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Construction and Genetic Transformation of the RNA Interference Vector and Functional Analysis of Rice Expansin Gene OsEXPB7(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2013 02
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Construction and Genetic Transformation of the RNA Interference Vector and Functional Analysis of Rice Expansin Gene OsEXPB7
HUANG Longxiang NIU Xiangli XIONG Fangjie ZOU Xiaolong LIU Yongsheng
(1Institute of Agricultural and Life Sciences, Chongqing University, Chongqing 400000, China)
(2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
rice expansin RNA interference Agrobacterium-mediated transformation phytohormone
Q943.2 : S511.01

Expansin, a vital component of plant cell wall, involves in various physiological activities of plants. In the present study, RNA interference (RNAi) vector of rice expansin gene OsEXPB7 was constructed and introduced into rice cultivar Nipponbare by Agrobacterium-mediated transformation. The quantitative PCR analysis revealed the suppression of OsEXPB7 in transgenic lines. Compared with wild-type, the OsEXPB7 down-regulation primary generation (T0) transgenic plants exhibited a shorter plant height and smaller size in grains. T1 transgenic seedlings showed retardant growth and drooping leaf phenotype. The growth defect could be partially compensated with gibberellin (GA) treatment. Under flooded conditions, the transgenic seedlings showed slower stem elongations than that of wild type plants. It suggested that OsEXPB7, probably regulated by GA, might play an role in influencing rice development through adjusting the extension of cell wall. Fig 9, Tab 3, Ref 30


Cosgrove DJ. Enzymes and other agents that enhance cell wall extensibility [J]. Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 391-417
荆赞革, 柳李旺, 龚义勤, 姜立娜, 孙新菊, 周艳孔. 萝卜扩展蛋白基因RsEXPB1克隆与表达特征分析[J]. 分子植物育种, 2009, 7 (4): 801-805 [Jing ZJ, Liu LW, Gong YQ, Jiang LN, Sun XJ, Zhou YK. Cloning and expression characterization of the expansin gene RsEXPB1 in radish [J]. Mol Plant Breeding, 2009, 7 (4): 801-805]
Chen F, Kent JB. Expression of an expansin is associated with endosperm weakening during tomato seed germination [J]. Plant Physiol, 2000, 124: 1265-1274
Chen F, Dahal P, Bradford KJ. Two tomato expansin genes show divergent expression and localization in embryos during seed development and germination [J]. Plant Physiol, 2001, 127: 928-936
Lee DK, Ahn JH, Song SK, Choi YD, Lee JS. Expression of an expansin gene is correlated with root elongation in soybean [J]. Plant Physiol, 2003, 131: 985-997
Cho HT, Cosgrove DJ. Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana [J]. Proc Natl Acad Sci USA, 2000, 97 (17): 9783-9788
Cosgrove DJ, Bedinger P, Durachko DM. GroupⅠallergens of grass pollen as cell wall-loosening agents [J]. Proc Natl Acad Sci USA, 1997, 94 (2): 6559-6564
Fleming AJ, McQueen-Mason S, Mandel T, Kuhlemeier C. Induction of leaf primordial by the cell wall protein expansin [J]. Science, 1997, 276 (5317): 1415-1418
Downes BP, Stenbaker CR, Crowell DN. Expression and processing of a hormonally regulated β-expansin from soybean [J]. Plant Physiol, 2001, 126: 244-252
Pezzotti M, Feron R, Marinai C. Pollination modulates expression of the PPAL gene, a pistil-specific β-expansin [J]. Plant Mol Biol, 2002, 49 (20): 187-197
Mbeguie AM, Gouble B, Gomez RM, Jean-Marc A, Guy A, Bernard FL. Two expansin cDNAs from Prunus armeniaca expressed during fruit ripening are differently regulated by ethylene [J]. Plant Physiol Biochem, 2002, 40: 445-452
Harrion EP, Mcqueen-Manning K. Expression of six expansin genes in relation to extension activity in developing strawberry fruit [J]. J Exp Bot, 2001, 52 (360): 1437-1446
Yoo SD, Gao ZF, Cantini C, Loescher WH, Nocker S. Fruit ripening in sour cherry: changes in expression of genes encoding expansins and other cell-wall-modifying enzymes [J]. J Am Soc Hortic Sci, 2003, 128: 16-22
Obenland DM, Crisosto CH, Rose JKC. Expansin protein levels decline with the development of mealiness in peaches [J]. Postharvest Biol Technol, 2003, 29: 1-18
Wu Y, Sharp RE, Druachko DM, Cosgrove DJ. Growth maintenance of the maize primary root at low water potentials involves increases in cell wall extensibility, expansins activity and wall susceptibility to expansins [J]. Plant Physiol, 1996, 111: 765-772
Ding XH, Cao YL, Huang LL, Zhao J, Xu CG, Li XH, Wang SP. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice [J]. Plant Cell, 2008, 20 (1): 228-240
Xu J, Tian J, Belanger FC, Huang B. Identification and characterization of an expansin gene AsEXP1 associated with heat tolerance in C3 Agrostis grass species [J]. J Exp Bot, 2007, 58 (13): 3789-3796
Li Y, Jones L, McQueen-Mason S. Expansins and cell growth [J]. Curr Opin Plant Biol, 2003, 6 (6): 603-610
金慧清, 陈英豪, 金勇丰. Expansin(细胞壁松弛蛋白)的发展[J]. 生命科学, 2006, 18 (2): 168-174 [Jin HQ, Chen YH, Jin YF. The development of expansin (cell wall expansion protein) [J]. Chin Bull Life Sci, 18 (2): 168-174]
Magneschi L, Kudahettige RL, Alpi A, Perata P. Expansin gene expression and anoxic coleoptile elongation in rice cultivars [J]. J Plant Physiol, 2009, 166: 1576-1580
Won SK, Choi SB, Kumari S, Cho M, Lee SH, Cho HT. Root hair-specific expansin β genes have been selected for graminaceae root hairs [J]. Mol Cells, 2010, 30: 369-376
Huang J, Takano T, Akita S. Expression of α-expansin genes in young seedlings of rice [J]. Planta, 2000, 211: 467-473
Lee Y, Kende H. Expression of α-expansin and expansin-like genes in deepwater rice [J]. Plant Physiol, 2002, 130: 1396-1405
Cho HT, Kende H. Expression of expansin genes is correlated with growth in deepwater rice [J]. Plant Cell, 1997, 9: 1661-1671
Choi D, Lee Y, Cho HT, Kende H. Regulation of expansin gene expression affects growth and development in transgenic rice plants [J]. Plant Cell, 2003, 15: 1386-1398
Lee Y, Choi D. Biochemical properties and localization of the β-expansin OsEXPB3 in rice (Oryza sativa L.) [J]. Mol Cells, 2005, 20 (1): 119-126
Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database [J]. Nucl Acids Res, 1999, 27 (1): 297-300
高永峰, 刘继恺, 范晶, 彭奕, 黄科, 张敦房, 刘永胜.水稻穗发芽调控基因OsVP1的RNA 干涉载体构建及遗传转化研究[J]. 中国水稻科学, 2010, 43 (7): 1321-1327 [Gao YF, Liu JK, Fan J, Peng Y, Huang K, Zhang DF, Liu YS. Construction and transformation of RNAi vector of OsVP1 for a regulatory gene of pre-harvest sprouting in Oryza sativa [J]. Sci Agric Sin, 2010, 43 (7): 1321-1327
Cho HT, Kende H. Expansins and intermodal growth of deepwater rice [J]. Plant Physiol, 1997, 113: 1145-1151
Lee Y, Kende H. Expression of beta-expansins is correlated with internodal elongation in deepwater rice [J]. Plant Physiol, 2001, 127: 645-654


Last Update: 2013-05-02