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Structure Prediction and Function Analysis of Protein Phosphatase PPH1 from Arabidopsis thaliana(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2013 01
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Structure Prediction and Function Analysis of Protein Phosphatase PPH1 from Arabidopsis thaliana
HAN YongguangLUO YueWEI ZhengxiaoTANG QiujuDU Linfang
(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
PPH1 dephosphorylation PP2C core domain antibody

PPH1, a member of protein phosphatase 2C (PP2C) of Arabidopsis thaliana, is involved in the dephosphorylation of LHCII and the state transitions. The phosphorylation and dephosphorylation of LHCII appear to be specifically mediated by the kinase/phosphatase pair STN7 and PPH1. However, the catalytic mechanism and structure of PPH1 remain uncertain at present. In this research, the hydrophobic property, transmembrane domain, motif and secondary structure of PPH1 were discussed using a systematical bioinformatics method and a phylogenetic tree was constructed. Three dimensional structures of core domains of PPH1 were predicted by using homologous modeling method. On the basis of the above analysis, one anti-peptide antibody was designed. Western blot analysis with thylakoid membranes of A. thaliana showed the polyclonal antibodies against the peptides were able to act with the parent protein. Therefore, structure prediction of the PPH1 provided the foundation for further studying the functions and designing mutant of PPH.


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Last Update: 2013-02-26