|Table of Contents|

Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2013 01
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Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology
LONG FeiJIN YanlingZHAO YunGAO XiaofengLI YuhaoCHEN QianZHAO Hai
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China )
(2University of Chinese Academy of Sciences, Beijing 100049, China)
(3Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China )
(4College of Life Sciences, Sichuan University, Chengdu 610064, China)
butanol raw Canna edulis Ker Clostridium acetobutylicum response surface methodology fermentation
TQ923 : S216

Raw Canna edulis Ker, one of the non-food crops, was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8012. Central Composite Design (CCD) was adopted to optimize experiment conditions including four crucial factors which were initial sugar concentration, inoculation volume, neutral red and ammonium acetate. According to the results of the model, the optimal conditions of butanol fermentation were that: initial sugar concentration 62.25 g/L, inoculation volume 10.81%, neutral red usage 0.81 g/L, ammonium acetate usage 0.574 g/L and final butanol yield of 12.83 g/L. In the verification experiment, the butanol yield of 12.73 g/L was obtained under the optimal conditions, which indicated that the model was reliable and effective.


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Last Update: 2013-02-26