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Cloning and Expression Analyses of Sporamin Family Genes in Sweet Potato(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2013 02
Page:
215-223
Research Field:
Articles
Publishing date:

Info

Title:
Cloning and Expression Analyses of Sporamin Family Genes in Sweet Potato
Author(s):
LAI Xianjun GU Yinghong WANG Haiyan ZHANG Yizheng
(Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China)
Keywords:
sweet potato sporamin gene cloning sequence analysis digital gene expression profiling protease inhibitor
CLC:
Q946.1 : Q78
PACS:
DOI:
10.3724/SP.J.1145.2013.00215
DocumentCode:

Abstract:
In order to study the structure and function of sporamin family genes in sweet potato, the transcriptome database of this plant constructed in our lab were analyzed to screen sporamin contigs and appropriate primers designed to amplify sporamin genes, which were then cloned and sequenced. All together 61 sporamin contigs were found in the transcriptome database, including 31 contigs in sporamin A sub-family and 30 contigs in sporamin B sub-family. Six sporamin A genes and 10 sporamin B genes were cloned and sequenced. ORF of all sporamin A genes were 660 bp in length with identity more than 95% among all sporamin A genes. ORF of sporamin B genes had a total length of 651 bp with identity more than 98% among sporamin B genes. Homology between the two sub-families was 84%. The results of digital gene expression profile analysis showed that both sporamin A and B had the highest level of transcription in the initial tuberous roots and harvest tuberous roots, comparatively low transcription in the expanding tuberous roots, and very little in leaves and stems. Analysis of conserved sequence of sporamin genes and homology comparison displayed that the proteins contained a soybean trypsin inhibitor conserved sequence with more than 170 amino acids belonging to the Kunitz-type protease inhibitor superfamily, indicating that the sporamin has the function of protease inhibitors. Fig 9, Tab 3, Ref 33

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Last Update: 2013-05-02