948.12+2.3 : Q78
PACS:
DOI:
10.3724/SP.J.1145.2013.00805
DocumentCode:

Abstract:
In order to investigate the correlation between pectinase gene of endogenous Bacillus and colonization in plants, the full length genes of pectinase from two endophytic Bacillus strains were cloned; the overexpressing vectors of pectinase genes were constructed by inserting the full length genes of pectinase into shuttle vector pGFP4412, then transformed into the endophytic Bacillus strains and overexpressed. Pectinase enzymatic assays showed that the diameters of hydrolyzed circles caused by the wild type strains were only 50% of the overexpressing mutants on LB agar plates. The real-time quantitative PCR results showed that the relative mRNA transcription levels of pectinase gene in mutant bacteria were 82 and 85 times as much as the level in the control strains (BS2-gfp and TB2-gfp), respectively. The colonizing bacteria amounts of pectinase mutants and the control strains in the tissues of cabbage within 28 d indicated a significant difference between mutants and control strains in roots, stems and leaves in the first 1-3 d after inoculation (P < 0.05). The amount of mutant bacteria in roots was notably higher than that of control strains in the 7th day (P < 0.05). But for the other statistic data, no significant difference was detected. The results of continuous isolations during 28 d showed a remarkably higher population density in mutants than the control strains at the beginning, and a gradually decreasing difference between the two. This study presented preliminary evidence that pectinase gene plays a role in endophytic Bacillus bacteria colonization in plant at the initial stage. Fig 7, Tab 2, Ref 23

References

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Last Update: 2013-10-28