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Glycerol Synthesis Attenuation of Candida glycerinogenes in Fed-batch Fermentation(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 05
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Glycerol Synthesis Attenuation of Candida glycerinogenes in Fed-batch Fermentation
DING Xiaoyun ZHUGE Bin FANG Huiying ZONG Hong LIU Xiaoxiao WANG Yuqian ZHUGE Jian
(1Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China)
(2State Key Laboratory of Food Science and Technology of China, Jiangnan University, Wuxi 214122, Jiangsu, China)
(3Research Center of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China)
Candida glycerinogenes fed-batch fermentation glycerol synthesis attenuation metabolic flux key enzyme
TQ920.1 : TQ923

The previous resulted showed that in fed-batch fermentation of Candida glycerinogenes, the glycerol concentration decreased by 13%, the conversion ratio of glycerol decreased by 34%, the glycerol synthesis rate decreased by 67% and the glucose consumption rate decreased by 44%. In order to reveal the mechanism of glycerol synthesis attenuation, the glycerol synthesis, glucose consumption, intracellular metabolic flux distribution and the activity of key enzymes in metabolism were investigated. It was found that the metabolic flux distribution changed compared with batch fermentation. The metabolic flux decreased by 48% and 33% respectively in HMP and glycerol synthesis pathway while the metabolic flux increased in EMP, which led to the increase of by-products. The activity of ctGPD and G6PDH decreased, while the activity of PYK increased. ctGPD, a key enzyme for glycerol formation, directly affected the glycerol formation. G6PDH influenced the carbon flux to HMP, which not only played an important role in cell growth, but also provided reducing power for glycerol formation. PYK influenced the metabolic flux distribution between EMP and glycerol synthesis pathway. Consequently, the decrease in the activity of G6PDH and carbon flux to glycerol synthesis pathway, and inefficient reducing power for glycerol formation showed synergy effects on the glycerol synthesis attenuation in fed-batch fermentation. The results should provide the primary data-materials for the regulation of glycerol synthesis in C. glycerinogenes and strain improvement. Fig 4, Tab 2, Ref 22


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Last Update: 2012-10-26